Cloning, identification and accurate normalization expression analysis of PPARα gene by GeNorm in Megalobrama amblycephala

Zhao, Ying‐Zheng; Gul, Yasmeen; Su, Li; Wang, Weimin

doi:10.1016/j.fsi.2011.06.024

Cited by 62 publications

(20 citation statements)

References 49 publications

(47 reference statements)

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“…Compared with other species, the complete AA sequence of PPARα in mullet was highly conserved in the process of evolution, suggesting that PPARα gene might play an important role in some of the physiological activities of animals. The PPARα protein of mullet was predicted to contain 4 domains, including the hypervariable region in Nterminus, DBD, flexible hinge domain and LBD in Cterminus, which further confirmed the previous studies (Green andChambon, 1988, Zhao, Gul, Li, &Wang, 2011). The results suggested that the cloned gene was L. haematocheila PPARα.…”

Section: Discussionsupporting

confidence: 85%

“…Zheng et al (2015) reported that, the expression of PPARα1 (subtype of PPARα) was abundant in the liver of Pelteobagrus fulvidraco larvae, juvenile and adult fish, and was significantly higher than other tissues. Zhao et al (2011) found that, the mRNA expression of PPARα was detected in adipose tissue, gill, heart, liver, spleen, kidney, muscle, intestine, brain and gonad in adult and juvenile of Megalobrama amblycephala. But the expression was various in different growth stage.…”

Section: Discussionmentioning

confidence: 87%

“…PPARα gene was widely expressed in multiple tissues of mammals, birds, osteichthyes and so on, across from embryonic development to adulthood (Desvergne & Wahli, 1999, Ibabe, Bilbao, & Cajaraville, 2005, Raingeard, Cancio, & Cajaraville 2006, Zhao et al, 2011. In mammals, PPARα gene is expressed in a variety of tissues in adult rat and mouse, with high expression in liver, kidney, heart, stomach, brown fat, and the relatively low expression in other tissues (Braissant, Foufelle, Scotto, Dauça, & Wahli, 1996;Kliewer et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

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Yang¹,

Wang²,

Liu³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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The full-length cDNA of proliferator-activated receptor alpha (PPARα) was abtained from the liver of redlip mullet, Liza haematocheila. The PPARα cDNA (GenBank no: KJ848472) was 2409 bp including a 1437 bp open reading frame, which encoded 478 amino acids with four signature domains, i.e., the hypervariable region in N-terminus, DNA-binding domain (DBD), flexible hinge domain and ligand-binding domain (LBD). The mRNA expression level of PPARα was detected in all tissues tested. Highest expression occurred in liver, followed by brain, stomach, skin, spleen and visceral fat, but the expression was weak in heart and muscle. Then, a 60-day feeding trial was conducted to study, the effects of dietary lipid levels (2.0%, 4.8%, 7.5%, 9.8%, 12.0% and 14.6 %) on the mRNA expression of PPARα in mullet. PPARα mRNA expression in liver increased significantly (P<0.05) with the increasing dietary lipid levels. These results indicated that PPARα was tissue-differential expressed gene and played a pivotal role in regulating the lipid metabolism mainly in liver. Results of this study will benefit the further researches on the relationships between PPARα gene and fat metabolism of redlip mullet.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Yang¹,

Wang²,

Liu³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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“…Due to its widely-favored delicacy, nationwide fame, easy-recognized morphology and high economic value, it has been recognized as a main important aquaculture species in the freshwater polyculture system since the 1960s in China (Zhao et al, 2011). However, the population of M. amblycephala is gradually exhibiting lower growth rate.…”

Section: Introductionmentioning

confidence: 99%

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Zeng

Liu

Wang

et al. 2014

Gene

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“…Standard curves were constructed for each gene using serial dilutions of stock cDNA. A set of five housekeeping genes (β-actin, GAPDH, EF1α, 18S rRNA and HPRT) were selected from the literature (Zhao et al, 2011) in order to test their transcription stability. By using geNorm software (Vandesompele et al, 2002), mRNA expression of β-actin and GAPDH proved the most stable across tissue types (M-value of 0.31), while β-actin and 18S rRNA expression were the most stable following insulin treatment (M-value of 0.35).…”

Section: Mrna Level Determination By Quantitative Real-time Pcrmentioning

confidence: 99%

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

Zhuo

Luo

Pan

et al. 2015

Journal of Experimental Biology

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The influence of insulin on hepatic metabolism in fish is not well understood. The present study was therefore conducted to investigate the effects of insulin on lipid metabolism, and the related signaling pathways, in the yellow catfish Pelteobagrus fulvidraco. Hepatic lipid and intracellular triglyceride (TG) content, the activity and expression levels of several enzymes and the mRNA expression of transcription factors (PPARα and PPARγ) involved in lipid metabolism were determined. Troglitazone, GW6471, fenofibrate and wortmannin were used to explore the signaling pathways by which insulin influences lipid metabolism. Insulin tended to increase hepatic lipid accumulation, the activity of lipogenic enzymes (6PGD, G6PD, ME, ICDH and FAS) and mRNA levels of FAS, G6PD, 6PGD, CPT IA and PPARγ, but down-regulated PPARα mRNA level. The insulin-induced effect could be stimulated by the specific PPARγ activator troglitazone or reversed by the PI3 kinase/Akt inhibitor wortmannin, demonstrating that signaling pathways of PPARγ and PI3 kinase/Akt were involved in the insulin-induced alteration of lipid metabolism. The specific PPARα pathway activator fenofibrate reduced insulininduced TG accumulation, down-regulated the mRNA levels of FAS, G6PD and 6PGD, and up-regulated mRNA levels of CPT IA, PPARα and PPARγ. The specific PPARα pathway inhibitor GW6471 reduced insulin-induced changes in the expression of all the tested genes, indicating that PPARα mediated the insulin-induced changes of lipid metabolism. The present results contribute new knowledge on the regulatory role of insulin in hepatic metabolism in fish.

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Cloning, identification and accurate normalization expression analysis of PPARα gene by GeNorm in Megalobrama amblycephala

Cited by 62 publications

References 49 publications

Untitled

Untitled

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Effect and the related signaling pathways of insulin influencing lipid metabolism in yellow catfishPelteobagrus fulvidraco

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