Cloning and structural organization of a xylanase-encoding gene from Penicillium chrysogenum

xylP Promoter-Based Expression System and Its Use for Antisense Downregulation of the Penicillium chrysogenum Nitrogen Regulator NRE

“…Initially, only 510 bp of the 5Ј noncoding region of xylP was analyzed (18). In order to characterize the complete 1,676-bp promoter fragment employed for overexpression of nreB (21), the region between an SalI site and the xylP translation start codon was sequenced in its entirety and was analyzed to determine the presence of putative transcription factor binding sites.…”

“…cesses like paper pulp bleaching or bioconversion of biomass into fuels and chemicals (31 (12,18,25,28,35,45,48,53,54).…”

“…It has already been shown that xylP is not expressed during growth on glucose but is highly induced by xylan as a sole carbon source (18). To investigate induction of the xylP promoter in P. chrysogenum in more detail, QXU-P1 was grown in shake flasks on various carbon sources and GUS activity was measured after different time periods (Fig.…”

“…We have cloned and characterized a gene encoding a group F 1,4-␤-endoxylanase (XYLP) which is expressed at a high level during growth on xylan, yielding up to 25% of the total proteins secreted into the culture medium, and is repressed by glucose (17,18). Recently, we have employed the xylP promoter region successfully for overexpression of the nitrogen regulatory GATA factor NREB in Penicillium, but a detailed analysis of this promoter, which would permit general application, has not been performed so far (21).…”

Xylanases of Microorganisms

“… îñòàíí³ äåê³ëüêà ðîê³â çíà÷íèé ïðîгðåñ áóâ äîñÿгíóòèé â êëîíóâàíí³ ðÿäó êñèëàíîë³òè÷íèõ гåí³â ó òàêèõ гðèá³â ÿê Aspergillus swamori [29], A. nidulans [31,43], A. oryzae [32], A. niger [33], Chaetomium gracile [60], Penicillium chrysogenum [27], Trichoderma viride [56]. Êð³ì êñèëàíîë³òè÷íèõ гåí³â, ç àñïåðг³ëë³â òà T. reesi áóëî ³çîëüîâàíî áàгàòî гåí³â, ÿê³ êîäóþòü äîïîì³жí³ фåðìåíòè, òàê³ ÿê àöåòèëêñèëàí åñòåðàçà [24], [37], α L àðàá³íîфóðàíîç³äàçà [21], àðàá³íîêñèëàí àðàá³íîфóðàíîçèäàçà [22], α гëþêóðîí³äàçà [58], фåðóî³ë åñòåðàçà [57].…”