Toxic effects of triphenyltin on the silkworm Bombyx mori as a lepidopterous insect model

Chen, Xuedong; Zhang, Xing; Ye, Aihong; Wu, Xinke; Cao, Jinru; Zhou, Wenlin

doi:10.1016/j.ecoenv.2022.114245

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…S2 ). These changes may be related to the abnormality of carbohydrate, lipid, and amino acid metabolism in the midgut of silkworms caused by TPT exposure 18 . In our study, we used the silkworm model to explore the effects of TPT exposure on gut microbial diversity and observed that adverse effects were similar to other aquatic animals 34 .…”

Section: Discussionmentioning

confidence: 99%

“…Bombyx mori (Jingsong × Haoyue strain) were fed formula feed 18 supplemented with 0 and 2 μg/kg TPT for the entire 5th instar larval stage. In briefly, all larvae were reared under a 12-h light/dark cycle at a constant temperature of 26 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Bombyx mori has relatively weak resistance to stress and disease and is thus a promising biological model system for drug screening and antibiotic resistance and environmental pollutant toxicity studies 15 – 17 . In our previous study, we evaluated TPT toxicity using this model and reported that TPT exposure hindered ontogeny by affecting carbohydrate, lipid, and amino acid metabolism in the midgut 18 . Similarly, TPT toxic effects have been comprehensively investigated in multiple species, however, its effects on insect gut microbiota diversity remain obscure.…”

Section: Introductionmentioning

confidence: 99%

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Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

Zhou,

Zhang,

Chen

et al. 2023

Sci Rep

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Triphenyltin (TPT) is a widespread synthetic chemical used in many fields and its potential risk to organisms has been comprehensively investigated using different animal models and species. Currently, little is known about the effects of TPT exposure on microbial midgut diversity, therefore we explored these effects in the lepidopterous silkworm model using 16S rDNA sequencing. In total, 5273 and 5065 operational taxonomic units (OTUs) were identified in control and TPT-exposure group samples, ranging from 424 to 728 OTUs/sample. Alpha-diversity analyses revealed that TPT exposure induced the fluctuations of gut microbial diversity and abundance while beta-diversity analyses identified a distinct impact on major gut microbiota components. In our microbiome analyses, 23 phyla and 353 genera were recognized in the control group, while 20 phyla and 358 genera were recognized in the TPT exposure group. At the genus level, midgut microbiota were composed of several predominant bacterial genera, including Muribaculaceae, Lactobacillus, and UCG-010. In the TPT exposure group, o__Bacillales, f__Bacillaceae, and f__Caldicoprobacteraceae abundance was relatively high, while f__Oscillospiraceae, f__Fusobacteriaceae, and f__SC_I_84 abundance was relatively high in the control group. Gene function analyses in silkworm microbiota after TPT exposure showed that biosynthesis of ansamycins, fructose and mannose metabolism, glycerolipid metabolism, type II diabetes mellitus, glycolysis/gluconeogenesis, lipid metabolism, translation proteins, atrazine degradation, DNA repair and recombination proteins, nicotinate and nicotinamide metabolism were significantly increased. Collectively, our silkworm model identified gut microbial diversity risks and the adverse effects from TPT exposure, which were similar to other aquatic animals. Therefore, TPT levels in environmental samples must be monitored to prevent ecological harm.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

Zhou,

Zhang,

Chen

et al. 2023

Sci Rep

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“…18 Therefore, many studies regarding assessment of human health are based on the silkworm model. 19 The silkworm model has been used to evaluate the effects of environmental pollutants, 20,21 heavy metals, 22,23 toxic chemicals, 24,25 pathogen infections 26,27 and new medicines. 28,29 Cold storage increases reactive oxygen species (ROS) in different fruit and vegetables.…”

Section: Introductionmentioning

confidence: 99%

Cold‐stored mulberry leaves affect antioxidant system and silk proteins of silkworm (Bombyx mori) larva

2023

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BACKGROUNDCold storage has been widely used to maintain the quality of vegetables, but whether eating cold‐stored vegetables affects health remains unknown.RESULTSThis study used silkworms as an animal model to evaluate the effects of nutrient changes in cold‐stored mulberry leaves (CSML) on health. Compared with fresh mulberry leaves (FML), CSML contained lower vitamin C, soluble sugars and proteins, and higher H2O2, suggesting decreased antioxidant ability and nutrition. The CSML did not obviously affect larval survival rate, body weight or dry matter rate, cocoon shape, weight and size, or final rates of cluster and cocooning relative to the FML, suggesting CSML did not alter overall growth and development. However, the CSML increased the initial rates of cluster and cocooning and upregulated BmRpd3, suggesting CSML shortened larval lifespan and enhanced senescence. CSML upregulated BmNOX4, downregulated BmCAT, BmSOD and BmGSH‐Px and increased H2O2 in silkworms, suggesting CSML caused oxidative stress. CSML upregulated ecdysone biosynthesis and inactivation genes and elevated ecdysone concentration in silkworms, suggesting that CSML affected hormone homeostasis. CSML upregulated apoptosis‐related genes, downregulated sericin and silk fibroin genes and decreased sericin content rate in silkworms, suggesting oxidative stress and protein deficiency.CONCLUSIONCold storage reduced nutrition and antioxidant capability of mulberry leaves. CSML did not influence growth and development of silkworm larva, but affected health by causing oxidative stress and reducing protein synthesis. The findings show that the ingredient changes in CSML had negative effects on health of silkworms. © 2023 Society of Chemical Industry.

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Single and combined effects of ammonia and nitrite on Litopenaeus vannamei: Histological, physiological and molecular responses

Lin,

Li,

Liu

et al. 2024

Aquaculture Reports

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Toxic effects of triphenyltin on the silkworm Bombyx mori as a lepidopterous insect model

Cited by 6 publications

References 47 publications

Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

Cold‐stored mulberry leaves affect antioxidant system and silk proteins of silkworm (Bombyx mori) larva

Single and combined effects of ammonia and nitrite on Litopenaeus vannamei: Histological, physiological and molecular responses

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