Cloning of zebrafish activin type IIB receptor (ActRIIB) cDNA and mRNA expression of ActRIIB in embryos and adult tissues

Garg, Rami R.; Bally‐Cuif, Laure; Lee, S.E; Gong, Zhiyuan; Ni, Xueying; Hew, CL; Peng, Chun

doi:10.1016/s0303-7207(99)00044-1

Cited by 43 publications

(22 citation statements)

References 59 publications

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“…(Fig.1), and all 10 cysteine residues conserved in the extracellular domain of other Acvr2b proteins (Garg et al, 1999) were present.…”

Section: Rainbow Trout Acvr2b-2a Cdsmentioning

confidence: 95%

“…The zebrafish acvr2b-2a sequence was used for production of transgenic RBT because of its detailed molecular characterization (Garg et al, 1999) and conservation to RBT acvr2b-2a (89% amino acid conservation of the extracellular domain). Using the zebrafish acvr2b-2a sequence also enabled differentiation from the native RBT receptor transcript for precise quantification of transgene expression levels.…”

Section: Dna Constructmentioning

confidence: 99%

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Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor

Phelps

Jaffe

Bradley

2013

Journal of Experimental Biology

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SUMMARYThe activin type IIB receptor (Acvr2b) is the cell surface receptor for multiple transforming growth factor β (TGF-β) superfamily ligands, several of which regulate muscle growth in mammals. To investigate the role of the Acvr2b signaling pathway in the growth and development of skeletal muscle in teleost fish, transgenic rainbow trout (RBT; Oncorhynchus mykiss) expressing a truncated form of the acvr2b-2a (acvr2b ∆ ) in muscle tissue were produced. High levels of acvr2b ∆ expression were detected in the majority of P1 transgenic fish. Transgenic P1 trout developed enhanced, localized musculature in both the epaxial and hypaxial regions (dubbed 'six pack'). The F1 transgenic offspring did not exhibit localized muscle growth, but rather developed a uniform body morphology with greater girth, condition factor and increased muscle fiber hypertrophy. There was a high degree of variation in the mass of both P1 and F1 transgenic fish, with several fish of each generation exhibiting enhanced growth compared with other transgenic and control siblings. The 'six pack' phenotype observed in P1 transgenic RBT overexpressing acvr2b ∆ and the presence of F1 individuals with altered muscle morphology provides compelling evidence for the importance of TGF-β signaling molecules in regulating muscle growth in teleost fish.

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“…(Fig.1), and all 10 cysteine residues conserved in the extracellular domain of other Acvr2b proteins (Garg et al, 1999) were present.…”

Section: Rainbow Trout Acvr2b-2a Cdsmentioning

confidence: 95%

Section: Dna Constructmentioning

confidence: 99%

Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor

Phelps

Jaffe

Bradley

2013

Journal of Experimental Biology

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“…They are classified into two major groups: Activin/Nodal/TGF-β and BMP/GDF. TGF-β ligand binding induces the formation of a receptor complex that consists of receptor type II and type I, both of which are required for signal transduction (Luo and Lodish 1996;Renucci et al 1996;Lawler et al 1997;Garg et al 1999;Nagaso et al 1999). Both type II and type I receptors contain serine/threonine kinase domains in their intracellular portions.…”

Section: -1 Control Of Tgf-β β β β β Receptor Activationmentioning

confidence: 99%

“…Activin and Nodal have mesoderm-inducing abilities producing a variety of mesoderm derivatives in a dosedependent manner (Whitman 2001;Schier 2003). In zebrafish, the type I receptors consist of Activin receptor-like kinase (ALK)2 and ALK4, while type II receptors comprise ActRIIB (Luo and Lodish 1996;Renucci et al 1996;Lawler et al 1997;Garg et al 1999;Nagaso et al 1999). Nodal signaling can be promoted by phosphorylation of the transcription factor Smad2, which is mediated by Nodal/Activin/TGF-β doi:10.5047/absm.2010.00304.0111 © 2010 TERRAPUB, Tokyo.…”

Section: -1 Control Of Tgf-β β β β β Receptor Activationmentioning

confidence: 99%

“…During the activation process of tetrameric receptor complexes, the ActRIIB kinase acts as an upstream component of the type I receptor in the signaling pathways (Luo and Lodish 1996;Renucci et al 1996;Lawler et al 1997;Garg et al 1999;Nagaso et al 1999). Overexpression of ActRIIB kinase following the injection of cRNA into the zebrafish embryos induced the phosphorylation of Smad2.…”

Section: -1 Control Of Tgf-β β β β β Receptor Activationmentioning

confidence: 99%

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Stress Protein HSP70 in Fish

Yamashita¹,

Yabu²,

Ojima³

2010

Aqua-BioSci. Monogr. (ABSM)

127

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Stress proteins (heat-shock proteins, HSPs), which comprise an evolutionally wellconserved protein family, are induced in response to a variety of stress conditions and metabolic insults. When cells are subjected to sudden environmental changes, stress proteins are induced and play a central role in cellular homeostasis. A response to sudden adverse environmental changes is referred to as the heat-shock or stress response and is accompanied by a rapid increase in the synthesis of stress proteins. Given the importance of stress proteins in thermal adaptation at the cellular level, we have studied the expression, regulation, and protective functions of the members of the HSP70 stress protein family under normal and stress conditions in a variety of fish species. HSP70/heat-shock cognate protein-70 (HSC70) plays essential roles in the receptor complex formation and activation of Activin/Nodal/transforming growth factor-β and bone morphogenetic protein receptors and facilitates Nodal signaling. In addition, chaperone-mediated autophagy assisted by HSP70/heat-shock cognate (HSC)70 may be responsible for the stress responses in fish cells. HSP70 and HSC70 translocated into the lysosomes were found to accelerate protein degradation and catabolism under both stressed and normal conditions.

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Genes dependent on zebrafishcyclops function identified by AFLP differential gene expression screen

Rubinstein

Lee

Luo

et al. 2000

genesis

108

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Cloning of zebrafish activin type IIB receptor (ActRIIB) cDNA and mRNA expression of ActRIIB in embryos and adult tissues

Cited by 43 publications

References 59 publications

Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor

Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor

Stress Protein HSP70 in Fish

Genes dependent on zebrafishcyclops function identified by AFLP differential gene expression screen

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