Close Relationship between G8-Serotype Bovine and Human Rotaviruses Isolated in Nigeria

Abstract

A bovine rotavirus, NGRBg8, isolated from the feces of a calf with diarrhea in Nigeria was characterized by reverse transcription-PCR, nucleotide sequence analysis, and Northern blot hybridization. The nucleotide sequence of the VP7 gene of the strain was most closely related to that of a Nigerian human G8-serotype strain, HMG035 (99.9%). The NSP1 gene of strain NGRBg8 is highly related (99.4%) to that of a Thai G8 bovine strain, A5-10. Northern blot hybridization revealed a high overall genomic relatedness of bovine strain NGRBg8 with human strain HMG035; all 11 RNA segments hybridized to each other. Thus, the results show the close relationship between G8 bovine and human rotaviruses in Nigeria.

G8Rotavirus Strains Isolated in the Democratic Republic of Congo Belong to the DS-1-Like Genogroup

“…1A and B). A very high degree of P-type diversity (P [1], P [4], P [6], and P [8]) was noticed for these eight G8 RV strains (Fig. 1B).…”

“…The exact number of nucleotides (amino acids) were as follows: 3,302 (1,088) for VP1, 2,687 (880) G-typing analysis of the VP7 gene. The VP7 genes of RV strains DRC88 and DRC86 showed a 96.2 to 97.7% nucleotide sequence identity and a 96.8 to 98.1% amino acid sequence identity with the following G8 RV strains: MW333 and MW23 isolated in Malawi, R291 isolated in Brazil, and human and bovine strains HMG035, HMG89, and NGRBg8 isolated in Nigeria (1)(2)(3)12). Phylogenetic analysis of VP7 clustered DRC88 and DRC86 with these five African strains and one Brazilian RV strain (Fig.…”

“…By far, the most G8 HRVs were found all over the African continent (Malawi, Nigeria, Egypt, Kenya, South Africa, Guinea-Bissau, and Ghana), and in a few epidemiological surveys, G8 was the predominant G genotype (3,7,12,13,18,19,26,44,63). G8 HRVs have been detected in combination with a large number of VP4 specificities: P [1], P [2], P [4], P [6], P [8], P [10], and P [14] (13,19,22,31,33,53,61). In addition to this list, P [5] and P [11] (typical bovine P genotypes) have been found in combination with G8 in cattle (17,20,49).…”

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

“…Since their discovery, serotype G8 (in association with serotypes/genotypes P1A [8], P1B [4], P2A [6], P4 [10], P6 [1], or P11 [14]) rotavirus strains have been isolated sporadically from children in several countries and have been established, after serotypes G6 and G10, as the third most common G type (in association with serotypes/genotypes P6 [1], P7 [5], P8 [11], or P11 [14]) found in cattle [24,[34][35][36][37][38][39][40][41][42][43][51][52][53]. In other animal species, a single equine rotavirus strain, 26/94, possessing P6 [1]G8 specificities has been identified [49], as well as a single simian rotavirus strain, YK-1, with the same specificities [54].…”

“…Two potential N-linked glycosylation sites located at aa 69 and 238 were found, as in all other serotype G8 HRV and bovine rotavirus (BRV) strains, with the exception of strains that lack the glycosylation site at position 69 (BRV 678) and at position 238 (guanaco rotavirus [GRV] strain Río Negro) [24,[34][35][36][37][38][39][40][41][42][43] (Fig. 2).…”