Cloning, expression, and characterization of coenzyme-B12-dependent diol dehydratase from Lactobacillus diolivorans

Wei, X.-X.; Meng, Xianghai; Chen, Yunlai; Wei, Yutuo; Du, Liqin; Huang, Ribo

doi:10.1007/s10529-013-1346-8

Cited by 9 publications

(4 citation statements)

References 23 publications

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“…If a radical side reaction takes place, coenzyme B 12 is not regenerated, and instead, a catalytically inactive cobalamin species is formed, which binds tightly to the enzyme and thereby inactivates it (Toraya, 2003). Radical side reaction can be induced by both glycerol and oxygen, making the enzyme sensitive to oxygen (Wei et al, 2014). The inactive hydratase can be re-activated by the action of glycerol/diol dehydratase reactivase in the presence of ATP and intact coenzyme B 12 (Mori and Toraya, 1999).…”

Section: The Suite Of Enzymes Used In Heterologous 3-hp Synthesis Patmentioning

confidence: 99%

Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria

Jers

Kalantari

Garg

et al. 2019

Front. Bioeng. Biotechnol.

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3-hydroxypropanoic acid (3-HP) is a valuable platform chemical with a high demand in the global market. 3-HP can be produced from various renewable resources. It is used as a precursor in industrial production of a number of chemicals, such as acrylic acid and its many derivatives. In its polymerized form, 3-HP can be used in bioplastic production. Several microbes naturally possess the biosynthetic pathways for production of 3-HP, and a number of these pathways have been introduced in some widely used cell factories, such as Escherichia coli and Saccharomyces cerevisiae . Latest advances in the field of metabolic engineering and synthetic biology have led to more efficient methods for bio-production of 3-HP. These include new approaches for introducing heterologous pathways, precise control of gene expression, rational enzyme engineering, redirecting the carbon flux based on in silico predictions using genome scale metabolic models, as well as optimizing fermentation conditions. Despite the fact that the production of 3-HP has been extensively explored in established industrially relevant cell factories, the current production processes have not yet reached the levels required for industrial exploitation. In this review, we explore the state of the art in 3-HP bio-production, comparing the yields and titers achieved in different microbial cell factories and we discuss possible methodologies that could make the final step toward industrially relevant cell factories.

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Section: The Suite Of Enzymes Used In Heterologous 3-hp Synthesis Patmentioning

confidence: 99%

Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria

Jers

Kalantari

Garg

et al. 2019

Front. Bioeng. Biotechnol.

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“…Glycerol is converted via a one-step reaction towards the formation of 3-HPA in L. diolivorans . This reaction is catalyzed by a glycerol dehydratase, encoded by the gene cluster pduCDE [ 40 ]. As there is no side reaction diverting the flux of glycerol into biomass formation, the glycerol uptake rate is equal to the rate of glycerol conversion to 3-HPA.…”

Section: Resultsmentioning

confidence: 99%

3-Hydroxypropionaldehyde production from crude glycerol by Lactobacillus diolivorans with enhanced glycerol uptake

Lindlbauer

Marx

Sauer

2017

Biotechnol Biofuels

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BackgroundIn their quest for sustainable development and effective management of greenhouse gas emissions, our societies pursue a shift away from fossil-based resources towards renewable resources. With 95% of our current transportation energy being petroleum based, the application of alternative, carbon-neutral products—among them biodiesel—is inevitable. In order to enhance the cost structure of biodiesel biorefineries, the valorization of the crude glycerol waste stream into high-value platform chemicals is of major importance.ResultsThe purpose of this study is the production of 3-hydroxypropionaldehyde (3-HPA) from biodiesel-derived crude glycerol by Lactobacillus diolivorans. Particular focus is given on overcoming potential limitations of glycerol transport into the cell, in order to use the cells’ total glycerol dehydratase capability towards the formation of 3-HPA as the main product. Recombinant overexpression of the endogenous glycerol uptake facilitating protein PduF results in a significant increase of glycerol conversion by a factor of 1.3. Concomitantly, glycerol dehydratase activity increased from initially 1.70 ± 0.03 U/mg protein to 2.23 ± 0.11 U/mg protein. With this approach, an average productivity of 4.8 g3-HPA/(gCDM h) yielding up to 35.9 g/L 3-HPA and 0.91 mol3-HPA/molGlycerol have been obtained.Conclusion Lactobacillus diolivorans proves to be a valuable cell factory for the utilization of crude glycerol delivering high-value C3 chemicals like 3-HPA, 1,3-propanediol (1,3-PDO) and 3-hydroxypropionic acid (3-HP). Enhancing the glycerol influx into the cell by genetic engineering was successful paving the way towards the commercial production of 3-HPA.Electronic supplementary materialThe online version of this article (10.1186/s13068-017-0982-y) contains supplementary material, which is available to authorized users.

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“…There are also other problems related to the first step of the heterologous pathway. Glycerol dehydratase enzyme is sensitive to glycerol accumulation and also to oxygen [79]. Therefore, 3-HP production should be preferably performed in microaerophilic or aerobic conditions.…”

Section: Glycerol Routementioning

confidence: 99%

Heterologous Production of Acrylic Acid: Current Challenges and Perspectives

Rodrigues

2022

SynBio

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Acrylic acid (AA) is a chemical with high market value used in industry to produce diapers, paints, adhesives and coatings, among others. AA available worldwide is chemically produced mostly from petroleum derivatives. Due to its economic relevance, there is presently a need for innovative and sustainable ways to synthesize AA. In the past decade, several semi-biological methods have been developed and consist in the bio-based synthesis of 3-hydroxypropionic acid (3-HP) and its chemical conversion to AA. However, more recently, engineered Escherichia coli was demonstrated to be able to convert glucose or glycerol to AA. Several pathways have been developed that use as precursors glycerol, malonyl-CoA or β-alanine. Some of these pathways produce 3-HP as an intermediate. Nevertheless, the heterologous production of AA is still in its early stages compared, for example, to 3-HP production. So far, only up to 237 mg/L of AA have been produced from glucose using β-alanine as a precursor in fed-batch fermentation. In this review, the advances in the production of AA by engineered microbes, as well as the hurdles hindering high-level production, are discussed. In addition, synthetic biology and metabolic engineering approaches to improving the production of AA in industrial settings are presented.

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Cloning, expression, and characterization of coenzyme-B12-dependent diol dehydratase from Lactobacillus diolivorans

Cited by 9 publications

References 23 publications

Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria

Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria

3-Hydroxypropionaldehyde production from crude glycerol by Lactobacillus diolivorans with enhanced glycerol uptake

Heterologous Production of Acrylic Acid: Current Challenges and Perspectives

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