Cloning of the goat β-casein-encoding gene and expression in transgenic mice

Roberts, Bruce; DiTullio, Paul; Vitale, James; Hehir, Kathleen; Gordon, Katherine

doi:10.1016/0378-1119(92)90129-d

Cited by 105 publications

(70 citation statements)

References 30 publications

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“…In the rabbit (36), goat (26), and bovine (3) ,B-casein promoters, the corresponding sequence TCA'lTITTCT also binds YY1, albeit with lower affinity. The consensus binding site of YY1 (also called CF-1, b, or NF-El) on different promoters consists of the core CCATnT (25).…”

Section: Resultsmentioning

confidence: 99%

The nuclear factor YY1 participates in repression of the beta-casein gene promoter in mammary epithelial cells and is counteracted by mammary gland factor during lactogenic hormone induction.

Meier¹,

Groner²

1994

Mol. Cell. Biol.

135

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(29,30). MGF binding activity is regulated in vivo and in vitro. High levels were found in nuclear extracts derived from mammary cells of lactating mice and in HC11 cells induced with lactogenic hormones. The regulation of the DNA binding activity of MGF is at least in part due to its state of phosphorylation (30,31).The introduction of mutations into the promoter sequence and gene transfer into HC11 cells revealed the presence of additional regulatory elements in the 1-casein gene promoter. A negatively acting element was found in the region adjacent to the MGF binding site. This element located between -110 and -150 seems to be constituted by two interacting half sites. Mutations in the distal half site (-135 to -145) or in the proximal half site (-110 to -120) by themselves had little effect on the rate of transcription. The simultaneous mutations of both sites resulted in a high level of hormone-independent transcription. Two specific DNAprotein complexes were formed when DNA from this region was used as a probe in band shift experiments. These complexes were down-regulated in extracts of induced cells.These observations led us to propose that one mechanism by which hormones regulate ,-casein transcription is the relief of repression (29).We have further investigated the DNA-protein complexes formed by this promoter region and found that a novel nuclear factor binds to the proximal half of the negative 128 on March 24, 2019 by guest

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Section: Resultsmentioning

confidence: 99%

The nuclear factor YY1 participates in repression of the beta-casein gene promoter in mammary epithelial cells and is counteracted by mammary gland factor during lactogenic hormone induction.

Meier¹,

Groner²

1994

Mol. Cell. Biol.

135

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“…The exon-intron boundary of the human CSN1 gene followed the GT/AG rule and was highly homologous with the consensus sequences [12]. Human CSN1 and CSN2 genes sequences characterized in this study show high homology with those of other mammalian species, including cattle [11,13], goats [14], mice [15], rabbits [16,17], rats [18,19] and sheep [20] and possess 60-71% and 64-73% homology with those of other mammalian species. Furthermore, the nucleotide sequences of the regions between positions -200 and -1 of human CSN1 and CSN2 genes are highly conserved with those of other mammalian casein genes.…”

Section: Resultsmentioning

confidence: 64%

A Comparative Study of the 5' Flanking Sequences of the .ALPHA.S1- and .BETA.-Casein Genes: Human and Other Mammalian Species.

Fujiwara¹,

Miwa²,

Suzuki³

et al. 1997

Journal of Reproduction and Development

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Abstract. The 5' flanking region (1.2 kb) and the first exon and intron (1.5 kb) of the human αS1-casein (CSN1) gene, and the 5' flanking sequence (2.0 kb) of the human β-casein (CSN2) gene were cloned and sequenced. Strong sequence homology was observed between the 5' flanking regions of the casein genes of humans and other mammals. Several transcriptional regulatory elements (GRE, C/EBP, YY1, STAT5) identified in the rat β-casein gene were conserved in both human casein genes.

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“…In this study, the LR gene is under the control of the goat -casein promoter (Roberts et al, 1992;Persuy et al, 1995) which is one of the most abundant proteins in the milk, and included in the construct to direct the efficient gene expression in milk-specific way at lactating stage. Other studies have shown that the goat -casein promoter can efficiently direct the high expression of a foreign gene in the milk of transgenic animals, such as human serum albumin (up to 40 g/L) in bovine milk (Echelard et al, 2009) lysozyme (up to 1.45 g/L) in transgenic mice (Yu et al, 2006) and lactoferrin ( 2.5 g / L and 3.4 g / L) in two transgenic cows .…”

Section: Construction Of a Recombinant Lr-his Tag Gene Expression Vecmentioning

confidence: 99%

Expression and characterization of a lysine rich protein in cow milk

Zhang

et al. 2018

IJAR

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The lysine is considered as the most important essential amino acid, because it is the most limiting in the cereals grains. In this study, a lysine-rich (LR) gene, and the expression vector pcDNA3.1-LR and pBC1-LR were constructed. The LR was expressed in 293T cells driven by the vector pcDNA3.1-LR and checked by RT-PCR and WB. The mammary gland tissuespecific expression vector carrying the LR was injected directly into the lactating mammary glands of cows and the milk samples were checked by a complete amino acid analysis. The results showed that the LR protein was expressed successfully in cells and in cow milk; the expression of LR lasted for 6 d, and the lysine level of the injection group was significantly higher than that of negative controls (p <0.05). This study provide a better understanding of how mammary gland expression systems increase the lysine content of milk that can be applied to transgenic dairy cow.

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Cloning of the goat β-casein-encoding gene and expression in transgenic mice

Cited by 105 publications

References 30 publications

The nuclear factor YY1 participates in repression of the beta-casein gene promoter in mammary epithelial cells and is counteracted by mammary gland factor during lactogenic hormone induction.

The nuclear factor YY1 participates in repression of the beta-casein gene promoter in mammary epithelial cells and is counteracted by mammary gland factor during lactogenic hormone induction.

A Comparative Study of the 5' Flanking Sequences of the .ALPHA.S1- and .BETA.-Casein Genes: Human and Other Mammalian Species.

Expression and characterization of a lysine rich protein in cow milk

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