Ovum pick‐up and in vitro maturation in spotted paca (Cuniculus paca—Linnaeus, 1766)

Abstract

We tested FSHp, eCG and FSHp + eCG to establish ovum pick‐up (OPU) and in vitro maturation method in spotted paca. Eight healthy adult females were subjected to each of four treatments to stimulate ovarian follicular growth. All females were subjected to a hormonal protocol using a single dose of 45 mg of injectable progesterone and single intramuscular injection of 0.075 mg d‐cloprostenol on day 6. Ovarian stimulation was carried out as follows: in Group TFE (FSHp and eCG), animals were treated with a single dose of 80 mg of FSHp and 200 IU of eCG intramuscularly on day 6 after the application of progesterone; in Group TF (FSHp), they were treated with a single dose of 80 mg of FSHp intramuscularly on day 6 after application of progesterone; in Group treatment eCG, they were treated with 200 IU of eCG intramuscularly on day 6 after application of progesterone; and in Group TC (saline solution), 1 ml of saline solution was administered to control does. The OPU was performed between 22 and 26 hr after gonadotropin treatments. All recovered oocytes were placed into maturation media and incubated for 24 hr. There were no differences among the mean number of observed follicles, aspirated follicles and oocytes recovered per treatment. Oocyte maturation rates did not differ among groups, except, TF and treatment eCG oocytes had greater maturation rates than TC oocytes. In this study, gonadotropin administration failed to superovulate treated does and increase oocyte retrieval efficiency. Despite the feasibility of the procedure, further studies are needed to develop and refine hormonal protocols for oocyte recovery and in vitro maturation in this species.

Synergistic effects of follicle‐stimulating hormone and epidermal growth factor on in vitro maturation and parthenogenetic development of red‐rumped agouti oocytes

“…FSH has been shown to support nuclear and cytoplasmic maturation, consequently increasing IVM rates (Roberts et al, 2005), and this effect occurs dose‐dependently (Lu et al, 2000). In rodents, the most commonly used FSH concentration is 75 mIU/mL, as observed in mice (Lin et al, 2011) and pacas ( Cuniculus paca , Barros et al, 2020). However, this dosage is considered above the physiological limit (Lin et al, 2011), which can lead to chromosomal abnormalities (Roberts et al, 2005).…”