Cloprostenol and eCG influence oestrus synchronisation and uterine development in mice

Abstract

ABSTRACT:The aim of this study was to investigate the efficacy of equine chorionic gonadotropin (eCG) and cloprostenol (CLO) administration on oestrus synchronisation, uterine development as well as serum LH and FSH concentrations in mice. One hundred and five KM mice were assigned into eCG-1, eCG-2, eCG-3, CLO-1, CLO-2, CLO-3 and control groups (CG, n = 15). The eCG-1, eCG-2 and eCG-3 groups were intramuscularly injected with 10, 20 and 40 IU eCG. CLO-1, CLO-2 and CLO-3 were intramuscularly injected with 10, 15 and 20 μg cloprostenol acetate. The results showed that 93.33% and 66.67% of synchronised mice displayed oestrus within 18.68-37.59 h. Oestrus numbers, oestrus onset time (EOT) and oestrus rate in CLO and eCG groups were greater than in CG (P < 0.05). EOT in CLO and eCG groups were 19.88 ± 2.91 h and 34.84 ± 5.05 h. Uterine weights of treatment groups were larger than CG. Uterine weights of the eCG group were higher than those in the CLO group. Uterine horn longitudial diameters (ULD) in treatment groups were larger than CG during the experiment. ULD in eCG-2 and eCG-3 were significantly greater when compared to CG (P < 0.05 or P < 0.01). On days 14 and 21, uterine horn transverse diameters (UTD) in CLO-1, eCG-1, eCG-2 and eCG-3 subgroups were significantly larger than that of CG (P < 0.05 or P < 0.01). Serum LH concentrations in eCG and CLO increased. Increments in eCG and CLO groups were greater than that of CG. FSH concentrations in eCG mice were higher than those in CLO and CG mice (P < 0.05) on day 21. Thus, eCG and cloprostenol treatments in mice can improve uterine development and promote the secretion of LH and FSH.

Ovum pick‐up and in vitro maturation in spotted paca (Cuniculus paca—Linnaeus, 1766)

“…However, these results were not achieved in guinea pig (Grégoire et al, ) or agouti, showing limitations in oestrous induction (Peixoto et al, ). In addition, research has shown that the administered dose of this hormone may affect oestrous synchronization efficiency (Wei et al, ).…”

“…Gonadotropins such as FSH and eCG have been used for ovarian stimulation protocols in several species, but have never been tested in large rodents. Comparing those hormones in superovulation protocols, it was observed that mice responded better to eCG than to FSH (Corbin & McCabe, ) which showed to be very effective for both oestrous induction and ovarian hyperstimulation (Agca, Yakan, & Agca, ) especially if combined with cloprostenol (Wei et al, ). However, FSH and eCG showed similar efficiency in some rat strains (Popova, Bader, & Krivokharchenko, ).…”

Morphometric and histopathologic evaluation of the effects of cloprostenol and equine chorionic gonadotropin administration on the reproductive organs of female rats

“…It is recommended that prostaglandin F 2 -alpha (PGF 2 α), alone or in combination with a hormone that stimulates follicle development, be used as a luteolytic agent to increase reproductive efficiency and the pregnancy rate. However, this is generally costly and may not produce the expected effect in some cases (11,27).…”

“…Cloprostenol is a potent PGF 2 α analogue. Therefore, reproduction may be controlled by the lysis of the corpus luteum and by inducing a subsequent follicular phase with ovulation (27). Equine chorionic gonadotropin, which is a gonadotropic hormone produced in the chorion of pregnant mares, is widely used to obtain a large number of oocytes by the induction of superovulation in many animals (13,(18)(19)(20)(21)25).…”

“…Equine chorionic gonadotropin, which is a gonadotropic hormone produced in the chorion of pregnant mares, is widely used to obtain a large number of oocytes by the induction of superovulation in many animals (13,(18)(19)(20)(21)25). In mice, eCG and cloprostenol can promote the secretion of FSH and LH (27). However, there is a large variation in response to hormone stimulation even within species (6).…”