Cloning in cattle: from embryo splitting to somatic nuclear transfer

Heyman, Yvan; Vignon, Xavier; Chesné, P.; Bourhis, Daniel Le; Marchal, Jacques; Renard, Jean‐Paul

doi:10.1051/rnd:19980602

Cited by 38 publications

(12 citation statements)

References 22 publications

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“…2A-D). Our results share similar conclusions with studies on the generation of identical animals (Heyman et al 1998, Chan et al 2000, Mitalipov et al 2002, Schramm & Paprocki 2004 and the generation of an ES cell from a single BTM (Mitalipov et al 2002, Wakayama et al 2007, in that sister BTMs are in general not equally competent at establishing ES cell lines or the generation of identical offspring. However, the capability of sister BTMs in complementing the lost or damaged BTM of an embryo could not be determined.…”

Section: Es Cells From Single Blastomere-derived Embryossupporting

confidence: 90%

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Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Lorthongpanich¹,

Yang²,

Piotrowska-Nitsche³

et al. 2008

Reproduction

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The recently developed technique of establishing embryonic stem (ES) cell lines from single blastomeres (BTMs) of early mouse and human embryos has created significant interest in this source of ES cells. However, sister BTMs of an early embryo might not have equal competence for the development of different lineages or the derivation of ES cells. Therefore, single BTMs from two-and four-cell embryos of outbred mice were individually placed in sequential cultures to enhance the formation of the inner cell mass (ICM) and the establishment of embryonic outgrowth. The outgrowths were then used for the derivation of ES cell lines. Based on the expression of ICM (Sox2 ) and trophectoderm (Cdx2 ) markers, it was determined that ICM marker was lacking in blastocysts derived from 12% of BTMs from two-cell stage and 20% from four-cell stage. Four ES cell lines (5.6%; 4/72) were established ater culture of single BTMs from twocell embryos, and their pluripotency was demonstrated by their differentiation into neuronal cell types. Our results demonstrate that sister BTMs of an early embryo are not equally competent for ICM marker expression. However, we demonstrated the feasibility of establishing ES cells from a single BTM of outbred mice. Reproduction (2008) 135 805-813

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Section: Es Cells From Single Blastomere-derived Embryossupporting

confidence: 90%

“…Recent studies on embryo splitting have further demonstrated the pluripotency of embryonic BTMs. Unfortunately,not all BTMs were able to develop to term (Heyman et al 1998, Chan et al 2000, Mitalipov et al 2002. On the contrary, two-, four-, and eight-cell sheep embryos show no difference in producing monozygotic twins (Willadsen 1979).…”

Section: Introductionmentioning

confidence: 98%

Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Lorthongpanich¹,

Yang²,

Piotrowska-Nitsche³

et al. 2008

Reproduction

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“…Although twin bovine [6][7][8], ovine [9], caprine [10,11], equine [12], porcine [13], and rhesus monkey [14] young have been created by dividing multicellular, preimplantation embryos into two halves, producing twin offspring from single blastomeres appears to have been more challenging, with only a few cases reported [15,16]. Possibly because of the availability of inbred strains, less attention has been paid to creating twins in the mouse than in commercially important farm species such as cattle, although twin production by bisection of murine morulae has been successful [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Development of Monozygotic Twin Mouse Embryos from the Time of Blastomere Separation at the Two-Cell Stage to Blastocyst1

Katayama

Ellersieck

Roberts

2010

Biology of Reproduction

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The development of blastomeres separated from two-cell stage murine embryos has been compared. Blastomeres were removed from the zona pellucida (ZP) and cultured individually; the twin embryos were compared during their progression to blastocyst in terms of development rate, cell number, morphology, conformation at the four-cell stage, and CDX2 and POU5F1 (also known as OCT4) expression. In general, twin embryos, whether obtained from superovulated or normally bred dams, displayed comparable cell numbers as they advanced. They formed morulae and blastocysts more or less synchronously with each other and with control embryos, although possessing about half of the latter's cell number. Despite this apparent synchrony, the majority of twin blastocysts differed in terms of their relative complements of POU5F1+/CDX2- cells, which represent inner cell mass (ICM), and POU5F1+/CDX2+ cells, which identify trophectoderm (TE). Many, but not all, exhibited a disproportionately small ICM. By contrast, demiembryos retained within their ZP and created by randomly damaging one of the two blastomeres in two-cell stage embryos exhibited a more normal ratio of ICM to TE cells at blastocyst and significantly less variance in ICM cell number. One possible explanation is that ZP-free demiembryos only infrequently adopt the same conformation as their partners, including the favorable tetrahedral form, at the four-cell stage, suggesting that such embryos exhibit a high degree of plasticity with regard to the orientation of their first two cleavage planes and that a significant number likely deviate from paths that provide an optimal geometric progression to blastocyst. These data could explain the difficulty of creating monozygotic twins from two-cell stage embryos.

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“…Similar cloning efficiency was noted when the nuclear transfer technique was applied to a variety of somatic cells (Tsunoda & Kato, 2000). The success rate of nuclear transfer technique to generate live births is only 1-2% (Heyman, et al, 1998; Hiiragi & Solter, 2005; Rhind, et al, 2003; Wakayama, et al, 2000)…”

Section: Techniques To Generate Pluripotent Stem Cellsmentioning

confidence: 99%

Advances in Reprogramming Somatic Cells to Induced Pluripotent Stem Cells

Patel

Yang

2010

Stem Cell Rev and Rep

182

128

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Traditionally, nuclear reprogramming of cells has been performed by transferring somatic cell nuclei into oocytes, by combining somatic and pluripotent cells together through cell fusion and through genetic integration of factors through somatic cell chromatin. All of these techniques changes gene expression which further leads to a change in cell fate. Here we discuss recent advances in generating induced pluripotent stem cells, different reprogramming methods and clinical applications of iPS cells.Viral vectors have been used to transfer transcription factors (Oct4, Sox2, c-myc, Klf4, and nanog) to induce reprogramming of mouse fibroblasts, neural stem cells, neural progenitor cells, keratinocytes, B lymphocytes and meningeal membrane cells towards pluripotency. Human fibroblasts, neural cells, blood and keratinocytes have also been reprogrammed towards pluripotency. In this review we have discussed the use of viral vectors for reprogramming both animal and human stem cells. Currently, many studies are also involved in finding alternatives to using viral vectors carrying transcription factors for reprogramming cells. These include using plasmid transfection, piggyback transposon system and piggyback transposon system combined with a non viral vector system. Applications of these techniques have been discussed in detail including its advantages and disadvantages. Finally, current clinical applications of induced pluripotent stem cells and its limitations have also been reviewed. Thus, this review is a summary of current research advances in reprogramming cells into induced pluripotent stem cells.

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Cloning in cattle: from embryo splitting to somatic nuclear transfer

Cited by 38 publications

References 22 publications

Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Development of Monozygotic Twin Mouse Embryos from the Time of Blastomere Separation at the Two-Cell Stage to Blastocyst1

Advances in Reprogramming Somatic Cells to Induced Pluripotent Stem Cells

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