Cloprostenol and pregnant mare serum gonadotropin promote estrus synchronization, uterine development, and follicle-stimulating hormone receptor expression in mice

Wei, Shaozhong; Gong, Zhuandı; An, Lifeng; Zhang, T; Dai, Huaping; Chen, S

doi:10.4238/2015.june.29.12

Cited by 10 publications

(4 citation statements)

References 19 publications

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“…In addition, the expression of GHR was not correlated with the change of insulin-like growth factor (IGF-I) concentration postpartum in the liver [20]. FSHR has significance regarding the modulation of animal reproductive functions [10,23]. Moreover, previous studies found that the FSH could accelerate the maturation rate of sheep oocytes and reduce their apoptosis rate, increase the expression levels of FSHR, LHR and gonadotropin releasing hormone receptor (GnRHR) mRNAs, and strengthen expressions of FSHR and GnRHR proteins [21].…”

Section: Introductionmentioning

confidence: 99%

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Chen¹,

Cui²,

Yu³

2018

Folia Morphol

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Section: Introductionmentioning

confidence: 99%

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Chen¹,

Cui²,

Yu³

2018

Folia Morphol

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“…Oestrogen deficiency has been suggested to be the predominant cause of post-menopausal osteoporosis ( 1 ). Accompanied by the withdrawal of sex steroids in mammals, serum levels of the pituitary hormone follicle-stimulating hormone (FSH) are markedly increased ( 2 , 3 ). FSH exhibits osteoclastogenic and proresorptive actions in a Gi2α-coupled FSH receptor (FSHR) identified in human and mouse osteoclasts and their oestrogen-independent precursors ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

FSH aggravates bone loss in ovariectomised rats with experimental periapical periodontitis

Qian

Guan

Bian

2016

Molecular Medicine Reports

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Periapical bone loss is one of the prominent pathological and clinical features of periapical periodontitis. Previous studies have demonstrated that follicle-stimulating hormone (FSH) could directly affect skeletal remodelling by stimulating the formation and the function of osteoclasts in vitro and in vivo. However, the effect of FSH on periapical bone loss remained to be fully elucidated. In the current study, a rat model was established in order to verify the effect of FSH in experimental periapical lesions. It was identified that FSH aggravated the bone loss of periapical lesions. In addition, RANKL-, TRAP-, TNF-α- and IL-1β-positive cells were increased significantly in FSH-treated groups, which indicated that the function of FSH in bone loss may be mediated through the increasing activity of osteoclasts and the increased secretion of inflammatory cytokines. The results of the current study suggested that FSH, independent of oestrogen, may aggravate periapical bone loss by FSH receptors, which may serve an important role in the immune and inflammatory response of the host to root canal and periradicular infection during menopause.

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“…Our previous investigation demonstrated that synthesis and secretion of LH and FSH are increased in mice following intramuscular injection of 10, 20, or 40 IU eCG (previously known as PMSG) (Wei et al, 2015). In the current study, we showed that expression of FSHR, LHR, and GnRHR mRNA and protein increased as the concentration of eCG in IVM medium was raised.…”

Section: Discussionmentioning

confidence: 99%

Equine chorionic gonadotropin influence on sheep oocyte in vitro maturation, apoptosis, and follicle-stimulating hormone receptor and luteinizing hormone receptor expression

et al. 2016

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We assessed the effects of equine chorionic gonadotropin (eCG) on oocyte in vitro maturation (IVM), apoptosis, and follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and gonadotropin-releasing hormone receptor (GnRHR) expression and mRNA levels. Cumulus-oocyte complexes (COCs) were recovered from sheep ovaries and pooled in groups, before being cultured in IVM media containing varying eCG concentrations. Maturation and apoptosis rates were then calculated. Expression of FSHR, LHR, and GnRHR mRNA in oocytes was measured using quantitative reverse transcription polymerase chain reaction. Protein levels were ascertained by western blotting. Matured oocytes displayed and released an intact first polar body. Sheep oocyte maturation rates gradually increased as eCG concentration was raised from 0 to 20 µg/mL. Apoptosis rates of eCG-treated oocytes were lower than those of the control group, and were lowest using 20 µg/mL eCG. FSHR, LHR, and GnRHR mRNA expression increased (P < 0.01, P < 0.05, and P < 0.05, respectively, compared to 0 µg/mL eCG) with eCG concentration, being highest following exposure to 20 µg/mL. FSHR and GnRHR protein levels were significantly higher in oocytes administered 20 µg/mL eCG compared with those matured in the absence of eCG. eCG dose positively correlated with FSHR, LHR, and GnRHR mRNA and protein expression. In conclusion, eCG enhances maturation and decreases apoptosis of oocytes undergoing IVM, and heightens FSHR, LHR, and GnRHR expression. Such increased expression may facilitate oocyte IVM. These findings contribute to our understanding of the mechanisms of underlying hormonal control of sheep oocyte IVM, advancing ovine reproductive methods.

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Cloprostenol and pregnant mare serum gonadotropin promote estrus synchronization, uterine development, and follicle-stimulating hormone receptor expression in mice

Cited by 10 publications

References 19 publications

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

Expression and localisation of FSHR, GHR and LHR in different tissues and reproductive organs of female yaks

FSH aggravates bone loss in ovariectomised rats with experimental periapical periodontitis

Equine chorionic gonadotropin influence on sheep oocyte in vitro maturation, apoptosis, and follicle-stimulating hormone receptor and luteinizing hormone receptor expression

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