Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Zheng, Linlin; Dang, Zhenhua; Li, Haoyu; Zhang, Huirong; Wu, Shu‐Biao; Wang, Yingchun

doi:10.1007/s11033-013-2893-8

Cited by 29 publications

(17 citation statements)

References 56 publications

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“…Previous studies reported that during salinity stress, synthesis of osmo-regulators was the highest in tolerant genotypes to maintain favorable plant water status inside cells (Karthikeyan et al, 2011;Li et al, 2011;Zang et al, 2011). Interestingly, when we checked the contents of most common organic osmolytes, significantly higher accumulation of trehalose, proline, and GB was found in FL478, but in Kamini, it was at par with susceptible genotypes.…”

Section: Salt-tolerant Genotype Kamini Balances Both Ion-exclusion Anmentioning

confidence: 70%

Tissue Tolerance Coupled With Ionic Discrimination Can Potentially Minimize the Energy Cost of Salinity Tolerance in Rice

et al. 2020

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Salinity is one of the major constraints in rice production. To date, development of salt-tolerant rice cultivar is primarily focused on salt-exclusion strategies, which incur greater energy cost. The present study aimed to evaluate a balancing strategy of ionic discrimination vis-à-vis tissue tolerance, which could potentially minimize the energy cost of salt tolerance in rice. Four rice genotypes, viz., FL478, IR29, Kamini, and AC847, were grown hydroponically and subjected to salt stress equivalent to 12 dS m −1 at early vegetative stage. Different physiological observations (leaf chlorophyll content, chlorophyll fluorescence traits, and tissue Na + and K + content) and visual scoring suggested a superior Na +-partitioning strategy operating in FL478. A very low tissue Na + /K + ratio in the leaves of FL478 after 7 days of stress hinted the existence of selective ion transport mechanism in this genotype. On the contrary, Kamini, an equally salt-tolerant genotype, was found to possess a higher leaf Na + /K + ratio than does FL478 under similar stress condition. Salt-induced expression of different Na + and K + transporters indicated significant upregulation of SOS, HKT, NHX, and HAK groups of transporters in both leaves and roots of FL478, followed by Kamini. The expression of plasma membrane and vacuolar H + pumps (OsAHA1, OsAHA7, and OsV-ATPase) were also upregulated in these two genotypes. On the other hand, IR29 and AC847 showed greater salt susceptibility owing to excess upward transport of Na + and eventually died within a few days of stress imposition. But in the "leaf clip" assay, it was found that both IR29 and Kamini had high tissue-tolerance and chlorophyll-retention abilities. On the contrary, FL478, although having higher ionic-discrimination ability, showed the least degree of tissue tolerance as evident from the LC 50 score (amount of Na + required to reduce the initial chlorophyll content to half) of 336 mmol g −1 as against 459 and 424 mmol g −1 for IR29 and Kamini, respectively. Overall, the present study indicated that two components (ionic selectivity and tissue tolerance) of salt tolerance mechanism are distinct in rice. Unique genotypes like Kamini could effectively balance both of these strategies to achieve considerable salt tolerance, perhaps with lesser energy cost.

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Section: Salt-tolerant Genotype Kamini Balances Both Ion-exclusion Anmentioning

confidence: 70%

Tissue Tolerance Coupled With Ionic Discrimination Can Potentially Minimize the Energy Cost of Salinity Tolerance in Rice

et al. 2020

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“…P5CS enzyme activity in sorghum ( Sorghum bicolor ) seedlings was found to be induced upon short-term heat stress (6 h at 40 °C) 52 , but inhibited under long-term heat stress, such as in wheat (5 d at 33 °C) 53 , and tomato ( Solanum lycopersicum ) (48 h at 35 °C) 54 . Similarly, transcript levels of P5CS were up-regulated by short-term heat stress in Nitraria tangutorum (6 h at 50 °C) 55 , but down-regulated by long- term heat stress in peach ( Prunus persica ) (5 d at 36.7 °C) 56 . The common up-regulation of proline synthesis by both long-term (21 d) heat and drought stress in this study suggested the importance of P5CS regulation of proline synthesis in A. stolonifera adaptation to prolonged heat and drought stress.…”

