Cloning, in vitro expression and bioactivity of duck interleukin-18

Chen, Hongying; Bao-an, Cui; Xia, Pingan; Li, Xinsheng; Hu, Gong-Zheng; Yang, Mingfan; Zhang, Hongying; Wang, Xuebin; Sufang, Cao; Zhang, Longxian; Kang, Xiangtao; Ke, Tu

doi:10.1016/j.vetimm.2008.01.036

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…The expression of cytokines has be(>n demonstrated in peripheral blood or spleen nionormclear cells in ducklings in vitro (Wu et al, 2007;Chen et al, 2008;Wu et al,, 2008), Our work is the first report on the cytokine repertoire in duckling livers in the context of DHV infection. In the present study, we examined several cytokines, namely IL-lß, IL-6, IFN-a, TNF-a, and IL-10, The IL-lß and IL-6, which are classified as proinflammatory cytokines, are involved in the activation of B and T cells and the development of macrophages in chickens (Wigley and Kaiser, 2003;Giansanti et al, 2006).…”

Section: Discussionmentioning

confidence: 58%

“…It has been reported that the amount of the transcripts for cytokines was examined in the early days of infection of Salmonella Typhimurium (Sijben et al, 2003;Degen et al, 2005), Eimeria maxima (Hong et al, 2006), and Marek's disease virus Parvizi et al, 2009) in chickens. Through current developments in molecular biology, many immtinerelated genes of ducks have been cloned and expressed 583 in vitro (Wu et al, 2007;Chen et al, 2008;Wu et al, 2008). Yang et al (2008a) reported that high levels of persistent IFN-a mRNA expression in the liver could be induced using the attenuated duck plague virus.…”

Section: Introductionmentioning

confidence: 99%

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Cytokine gene expression in the livers of ducklings infected with duck hepatitis virus-1 JX strain

Gu¹,

Xie

Hu³

et al. 2012

Poultry Science

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Duck hepatitis virus type 1 (DHV-1) causes a highly contagious disease in ducklings and is often associated with liver necrosis, hemorrhages, and high mortality. In the current study, the expression levels of gene transcripts encoding proinflammatory cytokines and the virus were measured by quantitative reverse-transcription PCR in duck livers after infection with a DHV-1 JX isolate obtained from natural cases in Hubei Province, China. In addition, sera IL-1β, IL-6, and alanine aminotransferase levels were quantified. Liver histopathology was examined following DHV-1 infection. The ducklings died within 1 to 2 d postinfection (d.p.i.) because of typical liver degeneration, hemorrhage, necrosis, and bile-duct epithelial cell proliferation. Transcripts of the cytokines IFN-α, IL-6, TNF-α, and IL-10 decreased by 0.5 d.p.i. and then gradually increased at 1 d.p.i. Similarly, DHV-1 JX 3D gene levels in the liver sharply increased at 1 d.p.i. and then maintained a high level. In contrast, liver TNF-α and IL-1β transcripts showed no increased expression of the cytokine gene postinfection and significantly decreased compared with the expression at 0.25 d.p.i., only the expression of IFN-α transcripts increased 128-fold by 1 d.p.i. Changes in the serum IL-6 level remained relatively stable postinfection and not significantly different compared with that of the control (P > 0.05), whereas serum levels of IL-1β significantly decreased at 0.5 d.p.i. and increased from 1 d.p.i. onwards (P < 0.05). Serum alanine aminotransferase levels significantly increased 2 d.p.i. compared with that of the control group (P < 0.01), which seemed to keep with the number of dead ducks. The cytokines exhibited a biphasic pattern following DHV-1 JX infection. Taken together, the data indicated that duckling liver inflammatory responses were produced following experimental DHV-1 JX infection involving multiple cytokines.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Cytokine gene expression in the livers of ducklings infected with duck hepatitis virus-1 JX strain

Gu¹,

Xie

Hu³

et al. 2012

Poultry Science

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“…Splenic mononuclear cells (SMC) were isolated from the spleens of 3‐month‐old HN pigs that were obtained from the Zhengzhou slaughter house, as described previously (Chen et al , 2008). The gene encoding pIL‐18 was amplified from spleen cells using RT‐PCR.…”

Section: Methodsmentioning

confidence: 99%

Cloning,in vitroexpression, and bioactivity of interleukin-18 isolated from a domestic porcine breed found in Henan

Chen

Zheng

et al. 2009

FEMS Immunology &Amp; Medical Microbiology

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To evaluate the effects of recombinant porcine interleukin-18 (rpIL-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine IL-18 (pIL-18) isolated from a domestic big-white porcine breed found in the Henan province (HN) was cloned using a reverse transcriptase-PCR. The cloned HN pIL-18 contained an ORF of 579 base pairs encoding a 192-amino-acid precursor protein. The amino acid sequence of HN pIL-18 was compared with all the other pIL-18 amino acid sequences and varied by at least one amino acid to the consensus of all the others available. HN pIL-18 mature protein gene was inserted into a prokaryotic vector pGEX-4T-1 and expressed in Escherichia coli BL21. The expression of glutathione-S-transferase-pIL18 fusion protein was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. The rpIL-18 induced in vitro proliferation of concanavalin-A-stimulated porcine splenocytes, as revealed by the MTT assay. We studied the antiviral activities of the rpIL-18 on the replication of porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), and porcine parvovirus (PPV) cultured in two homologous cell lines. The results suggested that rpIL-18 can stimulate the proliferation of lymphocytes and inhibit viral pathogens infecting the porcine population.

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“…Recombinant duIL-18 showed bioactivity in the lymphocyte proliferation assay, and both mAb and polyclonal Ab antibodies were able to block proliferation of ConA-stimulated splenic mononuclear cells [74]. Recombinant duIL-18 showed bioactivity in the lymphocyte proliferation assay, and both mAb and polyclonal Ab antibodies were able to block proliferation of ConA-stimulated splenic mononuclear cells [74].…”

Section: Interleukin-18mentioning

confidence: 99%