Cloning the PvP5CS gene from common bean (Phaseolus vulgaris) and its expression patterns under abiotic stresses

Chen, Jibao; Wang, Shumin; Jing, Ruilian; Mao, Xinguo

doi:10.1016/j.jplph.2008.02.010

Cited by 87 publications

(45 citation statements)

References 27 publications

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“…P5CS genes have since been cloned from A. thaliana [43], Medicago sativa [44], Lycopersicon esculentum [45], Medicago truncatula [46] and other species [47][48][49][50]. P5CS genes have been extensively studied in glycophytes that apparently lack the Pro tolerance mechanism; however, only a small number of studies have been done in halophytes [51].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Zheng

Dang

et al. 2013

Mol Biol Rep

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Several functional and regulatory proteins play important roles in controlling plant stress tolerance. Proline (Pro) is one of the most accumulated osmolytes correlated with tolerance to stresses. Δ(1)-Pyrroline-5-carboxylate synthetase (P5CS) is a rate-limiting enzyme in Pro biosynthesis. In the present study, we isolated the cDNA for a P5CS gene (NtP5CS) from the halophyte Nitraria tangutorum. Phylogenetic analysis and subcellular localization analysis of NtP5CS-GFP protein in onion cells showed that NtP5CS was a new P5CS gene and was involved in Pro synthesis in N. tangutorum. Expression of the NtP5CS gene was induced by salt stress, dehydration, and high and low temperatures. Escherichia coli overexpressing AtP5CS or NtP5CS exhibited better growth in all treatments, including high salinity, high alkalinity, dehydration, osmotic, heat and cold stresses. Additionally, NtP5CS recombinant E. coli cells grew better than did AtP5CS recombinant cells in response to abiotic stresses. Our data demonstrate that the P5CS from a halophytic species functions more efficiently than its homologue from a glycophytic species in improving the stress tolerance of E. coli.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Zheng

Dang

et al. 2013

Mol Biol Rep

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“…Δ 1 -pyrroline-5-carboxylase synthase (P5CS) is a key regulatory enzyme involved in the biosynthesis of proline. P5CS transcript levels are increased by overexpression of AtCBF3/DREB1A in Arabidopsis (Gilmour et al 2000) and by cold treatment in rice (Igarashi et al 1997) and common beans (Chen et al 2009). SlICE1 also enhanced P5CS expression in tomato red fruits ( Figure 4B), leading to the accumulation of proline.…”

Section: Increase In the Accumulation Of Sugars And Amino Acids In Slmentioning

confidence: 99%

Accumulation of antioxidants and antioxidant activity in tomato, Solanum lycopersicum, are enhanced by the transcription factor SlICE1

Miura

Sato

Shiba

et al. 2012

Plant Biotechnology

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Antioxidants and antioxidant activity confer important protective e ects in plants against the e ects of free radicals, which are generated by biotic and abiotic stresses, such as cold. In another study, we identi ed SlICE1 as a basic helix-loop-helix transcription factor to improve cold tolerance. Here, we demonstrate that SlICE1 plays an important role in the accumulation of antioxidants and in the regulation of antioxidant activity in tomato Solanum lycopersicum. Overexpression of SlICE1 in tomatoes enhanced the accumulation of antioxidants, such as β-carotene, lycopene, and ascorbic acid, as well as antioxidant activity, measured as the scavenging of 2,2-diphenyl-1-picrylhydazyl (DPPH) free radicals and O 2 − radicals. Furthermore, the sugar content in SlICE1-overexpressing tomatoes red fruits was higher than that in wild-type red fruits. Metabolite pro ling analysis performed by capillary electrophoresis time-of-ight mass spectrometry (CE-TOFMS) revealed that several amino acids and amines were more highly accumulated in SlICE1-overexpressing tomato red fruits compared to those in wild-type tomatoes. ese results suggest that SlICE1 plays a role in the regulation of antioxidant activity through the accumulation of several antioxidants.

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“…Our results observed that only 24 out of 100 (24%) of putatively transformed grape plants with pBI121 contained the P5CS gene and were confirmed to be transgenic by PCR. In previous studies, researchers have attempted to focus on P5CS expression patterns in higher plants and they found increasing levels of constitutive transcript abundance compared with the genes VaP5CS (de Carvalho et al, 2013), OsP5CS (Igarashi et al, 1997), PvP5CS (Chen et al, 2009), JcP5CS (Wang et al, 2011), AtP5CS1 (Zhuang et al, 2011) and MsP5CS1 (Fichman et al, 2014). Induction of transcript expression of P5CS gene has also been reported in response to abiotic stresses (Neffar et al, 2011) and it was found to encourage proline synthesis and improved stress tolerance (Vendruscolo et al, 2007;Patade et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The glutamate pathway is considered to be the major path for proline synthesis under harsh conditions. In several plant species, Phaseolus vulgaris (Chen et al, 2009), Medicago truncatula (Armengaud et al, 2004), Glycine max (Mau et al, 2010), reed canary grass (Cong et al, 2014) and sugar cane (Iskandar et al, 2014), cloning the P5CS gene and its over-expression results in increased abiotic stress tolerance but there are no reports in the literature for grapes.…”

Section: Introductionmentioning

confidence: 99%

Producing Transgenic Thompson Seedless Grape (Vitis vinifera) Plants using Agrobacterium tumefaciens

Metwali¹,

Soliman²,

Almaghrabi³

et al. 2016

IJAB

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Under osmotic stress plant avoid it by different modification in their metabolisms such as increasing the synthesis of mRNA of delta 1-pyrroline-5-carboxylate synthase (P5CS), where P5CS is a target gene for increasing proline production and expect to improve the resistance to abiotic stress. To test this hypothesis, grape (Vitis vinifera L.) cv. Thompson Seedless was conducted to develop a protocol for high frequency regeneration and Agrobacterium mediated P5CS gene transfer. Callus induction was achieved by culture leaf explants on Nitsch and Nitsch (NN) basal medium including 2.0 mg L -1 2,4-di-chloro-phenoxy-aceticacid + 0.5 mg L -1 Thiadiazuron solidified with 2.5 g L -1 phytagel. While for in vitro proliferation of plant, the calli were cultured on NN medium supplemented with 3.0 mg L -1 Zeatin riboside + 0.5 mg L -1 Thiadiazuron. Agrobacterium-mediated transformation using the strain LB4404 harbouring the binary vector pBI121 with the P5CS gene under CaMV 35S promoter and the bar gene as a plant selectable marker was used for transforming grape explants. A P5CS specific band (2100 bp) was amplified from DNA extracted only from the transgenic grape plants and 24% for P5CS gene was positive for the bar gene. Over expression of the abiotic stress P5CS gene was enhanced synthesis of proline to over 6 times higher in transgenic plants compared to controls. The successful transformation of genetically diverse grape cv. Thompson Seedless indicated that the transformation system may have general application to an even wider range of grapes cultivars.

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Cloning the PvP5CS gene from common bean (Phaseolus vulgaris) and its expression patterns under abiotic stresses

Cited by 87 publications

References 27 publications

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Accumulation of antioxidants and antioxidant activity in tomato, Solanum lycopersicum, are enhanced by the transcription factor SlICE1

Producing Transgenic Thompson Seedless Grape (Vitis vinifera) Plants using Agrobacterium tumefaciens

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