Cloning of a recA-like gene of Proteus mirabilis

Eitner, G.; Solonin, Alexander S.; Tanyashin, V. I.

doi:10.1016/0378-1119(81)90162-1

Cited by 25 publications

(5 citation statements)

References 19 publications

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“…Furthermore, restriction mapping ( Fig. la) was consistent with the previously published map (Eitner et al, 1981) and with the data obtained by sequencing (Akaboshi et al 1989). …”

Section: Cloning Of the Reca Genes Of Serratia Marcescens And Pro Teusupporting

confidence: 90%

“…A genomic library of P . mirabilis PG 1300 (Eitner et al, 1981) was constructed with chromosomal DNA fragmented with Sau3AI to a size of 10-15 kb and cloned into the BamHI site of pBR322 (Bolivar et al 1977). Screening of clones on mitomycin C medium (see previous paragraph) identified one plasmid from which, after a partial Sau3AI digest, a 4.2 kb fragment covering recA was cloned into the BamHI site of the mini-Fderived single-copy vector pJE256 (Table 1, Fig.…”

Section: Cloning Of the Reca Genes Of Serratia Marcescens And Pro Teumentioning

confidence: 99%

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Recombination and UV resistance of Escherichia coli with the cloned recA and recBCD genes of Serratia marcescens and Proteus mirabilis: evidence for an advantage of intraspecies combination of P. mirabilis RecA protein and RecBCD enzyme

Vries

Wackernagel²

1992

Journal of General Microbiology

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In Escherichia coii, constituents of the main recombination pathway are provided by the genes recA (RecA protein) and recBCD (RecBCD enzyme). Recombination in conjugation experiments and repair of UV damage of E. coii mutants deleted for recA, for recBCD or for recA plus recBCD were restored, although to different degrees, by the cloned recA and recBCD genes from Serratia marcescens or Proteus mirabiiis. When both recombination enzymes were from the same species, repair and recombination efficiencies had the order E. coii > S. marcescens > P. mirabiiis. However, the P. mirabilis recA plus recBCD genes resulted in higher levels of repair and recombination than those obtained with one component from P. mirabiiis ( r e d or recBCD) and the other from E. coii or S. marcescens. The data provide evidence for the similarity of RecABCD pathways of recombination among enteric bacteria and suggest an in oioo advantage of an intraspecies combination of P. mirabiiis RecA protein and RecBCD enzyme over interspecies combinations. This could point to a cooperation between these basic recombination enzymes. The molecular processes which could be involved are discussed.

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“…Furthermore, restriction mapping ( Fig. la) was consistent with the previously published map (Eitner et al, 1981) and with the data obtained by sequencing (Akaboshi et al 1989). …”

Section: Cloning Of the Reca Genes Of Serratia Marcescens And Pro Teusupporting

confidence: 90%

Section: Cloning Of the Reca Genes Of Serratia Marcescens And Pro Teumentioning

confidence: 99%

Recombination and UV resistance of Escherichia coli with the cloned recA and recBCD genes of Serratia marcescens and Proteus mirabilis: evidence for an advantage of intraspecies combination of P. mirabilis RecA protein and RecBCD enzyme

Vries

Wackernagel²

1992

Journal of General Microbiology

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“…Initial attempts to obtain the cyanobacterial recA gene by selecting for the acquisition of UV resistance resulted in the recovery of a number of Ampr UVr clones; however, plasmid could not be isolated from these cells. The reasons for this result are unclear, as this selection technique has been used for the isolation of the recA gene from Proteus mirabilis (5). These results suggest that the repair of UV irradiation damage has led to, or required, the integration of the A.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis

Owttrim

Coleman

1987

J Bacteriol

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A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as recombination proficiency when measured by Hfr-mediated conjugation. The cyanobacterial recA gene restored spontaneous but not mitomycin C-induced prophage production. Restriction analysis and subcloning yielded a 1.5-kilobase-pair Sau3A fragment which also restored methylmethane sulfonate resistance and coded for a 38- to 40-kilodalton polypeptide when expressed in an in vitro transcription-translation system.

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“…Thus, the recA gene in E. coli is a multifunctional enzyme which plays a central role in various DNA metabolic pathways, including the catalysis of homol-little is known about DNA metabolism in other bacterial species. Gene products with activities similar to the recA protein of E. coli have been identified in several other species, including Bacillus subtilis (13), Proteus mirabilis (15), Shigella flexneri (33), Rhizobium meliloti (2), Erwinia carotovora (33), Neisseria gonorrhoeae (35), Vibrio cholerae (21,27), Pseudomonas syringae (29), and Pseudomonas aeruginosa (44). While immunochemical studies have shown the presence of conserved functional domains among many of these recA proteins, measurable homology in their DNA coding sequences was not always detectable by hybridization techniques (27,33).…”

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confidence: 99%

Transcriptional and translational analyses of recA mutant alleles in Pseudomonas aeruginosa

Horn

Ohman

1988

J Bacteriol

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Recombinant plasmids containing the recA gene from Pseudomonas aeruginosa were used in complementation, transcriptional, and translational studies to examine the nature of rec-102 and rec-2, mutations which confer a recA-like mutant phenotype on P. aeruginosa PAO strains. For comparison, recA7::TnSO0 mutants of strain PAO were constructed by gene replacement. The rec-2 and rec-102 alleles were shown to be recA alleles; plasmids containing the recA gene complemented the three rec mutant strains for defects associated with recA mutation. Northern blot analyses indicated that the recA gene in P. aeruginosa was transcribed as two distinct mRNAs of approximately 1.2 and 1.4 kilobases (kb). A plasmid encoding both transcripts of recA complemented all defects associated with the three recA mutations rec-2, rec-102, and recA7. However, a 2.

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Cloning of a recA-like gene of Proteus mirabilis

Cited by 25 publications

References 19 publications

Recombination and UV resistance of Escherichia coli with the cloned recA and recBCD genes of Serratia marcescens and Proteus mirabilis: evidence for an advantage of intraspecies combination of P. mirabilis RecA protein and RecBCD enzyme

Recombination and UV resistance of Escherichia coli with the cloned recA and recBCD genes of Serratia marcescens and Proteus mirabilis: evidence for an advantage of intraspecies combination of P. mirabilis RecA protein and RecBCD enzyme

Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis

Transcriptional and translational analyses of recA mutant alleles in Pseudomonas aeruginosa

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