Cloning of a xylanase gene xyn2A from rumen fungus Neocallimastix sp. GMLF2 in Escherichia coli and its partial characterization

Akyol, İsmail; Çömlekçioğlu, Uğur; Kar, Bülent; Ekinci, Mehmet Sait; Özköse, Emin

doi:10.2478/s11756-009-0140-5

Cited by 6 publications

(2 citation statements)

References 38 publications

(32 reference statements)

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“…The genes encoding xylanases from Neocallimastix sp. GMLF2 [66], Orpinomyces sp. Strain 2, and Neocallimastix frontalis [67] were also cloned into E. coli, and a high expression was obtained.…”

Section: Digestion By Diverse Plant Fiber-degrading Enzymesmentioning

confidence: 99%

Hydrogenosome, Pairing Anaerobic Fungi and H2-Utilizing Microorganisms Based on Metabolic Ties to Facilitate Biomass Utilization

Zhong

et al. 2022

JoF

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Anaerobic fungi, though low in abundance in rumen, play an important role in the degradation of forage for herbivores. When only anaerobic fungi exist in the fermentation system, the continuous accumulation of metabolites (e.g., hydrogen (H2) and formate) generated from their special metabolic organelles—the hydrogenosome—inhibits the enzymatic reactions in the hydrogenosome and reduces the activity of the anaerobic fungi. However, due to interspecific H2 transfer, H2 produced by the hydrogenosome can be used by other microorganisms to form valued bioproducts. This symbiotic interaction between anaerobic fungi and other microorganisms can be used to improve the nutritional value of animal feeds and produce value-added products that are normally in low concentrations in the fermentation system. Because of the important role in the generation and further utilization of H2, the study of the hydrogensome is increasingly becoming an important part of the development of anaerobic fungi as model organisms that can effectively improve the utilization value of roughage. Here, we summarize and discuss the classification and the process of biomass degradation of anaerobic fungi and the metabolism and function of anaerobic fungal hydrogensome, with a focus on the potential role of the hydrogensome in the efficient utilization of biomass.

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Section: Digestion By Diverse Plant Fiber-degrading Enzymesmentioning

confidence: 99%

Hydrogenosome, Pairing Anaerobic Fungi and H2-Utilizing Microorganisms Based on Metabolic Ties to Facilitate Biomass Utilization

Zhong

et al. 2022

JoF

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“…Bacteria have many advantages compared to other microorganisms such as requiring less growth time.Also, bacterial xylanase is more functioning at high temperature and alkaline conditions, which are more appropriate for most applications (Subramaniyan et al, 2001). Some xylanase genes have been cloned and expressed in E.coli (Akyol et al, 2009;Guo et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cloning of the xylanase gene from soil Streptomyces into Escherichia coli for the poultry industry application

BAHRAMINIA

MORADMAND

Amini

2022

J Hellenic Vet Med Soc

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Streptomyces are gram-positive aerobic strains that are isolated from soil, water, sediments, and various sources. The bacteria are capable of producing secondary metabolites, such as enzymes that sometimes play unique functional roles in industry, and are one of the important bio-control agents. This study aimed to isolate and clone thexylanase gene from soil Streptomyces. Soil samples were collected from Markazi Province, Iranafter specific biochemical examinations, isolation of bacteria, and DNA extraction. PCR was then performed to identify the strains containing the xylanase gene. The gene from the positive strains was cloned into an E.coli host-vector by TA cloning technique and finally, the expression of genes in E. coli origami was measured by Real-Time PCR technique. ClustalX and Mega5 software were used to draw the phylogenetic tree. A total of twelve Streptomyces isolates were identified from the soil samples. Among all the isolates, threeones had the xylanase gene. After cloning the xylanase genes, the cloned strains were isolated. To confirm the DNA cloning, Real-Time PCR was performed, and finally, the PCR product where sequenced. In this study, Streptomyces was identified as a native strain for the expression of xylanase after generating recombinant plasmid and TA cloning. It can be stated that cloning of the xylanase gene from soil Streptomyces in E. coli can be used in the poultry industry.

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GH11 xylanases: Structure/function/properties relationships and applications

Paës¹,

Berrin²,

Beaugrand³

2012

Biotechnology Advances

376

283

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Cloning of a xylanase gene xyn2A from rumen fungus Neocallimastix sp. GMLF2 in Escherichia coli and its partial characterization

Cited by 6 publications

References 38 publications

Hydrogenosome, Pairing Anaerobic Fungi and H2-Utilizing Microorganisms Based on Metabolic Ties to Facilitate Biomass Utilization

Hydrogenosome, Pairing Anaerobic Fungi and H2-Utilizing Microorganisms Based on Metabolic Ties to Facilitate Biomass Utilization

Cloning of the xylanase gene from soil Streptomyces into Escherichia coli for the poultry industry application

GH11 xylanases: Structure/function/properties relationships and applications

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