GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis in<i>Gladiolus hybridus</i>

Wu, Jian; Jin, Yujie; Liu, Chen; Vonapartis, Eliana; Liang, Jiahui; Wu, Wenjing; Gazzarrini, Sonia; He, Junna; Yi, Mingfang

doi:10.1093/jxb/ery428

Cited by 28 publications

(39 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As an ABA binding factor, ABF2 often combines with AREB1 to form the AREB1/ABF2 transcription factor, which is the key factor downstream of the ABA signaling pathway [54,55]. In the process of bud dormancy, the expression of ABF2 is theoretically conducive to ABA signal transduction, thus maintaining bud endodormancy [6,56]. In this study, ABF2 showed a unimodal curve, which reached its peak on 9 January (Figure 10), suggesting that its high expression promoted the release of endodormancy in buds.…”

Section: Relationship Between Hormone Metabolism and Endodormancy Releasementioning

confidence: 99%

Chilling Requirement Validation and Physiological and Molecular Responses of the Bud Endodormancy Release in Paeonia lactiflora ‘Meiju’

Zhang

Wang

Shi³

et al. 2021

IJMS

View full text Add to dashboard Cite

The introduction of herbaceous peony (Paeonia lactiflora Pall.) in low-latitude areas is of great significance to expand the landscape application of this world-famous ornamental. With the hazards of climate warming, warm winters occurs frequently, which makes many excellent northern herbaceous peony cultivars unable to meet their chilling requirements (CR) and leads to their poor growth and flowering in southern China. Exploring the endodormancy release mechanism of underground buds is crucial for improving low-CR cultivar screening and breeding. A systematic study was conducted on P. lactiflora ‘Meiju’, a screened cultivar with a typical low-CR trait introduced from northern China, at the morphological, physiological and molecular levels. The CR value of ‘Meiju’ was further verified as 677.5 CUs based on the UT model and morphological observation. As a kind of signal transducer, reactive oxygen species (ROS) released a signal to enter dormancy, which led to corresponding changes in carbohydrate and hormone metabolism in buds, thus promoting underground buds to acquire strong cold resistance and enter endodormancy. The expression of important genes related to ABA metabolism, such as NCED3, PP2C, CBF4 and ABF2, reached peaks at the critical stage of endodormancy release (9 January) and then decreased rapidly; the expression of the GA2ox8 gene related to GA synthesis increased significantly in the early stage of endodormancy release and decreased rapidly after the release of ecodormancy (23 January). Cytological observation showed that the period when the sugar and starch contents decreased and the ABA/GA ratio decreased was when ‘Meiju’ bud endodormancy was released. This study reveals the endodormancy regulation mechanism of ‘Meiju’ buds with the low-CR trait, which lays a theoretical foundation for breeding new herbaceous peony cultivars with the low-CR trait.

show abstract

Section: Relationship Between Hormone Metabolism and Endodormancy Releasementioning

confidence: 99%

Chilling Requirement Validation and Physiological and Molecular Responses of the Bud Endodormancy Release in Paeonia lactiflora ‘Meiju’

Zhang

Wang

Shi³

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…A single transformed YM4271(a) colony containing different truncated or mutated promoters [F1 to F4 and pFUS3 MUT (0.6kb), described above] was used for mating with EGY48(α) containing BPCs. Mating and selection procedures were described in Wu et al (2018). The interaction was judged by the growth of yeast on selection media on the third day.…”

