Cloning, characterisation, docking and expression analysis of 3-beta-hydroxysteroid dehydrogenase during ontogenetic development and annual reproductive cycles in catfish, Clarias batrachus

Bhat, Irfan Ahmad; Rather, Mohd Ashraf; Nazir, Mir Ishfaq; Gireesh‐Babu, P.; Goswami, Mukunda; Sundaray, Jitendra Kumar; Sharma, Rupam

doi:10.1016/j.theriogenology.2017.09.017

Cited by 12 publications

(4 citation statements)

References 47 publications

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“…Indeed, P450 aromatase levels rise during maturation, and the expression of this enzyme is a main limiting factor for the synthesis of E2 in the eel ovary, because the 17β-HSD-I gene is highly transcribed at the vitellogenic stage [ 61 ]. Furthermore, decreased 3β-HSD affects the conversion of pregnenolone, 17 a -hydroxypregnenolone, and dehydroepiandrosterone to progesterone, 17 a -hydroxyprogesterone, and androstenedione, respectively, which are crucial steps in the biosynthesis of sex steroids [ 62 ]. Therefore, by considering the physiological role of the enzymes and their involvement in oogenesis, our results suggest that cocaine may affect this process and, in turn, reproductive success in eels, even if more detailed studies are required to confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Aquatic Pollution and Risks to Biodiversity: The Example of Cocaine Effects on the Ovaries of Anguilla anguilla

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Rosati

Lorenzo

et al. 2022

Animals

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Pollution is one of the main causes of the loss of biodiversity, currently one of the most important environmental problems. Important sources of aquatic pollution are illicit drugs, whose presence in waters is closely related to human consumption; their psychoactive properties and biological activity suggest potential adverse effects on non-target organisms, such as aquatic biota. In this study, we evaluated the effect of an environmentally relevant concentration of cocaine (20 ng L−1), an illicit drug widely found in surface waters, on the ovaries of Anguilla anguilla, a species critically endangered and able to accumulate cocaine in its tissues following chronic exposure. The following parameters were evaluated: (1) the morphology of the ovaries; (2) the presence and distribution of enzymes involved in oogenesis; (3) serum cortisol, FSH, and LH levels. The eels exposed to cocaine showed a smaller follicular area and a higher percentage of connective tissue than controls (p < 0.05), as well as many previtellogenic oocytes compared with controls having numerous fully vitellogenic and early vitellogenic oocytes. In addition, the presence and location of 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, and P450 aromatase differed in the two groups. Finally, cocaine exposure decreased FSH and LH levels, while it increased cortisol levels. These findings show that even a low environmental concentration of cocaine affects the ovarian morphology and activity of A. anguilla, suggesting a potential impact on reproduction in this species.

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Section: Discussionmentioning

confidence: 99%

Aquatic Pollution and Risks to Biodiversity: The Example of Cocaine Effects on the Ovaries of Anguilla anguilla

Fontes

Rosati

Lorenzo

et al. 2022

Animals

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“…Two isoforms of this transcript have been found (Type I and II) and tissue distribution studies revealed that type I mainly express in mature testes and type II in vitellogenic and mature ovarian follicles (Senthilkumaran et al 2009). In Asian and African catfish, hsd3b expresses in gonads, brain, liver and kidneys (Raghuveer & Senthilkumaran 2012;Bhat et al 2017c) and helps in steroid hormone production, metabolism of steroids and neurosteroid production, respectively (Simard et al 2005;Senthilkumaran et al 2009).…”

Section: Starmentioning

confidence: 99%

“…In Asian and African catfish, hsd3b expresses in gonads, brain, liver and kidneys (Raghuveer & Senthilkumaran 2012; Bhat et al . 2017c) and helps in steroid hormone production, metabolism of steroids and neurosteroid production, respectively (Simard et al . 2005; Senthilkumaran et al .…”

