Cloning expression and analysis of phytochelatin synthase (pcs) gene from Anabaena sp. PCC 7120 offering multiple stress tolerance in Escherichia coli

Genome-wide inventory of metal homeostasis-related gene products including a functional phytochelatin synthase in the hypogeous mycorrhizal fungus Tuber melanosporum

“…This includes one functionally characterized member in the unicellular ascomycete Schizosaccharomyces pombe (Clemens et al, 1999;Ha et al, 1999) and other Schizosaccharomycetales as well (R.R., unpublished observations), two putative PCSs in the basidiomycete Puccinia graminis and three predicted isoforms in the mucoromycete Rhizopus oryzae (Clemens, 2006;R.R., unpublished), but none in filamentous ascomycetes, except T. melanosporum. At variance with PCS-like enzymes recently described in various cyanobacteria and in protozoa (Amaro et al, 2009;Chaurasia et al, 2008;Tsuji et al, 2004), which have a restricted similarity to the plant enzyme and are devoid of PC biosynthetic activity, TmelPCS (superfamily domain pfam05023) has all the features of a genuine PCS enzyme (Rea et al, 2004;Romanyuk et al, 2006;Ruotolo et al, 2004). These include the catalytic triad signature Cys60, His166 and Asp185, and a predicted two-domain organization, with a conserved N-terminal domain and a more variable C-terminal domain (data not shown, but see Section 3.4).…”

Review of arsenic speciation, toxicity and metabolism in microalgae

“…PCC7120 under As stress. However, the role of other genes like phytochelatin synthase (pcs) gene of microalgae in As toxicity management remained unanswered (Chaurasia et al 2008;Pandey et al 2012). …”

Over-Expression of Arabidopsis Phytochelatin Synthase (Atpcs1) Gene in Escherichia Coli Confers Enhanced Tolerance to Cadmium

“…The expression of AtPCS1 enhanced cellular Cd content ranged between 23% and 35% in recombinant E.coli cells. Earlier, it was reported that growing bacteria and yeast expressing At PCS on from free radicals, reactive oxygen species to heavy metals (Rabenstein, 1989) and detoxifying them, resulting into tolerance (Chaurasia et al, 2008;Gill and Tuteja, 2010).…”