No abstract

This study was the first to investigate the potential of AV biofeedback to improve the motion reproducibility of internal anatomy using MRI. The study demonstrated the significant improvement in diaphragm motion reproducibility using AV biofeedback combined with MRI. This system can potentially provide clinically beneficial motion management of internal anatomy in MRI and radiotherapy.

Preliminary results on phantom and patient cases confirm the feasibility of the proposed approach in an MRI-guided scenario, especially for non-rigid tumour motion compensation.

Through computer-generated, life-like digital landscapes, VR stands to change the current approach to pain management, medical training, neurocognitive diagnosis, and physical rehabilitation. Additional studies are needed to help define best practices in VR utilization, and to explore new therapeutic uses for VR in clinical practice.

Agrobacterium tumefaciens is a plant pathogen that has the natural ability of delivering and integrating a piece of its own DNA into plant genome. Although bacterial non-coding RNAs (ncRNAs) have been shown to regulate various biological processes including virulence, we have limited knowledge of how Agrobacterium ncRNAs regulate this unique inter-Kingdom gene transfer. Using whole transcriptome sequencing and an ncRNA search algorithm developed for this work, we identified 475 highly expressed candidate ncRNAs from A. tumefaciens C58, including 101 trans-encoded small RNAs (sRNAs), 354 antisense RNAs (asRNAs), 20 5′ untranslated region (UTR) leaders including a RNA thermosensor and 6 riboswitches. Moreover, transcription start site (TSS) mapping analysis revealed that about 51% of the mapped mRNAs have 5′ UTRs longer than 60 nt, suggesting that numerous cis-acting regulatory elements might be encoded in the A. tumefaciens genome. Eighteen asRNAs were found on the complementary strands of virA, virB, virC, virD, and virE operons. Fifteen ncRNAs were induced and 7 were suppressed by the Agrobacterium virulence (vir) gene inducer acetosyringone (AS), a phenolic compound secreted by the plants. Interestingly, fourteen of the AS-induced ncRNAs have putative vir box sequences in the upstream regions. We experimentally validated expression of 36 ncRNAs using Northern blot and Rapid Amplification of cDNA Ends analyses. We show functional relevance of two 5′ UTR elements: a RNA thermonsensor (C1_109596F) that may regulate translation of the major cold shock protein cspA, and a thi-box riboswitch (C1_2541934R) that may transcriptionally regulate a thiamine biosynthesis operon, thiCOGG. Further studies on ncRNAs functions in this bacterium may provide insights and strategies that can be used to better manage pathogenic bacteria for plants and to improve Agrobacterum-mediated plant transformation.

Aquatic species throughout the interior Columbia River basin are at risk. Evaluation of the potential effects of federal land management on aquatic ecosystems across this region is an important but challenging task. Issues include the size and complexity of the systems, uncertainty in important processes and existing states,¯exibility and consistency in the analytical framework, and an ability to quantify results. We focused on salmonid ®shes and their habitats as indicators of conditions in aquatic ecosystems and used Bayesian belief networks as a formal, quantitative framework to address the issues in our evaluation of land management alternatives proposed for the interior Columbia River basin. Because empirical information is limited at the scales relevant to our analysis, an ability to combine both empirical and more subjective information was key to the analysis. The representation of linkages through conditional probabilities made uncertainty explicit. We constructed two general networks. One represented the in¯uence of landscape characteristics and existing and predicted management activities on aquatic habitats. A second represented the in¯uence of habitat, existing biotic conditions, and for two anadromous species, ocean and migratory conditions, on the status of six widely distributed salmonid ®shes. In the long term (100 years) all three land management alternatives were expected to produce positive changes in the status and distribution of the salmonids and their habitats. Trends were stronger for habitat than for the status of salmonids because of greater uncertainty in linking the ®sh and habitat networks and constraints outside spawning and rearing habitat on federal lands in the study area. Trends were stronger for resident salmonids than anadromous forms because of additional effects of the migratory corridor assumed for the latter. Alternative S2, which approached ecosystem restoration more conservatively, generally produced the strongest positive changes, and alternative S3, designed to promote more aggressive restoration, the weakest. Averaged across the basin, differences among the alternatives were small. Differences were greater at ®ner temporal and spatial scales. In the short term (10 years) alternative S3 was expected to lead to further degradation in some areas. By formalizing our understanding and assumptions in these networks, we provided a framework for exploring differences in the management alternatives that is more quanti®able, spatially explicit, and¯exible than previous approaches. Published by Elsevier Science B.V.

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These results demonstrated that AV biofeedback significantly improved intra- and interfraction lung tumor motion consistency for lung cancer patients. These results demonstrate that AV biofeedback can facilitate consistent tumor motion, which is advantageous toward achieving more accurate medical imaging and radiation therapy procedures.