Section: Genes Involved In Steroidogenesis Pathway and Their Modulation In Fishmentioning

confidence: 99%

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Bhat

Dar

Ahmad

et al. 2021

Reviews in Aquaculture

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In fish, as in higher animals, the normal ontogenesis of the gonads is the basis for successful reproduction. Gonadal differentiation and development depend on the genetic cascade that programmes the events. Eventually, gonads become the sites for the maturation of gametes, which are originally derived from the primordial germ cells (PGCs) and form the cellular basis of reproduction. In fish hormones of the hypothalamic-pituitary-gonadal (HPG) axis regulate reproduction and fertility by promoting or inhibiting the production of gonadal sex steroids and their function. Gonadal sex steroids not only guide proper gonadal development and function but also play a key role in the activation and regulation of the HPG axis through two feedback control systems. In this review, we discuss the general outline and expression pattern of the major genes involved in testicular development and gonadal steroidogenesis in fish. The review attempts, for the first time, to collect and discuss recent information regarding the regulation of sex differentiation and steroidogenic genes during the stages of testicular development and in response to different external factors. Furthermore, clear insights into the important genes and their regulation involved in the HPG axis and spermatogenesis are presented briefly. This review can serve as a guide for fish breeders on the substances that have a positive impact on reproduction in males.

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“…Transcripts that were downregulated, in addition to igfbp-5, were 3β-hsd and inhbb which are associated with sex steroid biosynthesis (Maugars and Schmitz 2008) and early development (Garcia de la Serrana and Macqueen 2018). A study of the walking catfish Clarias batrachus revealed that 3β-hsd plays an important role in gonadal development (Bhat et al 2018) and was reduced in male rainbow trout exposed to 17α-ethinyl-estradiol (Baron et al 2005a). It should be noted that a decrease in inhbb, which is produced in the sertoli cells of males (Pierik et al 1998) was observed.…”

Section: Discussionmentioning

confidence: 98%

Application of Molecular Pathology Techniques to Understand Mechanisms of Disease in Smallmouth Bass

Walsh¹

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In the Chesapeake Bay drainage, smallmouth bass Micropterus dolomieu are used as an indicator species of estrogenic contaminant exposure and have been implicated in fish kills and disease since 2005. In the Potomac River drainage, adult smallmouth bass have experienced mortality and disease and males have a high prevalence of intersex (testicular oocytes). Conversely, in the Susquehanna River drainage mortalities and disease of young-of-the-year smallmouth bass (YOY SMB) have occurred and resulted in a population shift to older and larger fish. The exact cause of these events remains unknown; however, factors such as poor water quality, contaminants, pathogens and parasites, increased temperatures, and nutrification have been assessed. In order to address this issue, the USGS Fish Health Branch, Leetown Science Center, and West Virginia Cooperative Fish and Wildlife Research Unit have ongoing assessment and monitoring projects throughout the Chesapeake Bay watershed. The use of routine histopathology has provided guidance for further research with molecular endpoints that may help explain the mechanisms involved in disease in smallmouth bass. The purpose of this dissertation research was to: 1.) Identify the prevalence of coinfections and risk factors of disease in YOY SMB; 2.) Use in situ hybridization to identify coinfections of bacteria with the myxozoan parasite Myxobolus inornatus in YOY smallmouth bass; 3.) Model liver helminths and coinfections of YOY SMB with land use at two spatial scales; 4.) Develop laser capture microdissection methods for nucleic acid extractions which could be used with smallmouth bass tissues; and 5.) Utilize Next-Generation Sequencing to develop a partial testes transcriptome to identify molecular markers that may help explain intersex development in male smallmouth bass. would like to thank the many people who have helped in the laboratory and in the field including Kathy Spring,

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Cloning, characterisation, docking and expression analysis of 3-beta-hydroxysteroid dehydrogenase during ontogenetic development and annual reproductive cycles in catfish, Clarias batrachus

Cited by 12 publications

References 47 publications

Aquatic Pollution and Risks to Biodiversity: The Example of Cocaine Effects on the Ovaries of Anguilla anguilla

Aquatic Pollution and Risks to Biodiversity: The Example of Cocaine Effects on the Ovaries of Anguilla anguilla

Testicular development and spermatogenesis in fish: insights into molecular aspects and regulation of gene expression by different exogenous factors

Application of Molecular Pathology Techniques to Understand Mechanisms of Disease in Smallmouth Bass

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