Cloning, characterization and expression analysis of key genes involved in ABA metabolism in Gladiolus cormels during storage

Luo, Xian; Yi, Jin; Zhong, X.P.; Lian, Qing Long; Khan, Muhammad Ali; Cao, Xinmin; Li, Xiao Xin; Gao, Ming; Wu, Jian; Chen, Jin; Yi, Ming

doi:10.1016/j.scienta.2012.06.016

Cited by 10 publications

(2 citation statements)

References 35 publications

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“…The sprouting percentage was counted on the 20th day after planting on soil. Sprouting was defined as a bud on the top of the cormel elongated >5 mm (Luo et al , 2012). Thirty cormels per stage were used for each sprouting test.…”

Section: Methodsmentioning

confidence: 99%

GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis inGladiolus hybridus

Jin

Liu

et al. 2018

Journal of Experimental Botany

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Section: Methodsmentioning

confidence: 99%

GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis inGladiolus hybridus

Jin

Liu

et al. 2018

Journal of Experimental Botany

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“…"Gladiolus (Gladiolus grandiflorus L.) is a bulbous ornamental plant, which belongs to family Iridiaceae. It is popularly known as Queen of bulbous flower because of attractive spike, having florets of huge forms, dazzling colors and longer keeping quality" [1]. "Gladiolus is one of the most important bulbous flowering crops grown commercially for cut-flower trade in India.…”

Section: Introductionmentioning

confidence: 99%

Effect of Micronutrients on Growth and Flowering of Gladiolus (Gladiolus grandiflorus L.) under Subtropics of Jammu

Harish¹,

Pandey²,

Shah³

et al. 2023

IJECC

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An open field experiment was conducted during 2019-20 at the Experimental Farm, Division of Vegetable Science & Floriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, to evaluate the effect of micronutrients on growth, flowering and yield of gladiolus (Gladiolus grandiflorus L.) cv. ‘Nova Lux’. The experiment was laid out in Randomized Block Design with three replications and nineteen treatments. The different micro-nutrient like Fe, Zn and Mn was taken in different concentrations along with control. The results revealed that maximum plant height (97.74 cm), number of leaves per plant (9.04), leaf length (74.36 cm) and leaf area index (4.14) was recorded with the application of ZnSO4 at 0.2 % and FeSO4 at 0.2 %. Further, among the flowering traits, minimum duration for days taken for 1st floret opening, the maximum spike length (81.86 cm), rachis length (56.76 cm), number of florets per spike 917.69) and weight of the spike (74.78 g) was recorded with the application of ZnSO4 at 0.2 % and FeSO4 at 0.2 %. Thus, foliar application of micronutrients i,e. ZnSO4 at 0.2 % and FeSO4 at 0.2 % proved to be most effective in increasing the growth, flowering and corm production in gladiolus.

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Virus-induced gene silencing for comparative functional studies in Gladiolus hybridus

Zhong

Xue

et al. 2013

Plant Cell Rep

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Functional analysis of genes in gladiolus has previously been impractical due to the lack of an efficient stable genetic transformation method. However, virus-induced gene silencing (VIGS) is effective in some plants which are difficult to transform through other methods. Although the Tobacco rattle virus (TRV)-based VIGS system has been developed and used for verifying gene functions in diverse plants, an appropriate TRV-VIGS approach for gladiolus has not been established yet. In this report we describe the first use of the TRV-VIGS system for gene silencing in gladiolus. Vacuum infiltration of cormels and young plants with the GhPDS-VIGS vector effectively down-regulated the PHYTOENE DESATURASE ortholog GhPDS gene and also resulted in various degrees of photobleaching in Gladiolus hybridus. The reduction in GhPDS expression was tested after TRV-based vector infection using real-time RT-PCR. In addition, the progress of TRV infection was detected by fluorescence visualization using a pTRV2: CP-GFP vector. In conclusion, the TRV-mediated VIGS described here will be an effective gene function analysis mechanism in gladiolus.

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Cloning, characterization and expression analysis of key genes involved in ABA metabolism in Gladiolus cormels during storage

Cited by 10 publications

References 35 publications

GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis inGladiolus hybridus

GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis inGladiolus hybridus

Effect of Micronutrients on Growth and Flowering of Gladiolus (Gladiolus grandiflorus L.) under Subtropics of Jammu

Virus-induced gene silencing for comparative functional studies in Gladiolus hybridus

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