G8Rotavirus Strains Isolated in the Democratic Republic of Congo Belong to the DS-1-Like Genogroup

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

“…OVR strains have been isolated in the United Kingdom, Japan, and China, but only those isolated from the United Kingdom and China have been characterized [3,13,[16][17][18][19][20]. Indeed, the most well- [25] P [8] P [4] P [19] P [6] P [27] P [5] P [12] P [10] P [21] P[15] P [24] P [7] P [13] P [20] P [16] P [3] P [2] P [18] P [1] P [17] P [11] P [26] P [14] 0.05 nucleotide substitutions per bp A Fig. 4 The present study constitutes the first report of an OVR strain, 762, with P [14] and G8 specificities.…”

“…Two potential N-linked glycosylation sites located at aa 69 and 238 were found, as in all other serotype G8 HRV and bovine rotavirus (BRV) strains, with the exception of strains that lack the glycosylation site at position 69 (BRV 678) and at position 238 (guanaco rotavirus [GRV] strain Río Negro) [24,[34][35][36][37][38][39][40][41][42][43] (Fig. 2).…”

“…Reverse transcription of dsRNA was carried out using MuLV-reverse transcriptase (Perkin-Elmer Europe B.V. Monza) while PCR amplification was carried out with AmpliTaq Gold Ò DNA polymerase (Perkin-Elmer Europe B.V. Monza). The VP8* subunit of the VP4, the connecting peptide, and the N terminus of the VP5* subunit of VP4 (876 base pairs [bp]), predictive of the P genotype, were reverse transcribed and amplified as described [24,25]. The fulllength sequence of VP4 (2,361 bp) was determined by using selected consensus 3 0 end and internal primers as described elsewhere [24].…”

“…The VP8* subunit of the VP4, the connecting peptide, and the N terminus of the VP5* subunit of VP4 (876 base pairs [bp]), predictive of the P genotype, were reverse transcribed and amplified as described [24,25]. The fulllength sequence of VP4 (2,361 bp) was determined by using selected consensus 3 0 end and internal primers as described elsewhere [24]. The full-length VP7 gene (1,062 bp) was reverse transcribed and amplified using primer Beg9 [26] and the 3 0 degenerated primer End9deg (5 0 -GGTCACATCDWMCARYTCTAAYYHM-3 0 ).…”

“…The VP6 genogroup was determined by amplification of a 379-bp fragment, spanning from amino acid (aa) 241-367 of the VP6, as described [4]. In addition, the complete VP6 gene segment (1,355 bp) was amplified as described elsewhere [24]. The full-length gene (genome segment 10, 751 bp) encoding for the NSP4 protein and the NSP5/NSP6 fulllength gene (genome segment 11, 667 bp) were amplified as described [19,27].…”