Cloning, expression, and characteristic analysis of the novel β<i>‐</i>galactosidase from silkworm, <scp><i>Bombyx mori</i></scp>

Yao, Yi; Li, Jialei; Zong, Zhipeng; Liu, Xingjian; Song, Haozhi; Wang, Haining; Zhang, Zhifang; Zhang, Huan; Li, Yinü

doi:10.1002/dvg.23446

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…It was universally expressed in various organisms and plays an important role in biological metabolism [45,46]. β-Galactosidase was expressed at multiple tissues including the midgut of the silkworm during the pupal stage [47]. The content of β-Galactosidase (XP_037869036.1) was only identified in day 7 after pupation, suggesting its involvement in carbohydrate metabolism during the transformation from a pupa to an adult.…”

Section: Proteins Involved In Metabolismmentioning

confidence: 99%

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

Wang,

Yang,

Tian

et al. 2023

Insects

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The silkworm Bombyx mori, a lepidopteran insect, possesses an 8–10-day pupal stage, during which significant changes occur in the midgut, where it first condenses into the yellow body, and then undergoes decomposition. To gain insights into this transformation process, proteomics was performed on Bombyx mori midgut contents on day 2 and day 7 after pupation. The results revealed the identification of 771 proteins with more than one unique peptide. An analysis using AgriGO demonstrated that these proteins were predominantly associated with catalytic activity. Among the identified proteins, a considerable number were found to be involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleic acid degradation, and energy support. Additionally, variations in the levels of certain proteases were observed between the midgut contents on day 2 and day 7 after pupation. An in-depth analysis of the two-dimensional electrophoresis of the midgut contents on day 7 after pupation led to the identification of twelve protein spots with potential gelatinolytic activity. Among these, six proteases were identified through mass spectrometry, including the p37k protease, vitellin-degrading protease, chymotrypsin-2, etc. These proteases may be responsible for the digestion of the yellow body during the later stages of pupal development.

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Section: Proteins Involved In Metabolismmentioning

confidence: 99%

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

Wang,

Yang,

Tian

et al. 2023

Insects

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Toxic effects of triphenyltin on the silkworm Bombyx mori as a lepidopterous insect model

Chen

Zhang

et al. 2022

Ecotoxicology and Environmental Safety

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Sublethal and intergenerational effects of fipronil on Binodoxys communis larvae based on transcriptome sequencing

Zhao²,

Zhu³

et al. 2022

Front. Environ. Sci.

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Fipronil is widely used in the agricultural world as an efficient phenylpyrazole insecticide to control pests. Binodoxys communis is a key parasitic natural enemy of major homopteran pests and can successfully control the population of pests such as cotton aphids. It has not yet been studied what effects would sublethal doses of fipronil have on Binodoxys communis larvae. Here, this study evaluated the effect of fipronil on Binodoxys communis larvae and analyze the transcriptome results. The results showed that LC10 (1.19 mg/L) and LC25 (1.73 mg/L) had significant negative effects on the survival rate and parasitism rate of F0 generation. Moreover, exposure to high concentrations (LC25) of fipronil still had obvious passive effect on the F1 generation of Binodoxys communis. These results indicated that sublethal doses of fipronil have malignant effects on the biological functions of parasitoids and their offspring. The results of transcriptome analysis showed that differentially expressed genes (DEGs) of Binodoxys communis after LC10 treatment are mainly related to immunity and detoxification. LC25 treatment instead resulted in changes in the expression of genes related to nutrition, energy and metabolism reactions. Seven of the identified DEGs were selected for real-time fluorescence quantitative PCR analysis. To the best of our knowledge, this is the first report to evaluate the sublethal, intergenerational, and transcriptomic side effects of fipronil on larvae of parasitic natural pest enemies. Our findings provide data to accurately assess the risk of fipronil usage on Binodoxys communis larvae, and provide important theoretical support for the comprehensive prevention and control of natural enemies and pesticides.

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Cloning, expression, and characteristic analysis of the novel β‐galactosidase from silkworm, Bombyx mori

Cited by 3 publications

References 35 publications

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

Toxic effects of triphenyltin on the silkworm Bombyx mori as a lepidopterous insect model

Sublethal and intergenerational effects of fipronil on Binodoxys communis larvae based on transcriptome sequencing

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