Cloning of a developmentally amplified gene sequence in the DNA puff C4 of Bradysia hygida (Diptera: Sciaridae) salivary glands

A 28-fold increase in secretory protein synthesis is associated with DNA puff activity in the salivary gland of Bradysia hygida (Diptera, Sciaridae)

“…This is exactly what we have here: at least for DNA puffs C4 and B10 the extent of amplification was established for DNA sequences that code for mRNA to be about 22 and 10 times, respectively (6)(7)(8)(9). A large increase in protein production and secretion was detected at the time the DNA puffs were active, with a peak of about 28 times at age E7+6h (Figure 1).…”

“…A similar level of amplification was found in the puff II/9 of Sciara coprophila (5). The extent of amplification of the DNA puffs C4 J.C. de-Almeida and B10 of Bradysia hygida was established to be about 22 and 10 times, respectively, for DNA sequences that code for mRNA (6)(7)(8)(9). There is evidence indicating that DNA puff activity is involved in the production of specific proteins in the salivary glands of Rhynchosciara americana (10)(11)(12)(13), Bradysia hygida (14) and Trichosia pubescens (15), some of them being secretory proteins (9,11,12,16,17).…”

Functional and bioinformatics analyses reveal conservation of cis‐regulatory elements between sciaridae and drosophilidae

“…The BhC4-1 DNA puff gene of Bradysia hygida is amplified 21-fold and abundantly transcribed in the salivary gland when DNA puff C4 is formed at the end of the fourth larval instar (Basso et al, 2002;Paçó-Larson et al, 1992). DNA puff C4 formation and the amplification and expression of the BhC4-1 gene are all induced as a late response to the increase of ecdysone titers that triggers metamorphosis (Basso et al, 2002).…”

“…These genomic regions constitute sites of developmentally regulated gene amplification and transcription (Basso et al, 2002;Foulk et al, 2006;Gerbi et al, 1993;Lara et al, 1991;Stocker et al, 1996). The formation of DNA puffs is induced by ecdysone and is concomitant with the transcription of the genes which present the highest levels of amplification within the amplicons (Fontes et al, 1992;Gerbi et al, 1993;Glover et al, 1982;Paçó-Larson et al, 1992;Penalva et al, 1997;Santelli et al, 1991Santelli et al, , 2004Wu et al, 1993;Yokosawa et al, 1999).…”

Bradysia hygida (Diptera, Sciaridae) presents two eukaryotic Elongation Factor 1A gene homologues: partial characterization of the eukaryotic Elongation Factor 1A-F1 gene

“…Taken together, these data further support the existence of two paralogous copies of the eEF1A gene in the nematoceran Dipteran B. hygida. Sciarid DNA puffs have been previously characterized as genomic regions in which developmentally regulated gene amplification occurs (33)(34)(35)(36)(37)(38). The only DNA puff-forming regions found on chromosomes A and X constitute the A14 and X4 regions, respectively (6).…”