Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti

Zhao, Guochang; Liu, Jiannan; Hu, Xiaohua; Batool, Khadija; Jin, Liang; Wu, Chenxu; Wu, Juan; Hong, Chen; Jiang, Xiaoyan; Yang, Zemin; Huang, Xin; Huang, Enjiong; Yu, Xiao‐Qiang; Guan, Xiong; Zhang, Lingling

doi:10.1186/s13071-019-3560-2

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…Another study suggested that Cry11Aa toxicity in Ae. aegypti also depends on an ATP-binding cassette protein [ 61 ], although more studies are necessary to determine the role of this molecule as a Cry toxin receptor in mosquitoes. The receptors characterized for the Cry4Ba toxin in Ae.…”

Section: Toxins and Mode Of Actionmentioning

confidence: 99%

Bacterial Toxins Active against Mosquitoes: Mode of Action and Resistance

Silva-Filha

Romão

Rezende

et al. 2021

Toxins

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Larvicides based on the bacteria Bacillus thuringiensis svar. israelensis (Bti) and Lysinibacillus sphaericus are effective and environmentally safe compounds for the control of dipteran insects of medical importance. They produce crystals that display specific and potent insecticidal activity against larvae. Bti crystals are composed of multiple protoxins: three from the three-domain Cry type family, which bind to different cell receptors in the midgut, and one cytolytic (Cyt1Aa) protoxin that can insert itself into the cell membrane and act as surrogate receptor of the Cry toxins. Together, those toxins display a complex mode of action that shows a low risk of resistance selection. L. sphaericus crystals contain one major binary toxin that display an outstanding persistence in field conditions, which is superior to Bti. However, the action of the Bin toxin based on its interaction with a single receptor is vulnerable for resistance selection in insects. In this review we present the most recent data on the mode of action and synergism of these toxins, resistance issues, and examples of their use worldwide. Data reported in recent years improved our understanding of the mechanism of action of these toxins, showed that their combined use can enhance their activity and counteract resistance, and reinforced their relevance for mosquito control programs in the future years.

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Section: Toxins and Mode Of Actionmentioning

confidence: 99%

Bacterial Toxins Active against Mosquitoes: Mode of Action and Resistance

Silva-Filha

Romão

Rezende

et al. 2021

Toxins

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“…In A. aegypti , galectin-14 can compete with Cry11Aa by binding to the Cry receptor ALP1 [ 143 ]. Consistently, galectin-6 was found to interact with ALP1, thus affecting the toxicity of Cry proteins [ 144 ].…”

Section: Immunity Induced By Btmentioning

confidence: 96%

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Xiao

Yao

Bai

et al. 2023

Insects

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Bacillus thuringiensis (Bt) is the safest, economically successful entomopathogen to date. It is extensively produced in transgenic crops or used in spray formulations to control Lepidopteran pests. The most serious threat to the sustainable usage of Bt is insect resistance. The resistance mechanisms to Bt toxins depend not only on alterations in insect receptors, but also on the enhancement of insect immune responses. In this work, we review the current knowledge of the immune response and resistance of insects to Bt formulations and Bt proteins, mainly in Lepidopteran pests. We discuss the pattern recognition proteins for recognizing Bt, antimicrobial peptides (AMPs) and their synthetic signaling pathways, the prophenoloxidase system, reactive oxygen species (ROS) generation, nodulation, encapsulation, phagocytosis, and cell-free aggregates, which are involved in immune response reactions or resistance to Bt. This review also analyzes immune priming, which contributes to the evolution of insect resistance to Bt, and puts forward strategies to improve the insecticidal activity of Bt formulations and manage insect resistance, targeting the insect immune responses and resistance.

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“…Respecto a la CL95, las concentraciones evaluadas aquí, fueron menores a las recientemente encontradas por Gómez-Vargas et al (2018), quienes determinaron la efectividad de tabletas de Bti serotipo H14, Bti-CECIF, producido en Colombia, hallando valores entre 0,40 a 0,93ppm.Lo anterior corrobora la viabilidad del uso de Bti en los programas de control vectorial de Colombia, donde se utilizan tres larvicidas: el Bti, el triflumurón (inhibidor de la síntesis de quitina en insectos) y el OP temephos (MinSalud et al 2011), donde la resistencia está ampliamente diseminada(Grisales et al 2013), lo que enfatiza, aún más, el uso del Bti. No obstante, los problemas inherentes al uso del Bti en campo, como tasa de ingestión, edad y densidad de las larvas, temperatura, radiación solar, entre otras, deben ser evaluados(Zhao et al 2019).…”

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Control de Aedes (Stegomyia) aegypti utilizando Bacillus thuringiensis var. israelensis en Armenia, Quindío, Colombia

Aguirre-Obando

Gandica

2020

Rev. U.D.C.A Act. & Div. Cient.

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In Colombia, Aedes aegypti is resistant to most used insecticides. Due to the slow development of resistance to Bacillus thuringiensis var. israelensis (Bti) as well as its high specificity and environmental safety, the use of this larvicide becomes an alternative in the management of this vector. In this work, we evaluated experimentally and describe by a mathematical model the dyamics of control of natural populations of A. aegypti using Bti. The susceptibility profile to Bti was determined through dose-response bioassays with larvae collected in Armenia (Quindío, Colombia). In addition, in order to estimate the susceptibility profile, an analysis was carried out using the mortality data obtained from the four localities analyzed. The mortality data were used to estimate the lethal concentrations (LC50 and 95) from each locality. Using these LC, hypothetical simulations of vector behavior were carried out, obtained from a mathematical model that describes the population dynamics, using successive applications of Bti at different time intervals. The dose-response bioassays indicate tha the analyzed vector populations are susceptible to Bti since they present a biological response similar to the one obtained from A. aegypti Rockefeller strain. Computer simulations using sustained periodic inspection indicate Bti is effective for the vector control. Nevertheless, its long-term efficiency depends on relation between the LC and the frequency of application. It is concluded that the sustained application of Bti represents a longterm viable alternative for the control of A. aegypti populations.

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Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti

Cited by 5 publications

References 34 publications

Bacterial Toxins Active against Mosquitoes: Mode of Action and Resistance

Bacterial Toxins Active against Mosquitoes: Mode of Action and Resistance

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Control de Aedes (Stegomyia) aegypti utilizando Bacillus thuringiensis var. israelensis en Armenia, Quindío, Colombia

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