Section: Yeast One-hybrid Screeningmentioning

confidence: 99%

Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth

Mohamed

Dowhanik

et al. 2020

Plant Cell

Self Cite

View full text Add to dashboard Cite

Spatiotemporal regulation of gene expression plays an important role in developmental timing in plants and animals. FUSCA3 regulates the transition between different phases of development by acting as a link between different hormonal pathways in Arabidopsis.However, the mechanisms governing its spatiotemporal expression patterns are poorly understood. Here, we show that FUS3 is expressed in the chalaza and funiculus of the mature ovule and seed, but is repressed in the embryo sac, integuments and endosperm. FUS3 repression requires class I BASIC PENTACYSTEINE (BPC) proteins, which directly bind to the FUS3 locus and restrict its expression pattern. During vegetative and reproductive development, derepression of FUS3 in bpc1/2 or pML1:FUS3 misexpression lines results in dwarf plants carrying defective flowers and aborted ovules. Post-fertilization, ectopic FUS3 expression in the endosperm increases endosperm nuclei proliferation and seed size and delays or arrests embryo development. These phenotypes are rescued in bpc1/2 fus3-3. Lastly, class I BPCs interact with FIS-PRC2 (FERTILIZATION-INDEPENDENT SEED-Polycomb Repressive Complex 2), which represses FUS3 in the endosperm. We propose that BPC1/2 promotes the transition from reproductive to seed development by repressing FUS3 in ovule integuments. After fertilization, BPC1/2 and FIS-PRC2 repress FUS3 in the endosperm to coordinate endosperm and embryo growth.

show abstract

“…Previous studies have shown that the NAC family is involved in regulating multiple hormone signaling pathways. GhNAC83 affects the dormancy of gladiolus bulbs by regulating ABA signal transduction and CTK biosynthesis [42]. The function of MADs-Box (DAM) is widely studied in perennials such as apples and pears.…”

Section: Tfs Involved In Dormancy Releasementioning

confidence: 99%

WITHDRAWN: Transcriptome and targeted metabolome reveal the regulation network of Lilium davidii var. unicolor during dormancy release

Fan

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background：Regulation and control of bulb dormancy are critical for ensuring annual production and high-level cultivation of lily. Application of low temperatures is the most effective method for breaking lily bulb dormancy, but the molecular regulatory mechanism underlying this response is unclear. Results：Herein, targeted metabolome and transcriptome analyses were performed with buds of Lilium davidii var. unicolor bulbs stored for 0, 50 and 100 days at 4°C. Dormancy release mainly depended on the accumulation of GA4 and GA7, which are synthesized by the "non13-hydroxylation" pathway, rather than GA3, and ABA was degraded in the process. The contents of nonbioactive GA9, GA15 and GA24, the precursors of GA4 synthesis, increased with bulb dormancy release. Altogether, 113,252 unique transcripts were de novo assembled through high-throughput transcriptome sequences, and 639 genes were continuously differentially expressed. Enrichment analysis showed that the carbohydrate metabolism and hormone metabolism pathways play major roles in dormancy release. Energy sources during carbohydrate metabolism mainly depend on glycolysis and the PPP pathway rather than on the TCA cycle. Association analysis of the transcriptome and target metabolome showed that genes related to ABA, GA, starch and sucrose metabolism and some TF families, such as MYB, WRKY, NAC and TCP, involved in dormancy release were highly correlated with the target metabolome. Coexpression analysis further confirmed that ABI5, PYL8, PYL4 and PP2C, vital ABA signaling elements, regulated GA3ox and GA20ox in the GA4 biosynthesis pathway. The TFs WRKY32, WRKY71, MYB, DAM14, NAC8, ICE1, bHLH93 and TCP15 also participated in the ABA/GA4 regulatory network, and ICE1 may be the key factor linking temperature signals and hormone metabolism. Conclusions：These results will help to reveal the bulb dormancy molecular mechanism and to develop new strategies for high-quality bulb production.

show abstract

GhNAC83 inhibits corm dormancy release by regulating ABA signaling and cytokinin biosynthesis inGladiolus hybridus

Abstract: GhNAC83 inhibits corm dormancy release in Gladiolus hybridus by promoting ABA signaling and inhibiting CK biosynthesis.

Cited by 28 publications

References 94 publications

Chilling Requirement Validation and Physiological and Molecular Responses of the Bud Endodormancy Release in Paeonia lactiflora ‘Meiju’

Chilling Requirement Validation and Physiological and Molecular Responses of the Bud Endodormancy Release in Paeonia lactiflora ‘Meiju’

Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth

WITHDRAWN: Transcriptome and targeted metabolome reveal the regulation network of Lilium davidii var. unicolor during dormancy release

Contact Info

Product

Resources

About