Section: Discussionmentioning

confidence: 96%

Comparative transcriptomic analysis reveals common molecular factors responsive to heat and drought stress in Agrostis stolonifera

Huang

2018

Sci Rep

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Heat and drought stress are primary abiotic stresses confining growth of cool-season grass species during summer. The objective of this study was to identify common molecular factors and metabolic pathways associated with heat and drought responses in creeping bentgrass (Agrostis stolonifera) by comparative analysis of transcriptomic profiles between plants exposed to heat and drought stress. Plants were exposed to heat stress (35/30 °C day/night temperature) or drought stress by withholding irrigation for 21 d in growth chambers. Transcriptomic profiling by RNA-seq in A. stolonifera (cv. ‘Penncross’) found 670 commonly up-regulated and 812 commonly down-regulated genes by heat and drought stress. Transcriptional up-regulations of differentially expressed genes (DEGs) due to heat and drought stress include genes that were highly enriched in oxylipin biosynthetic process and proline biosynthetic process. Transcriptional down-regulations of genes under heat and drought stress were highly enriched and involved in thiamine metabolic process and calcium sensing receptor. These commonly-regulated genes by heat and drought stress identified in A. stolonifera suggested that drought and heat responses shared such common molecular factors and pathways, which could be potential candidate genes for genetic modification of improving plant tolerance to the combined heat and drought stress.

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“…In the current study, the abundance in Δ 1 -pyyroline-5-carboxylate synthase (Bra005012) was decreased in resistant samples. This enzyme catalyzes the rate-limiting step of proline biosynthesis responding to environmental stresses (Zheng et al, 2014 ). Even two of the enzymes involved in arginine biosynthesis were up-regulated, the down-regulation of this key enzyme in proline biosynthesis may indicate that the P. brassicae -induced arginine catabolism and proline biosynthesis are both disrupted in resistant samples carrying Rcr1 .…”

Section: Discussionmentioning

confidence: 99%

Shotgun Label-free Proteomic Analysis of Clubroot (Plasmodiophora brassicae) Resistance Conferred by the Gene Rcr1 in Brassica rapa

et al. 2016

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Clubroot, caused by the plasmodiophorid pathogen Plasmodiophora brassicae, is one of the most serious diseases on Brassica crops worldwide and a major threat to canola production in western Canada. Host resistance is the key strategy for clubroot management on canola. Several clubroot resistance (CR) genes have been identified, but the mechanisms associated with these CR genes are poorly understood. In the current study, a label-free shotgun proteomic approach was used to profile and compare the proteomes of Brassica rapa carrying and not carrying the CR gene Rcr1 in response to P. brassicae infection. A total of 527 differentially accumulated proteins (DAPs) were identified between the resistant (with Rcr1) and susceptible (without Rcr1) samples, and functional annotation of these DAPs indicates that the perception of P. brassicae and activation of defense responses are triggered via an unique signaling pathway distinct from common modes of recognition receptors reported with many other plant–pathogen interactions; this pathway appears to act in a calcium-independent manner through a not-well-defined cascade of mitogen-activated protein kinases and may require the ubiquitin-26S proteasome found to be related to abiotic stresses, especially the cold-stress tolerance in other studies. Both up-regulation of defense-related and down-regulation of pathogenicity-related metabolism was observed in plants carrying Rcr1, and these functions may all contribute to the CR mediated by Rcr1. These results, combined with those of transcriptomic analysis reported earlier, improved our understanding of molecular mechanisms associated with Rcr1 and CR at large, and identified candidate metabolites or pathways related to specific resistance mechanisms. Deploying CR genes with different modes of action may help improve the durability of CR.

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Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue

Cited by 29 publications

References 56 publications

Tissue Tolerance Coupled With Ionic Discrimination Can Potentially Minimize the Energy Cost of Salinity Tolerance in Rice

Tissue Tolerance Coupled With Ionic Discrimination Can Potentially Minimize the Energy Cost of Salinity Tolerance in Rice

Comparative transcriptomic analysis reveals common molecular factors responsive to heat and drought stress in Agrostis stolonifera

Shotgun Label-free Proteomic Analysis of Clubroot (Plasmodiophora brassicae) Resistance Conferred by the Gene Rcr1 in Brassica rapa

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