3-Hydroxypropionaldehyde production from crude glycerol by Lactobacillus diolivorans with enhanced glycerol uptake

Lindlbauer, Katharina Anna; Marx, Hans; Sauer, Michael

doi:10.1186/s13068-017-0982-y

Cited by 25 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lactobacillus reuteri , like many lactobacilli, is known to be unable to grow on glycerol as a sole carbon source, but can use it as an alternative electron acceptor, providing a means to gain energy on a variety of carbon sources [55, 57]. L. reuteri is among the best native producers of large amounts of 3-hydroxypropionaldehyde (reuterin, 3-HPA) from glycerol that are currently known [35]. This is an intermediate in the pathway to 1,3-propanediol (1,3-PDO, also produced by L. reuteri , depending on the conditions used) that is known to be toxic and produced in a microcompartment [5].…”

Section: Resultsmentioning

confidence: 99%

A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory

et al. 2019

View full text Add to dashboard Cite

BackgroundLactobacillus reuteri is a heterofermentative Lactic Acid Bacterium (LAB) that is commonly used for food fermentations and probiotic purposes. Due to its robust properties, it is also increasingly considered for use as a cell factory. It produces several industrially important compounds such as 1,3-propanediol and reuterin natively, but for cell factory purposes, developing improved strategies for engineering and fermentation optimization is crucial. Genome-scale metabolic models can be highly beneficial in guiding rational metabolic engineering. Reconstructing a reliable and a quantitatively accurate metabolic model requires extensive manual curation and incorporation of experimental data.ResultsA genome-scale metabolic model of L. reuteri JCM 1112T was reconstructed and the resulting model, Lreuteri_530, was validated and tested with experimental data. Several knowledge gaps in the metabolism were identified and resolved during this process, including presence/absence of glycolytic genes. Flux distribution between the two glycolytic pathways, the phosphoketolase and Embden–Meyerhof–Parnas pathways, varies considerably between LAB species and strains. As these pathways result in different energy yields, it is important to include strain-specific utilization of these pathways in the model. We determined experimentally that the Embden–Meyerhof–Parnas pathway carried at most 7% of the total glycolytic flux. Predicted growth rates from Lreuteri_530 were in good agreement with experimentally determined values. To further validate the prediction accuracy of Lreuteri_530, the predicted effects of glycerol addition and adhE gene knock-out, which results in impaired ethanol production, were compared to in vivo data. Examination of both growth rates and uptake- and secretion rates of the main metabolites in central metabolism demonstrated that the model was able to accurately predict the experimentally observed effects. Lastly, the potential of L. reuteri as a cell factory was investigated, resulting in a number of general metabolic engineering strategies.ConclusionWe have constructed a manually curated genome-scale metabolic model of L. reuteri JCM 1112T that has been experimentally parameterized and validated and can accurately predict metabolic behavior of this important platform cell factory.

show abstract

Section: Resultsmentioning

confidence: 99%

A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Of 67 lactic acid bacteria isolates tested, 22 isolates belonging to Lactobacillus diolivorans and Lactobacillus collinoides were positive for 3-HP production from glycerol (Garai-Ibabe et al, 2008). Additionally, L. diolivorans is capable of using biodiesel-derived glycerol for the production of 3-HPA (Lindlbauer et al, 2017).…”

Section: Production Hostsmentioning

confidence: 99%

Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria

Jers

Kalantari

Garg

et al. 2019

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

3-hydroxypropanoic acid (3-HP) is a valuable platform chemical with a high demand in the global market. 3-HP can be produced from various renewable resources. It is used as a precursor in industrial production of a number of chemicals, such as acrylic acid and its many derivatives. In its polymerized form, 3-HP can be used in bioplastic production. Several microbes naturally possess the biosynthetic pathways for production of 3-HP, and a number of these pathways have been introduced in some widely used cell factories, such as Escherichia coli and Saccharomyces cerevisiae . Latest advances in the field of metabolic engineering and synthetic biology have led to more efficient methods for bio-production of 3-HP. These include new approaches for introducing heterologous pathways, precise control of gene expression, rational enzyme engineering, redirecting the carbon flux based on in silico predictions using genome scale metabolic models, as well as optimizing fermentation conditions. Despite the fact that the production of 3-HP has been extensively explored in established industrially relevant cell factories, the current production processes have not yet reached the levels required for industrial exploitation. In this review, we explore the state of the art in 3-HP bio-production, comparing the yields and titers achieved in different microbial cell factories and we discuss possible methodologies that could make the final step toward industrially relevant cell factories.

show abstract

“…In considering 3-HP bioproduction, L. reuteri is naturally capable of converting glycerol into 3-HP and 1,3-PDO in equimolar proportions, as it possesses all the enzymes of the metabolic pathway for 3-HP biosynthesis encoded by the pdu operon (9). It has the ability to synthesize vitamin B12 that is an essential co-factor for the first enzyme of the glycerol metabolism (10), but cannot use glycerol as a carbon source (11), which allows glycerol to be completely valorized into 3-HP and 1,3-PDO through the bioconversion pathway.…”

Section: Introductionmentioning

confidence: 99%

Culture conditions affect Lactobacillus reuteri DSM 17938 ability to perform glycerol bioconversion into 3-hydroxypropionic acid

Nguyen

Saulou‐Bérion

Delettre

et al. 2021

Journal of Bioscience and Bioengineering

View full text Add to dashboard Cite

The platform molecule 3-hydroxypropionic acid (3-HP) can be produced using Lactobacillus reuteri through a two-step bioprocess that involves a growth phase followed by a bioconversion phase. The bioproduction is performed by resting cells that convert glycerol into 3-HP and 1,3propanediol in fed-batch mode. This work aimed at studying the effect of the growth conditions of L. reuteri DSM 17938 during the first step, on the glycerol bioconversion into 3-HP during the second step. A Plackett and Burman design was carried out to test, in controlled bioreactors, the effect of 11 growth conditions simultaneously, at fixed bioconversion conditions. The supplementation of the growth medium with vitamin B12 and cysteine displayed a negative effect on the 3-HP bioproduction.

show abstract

3-Hydroxypropionaldehyde production from crude glycerol by Lactobacillus diolivorans with enhanced glycerol uptake

Cited by 25 publications

References 49 publications

A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory

A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory

Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria

Culture conditions affect Lactobacillus reuteri DSM 17938 ability to perform glycerol bioconversion into 3-hydroxypropionic acid

Contact Info

Product

Resources

About