Cloning of a defective polyoma DNA molecule with rearrangements in the region coding for middle and large T antigens

Parent, Carmen; Bastin, Marcel

doi:10.1016/0014-5793(81)81136-2

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…2. To supplement the middle T gene with the region encoding the N-terminal 25% of the large T polypeptide, we cloned a Py virusdefective molecule, pHdfS5 (13), in the HindIll site of pMT3. The defective molecule (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Role of the Three Polyoma Virus Early Proteins in Tumorigenesis

1983

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Section: Resultsmentioning

confidence: 99%

“…This molecule was chosen for several reasons. First, it is unable to transform cells in vitro (13) and does not induce tumors in newborn rats (Table 1). Second, the existence of a possible polyadenylation signal at 25.7 m.u.…”

Section: Resultsmentioning

confidence: 99%

Role of the Three Polyoma Virus Early Proteins in Tumorigenesis

1983

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Tumorigenic activity of polyoma virus and SV40 DNAs in newborn rodents

et al. 1984

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Role of the Three Polyoma Virus Early Proteins in Tumorigenesis

Asselin

Gélinas

Bastin

1983

Molecular and Cellular Biology

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A modified polyoma virus genome which can encode the middle T protein but not the large or small T proteins transforms rat cells in culture with an efficiency about 20% that of the wild-type genome. Although middle T-transformed cells grow as tumors when transplanted into nude mice or syngeneic rats, the middle T gene alone is totally inactive when used in a more stringent and rigorous assay for tumorigenicity such as the injection of DNA into newborn rats. Thus, functions other than those expressed by middle T antigen are required for the elaboration of all the properties associated with tumorigenesis. To assess whether a complementary function could be exerted by the large or the small T antigen, we constructed plasmids containing two modified early regions which independently encoded middle T and one of the two other proteins. Both recombinants were tumorigenic in newborn rats. Cell lines derived by transfer of these plasmids under no special selective conditions did not acquire the property of growth in low-serum medium but exhibited the same tumorigenic properties as wild-type polyoma DNA-transformed cells. Furthermore, a recombinant which encoded the middle and small T antigens, but not the large T antigen, was tumorigenic in newborn rats. Although the small T antigen provides a complementary function for tumorigenicity, it cannot complement the middle T antigen for an efficient induction of transformation of cultured cells. This suggests that the complementary function exerted by the small T antigen is different from that of the N-terminal fragment of the large T protein.

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Cloning of a defective polyoma DNA molecule with rearrangements in the region coding for middle and large T antigens

Cited by 3 publications

References 14 publications

Role of the Three Polyoma Virus Early Proteins in Tumorigenesis

Role of the Three Polyoma Virus Early Proteins in Tumorigenesis

Tumorigenic activity of polyoma virus and SV40 DNAs in newborn rodents

Role of the Three Polyoma Virus Early Proteins in Tumorigenesis

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