Cloning and sequencing the endocarditis immunodominant antigen of Streptococcus sobrinus strain MUCOB 263

Brooks, Wanda; Burnie, J P

doi:10.1099/00222615-40-5-330

Cited by 15 publications

(12 citation statements)

References 2 publications

(2 reference statements)

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“…Antibodies to surface PA were elevated greatly in patients with S. mutans infective endocarditis (24). Antibodies against similar surface proteins were reported in S. sobrinus (25), and S. oralis (26). In S. mutans , including the untypable strains used here, PA gives a common immunological specificity and is highly immunogenic.…”

Section: Discussionsupporting

confidence: 69%

Biochemical and genetic characterization of serologically untypable Streptococcus mutans strains isolated from patients with bacteremia

Fujiwara¹,

Nakano²,

Kawaguchi³

et al. 2001

European J Oral Sciences

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Four out of 522 streptococcal isolates from the peripheral blood of patients with bacteremia exhibited typical properties of Streptococcus mutans in terms of sucrose-dependent adhesion, expression of glucosyltransferases, fermentation profiles of sugars, the presence of surface protein antigen, and DNA-DNA hybridization. Two strains were determined as serotype f and e by immunodiffusion, whereas the other two isolates did not react with the specific antiserum to S. mutans serotype c. e. or f of the eight different serotypes of mutans streptococci. The latter two untypable isolates, however, expressed a new antigenic determinant that was different from serotype c/e/f specificity as revealed by immunodiffusion. Analysis of the cell wall polysaccharides revealed very low contents of glucose in the untypable isolates. Furthermore, Southern blot analysis demonstrated that the untypable strains lacked at least one gene corresponding to a glucose-adding enzyme. These results indicate that the serologically untypable nature is due to the loss of glucosidic residue from the serotype-specific polysaccharide antigens of S. mutans.

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Section: Discussionsupporting

confidence: 69%

Biochemical and genetic characterization of serologically untypable Streptococcus mutans strains isolated from patients with bacteremia

Fujiwara¹,

Nakano²,

Kawaguchi³

et al. 2001

European J Oral Sciences

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“…Cellular proteins of 0.5 ml of denatured cell suspension were separated by SDS–PAGE using an 8 cm wide sample slot and Western‐blotted onto nitrocellulose (Mini‐Protean II gel apparatus, Bio‐Rad). After blocking, incubation with 20 ml of 1:400 diluted serum PRT and washing, bound antibody was eluted from the membrane [17]. Duplicate Western blots of E. coli XLOLR/pSsaA and cellular and supernatant proteins recovered from both S. epidermidis NCTC 11047 and S. aureus NCTC 6571 were immunoblotted with serum PRT or the eluted anti‐SsaA antibody.…”

Section: Methodsmentioning

confidence: 99%

Identification of a novel antigen fromStaphylococcus epidermidis

Lang

Livesley

Lambert

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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A genomic DNA library of Staphylococcus epidermidis NCTC 11047 was constructed, using the Lambda Zap Express cloning vector, and screened with serum collected from a patient with S. epidermidis endocarditis. Sequence analysis of a 30 kDa cloned protein, termed staphylococcal secretory antigen, SsaA, identified a novel protein not previously reported in S. epidermidis. SsaA showed strong homology with two other staphylococcal proteins: SceB from Staphylococcus carnosus and a staphyloxanthin biosynthesis protein from Staphylococcus aureus. Further investigation revealed SsaA to be a highly antigenic protein that was expressed in vivo and could be recovered from whole cells and from the culture supernatant. A combination of Western blot analysis and PCR screening identified SsaA or a homologue in 103/103 staphylococcal strains. SsaA-like genes were not detected in other Gram-positive bacteria of medical importance or a number of Gram-negative organisms. Elevated anti-SsaA IgG antibody levels were detected in sera of five patients with S. epidermidis endocarditis but not in patients with other S. epidermidis infections, endocarditis of other aetiologies or patients with no evidence of infection. The expression of SsaA during episodes of S. epidermidis endocarditis suggests a virulence role specific to the pathogenesis of this infectious disease.

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“…Dental caries is one of the most prevalent infectious diseases in humans [1,2] and has been associated with major clinical complications, such as infective endocarditis [3][4][5]. The World Oral Health Report 2003 [6], published by the World Health Organization, indicates that dental caries is a major health problem in most industrialized countries, affecting children, adolescents, and adults.…”

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confidence: 99%

Oral Therapeutic Vaccination withStreptococcus sobrinusRecombinant Enolase Confers Protection against Dental Caries in Rats

et al. 2009

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Dental caries is among the more prevalent chronic human infections for which an effective human vaccine has not yet been achieved. Enolase from Streptococcus sobrinus has been identified as an immunomodulatory protein. In the present study, we used S. sobrinus recombinant enolase (rEnolase) as a target antigen and assessed its therapeutic effect in a rat model of dental caries. Wistar rats that were fed a cariogenic solid diet on day 18 after birth were orally infected with S. sobrinus on day 19 after birth and for 5 consecutive days thereafter. Five days after infection and, again, 3 weeks later, rEnolase plus alum adjuvant was delivered into the oral cavity of the rats. A sham-immunized group of rats was contemporarily treated with adjuvant alone. In the rEnolase-immunized rats, increased levels of salivary IgA and IgG antibodies specific for this recombinant protein were detected. A significant decrease in sulcal, proximal enamel, and dentin caries scores was observed in these animals, compared with sham-immunized control animals. No detectable histopathologic alterations were observed in all immunized animals. Furthermore, the antibodies produced against bacterial enolase did not react with human enolase. Overall, these results indicate that rEnolase could be a promising and safe candidate for testing in trials of vaccines against dental caries in humans.

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Cloning and sequencing the endocarditis immunodominant antigen of Streptococcus sobrinus strain MUCOB 263

Cited by 15 publications

References 2 publications

Biochemical and genetic characterization of serologically untypable Streptococcus mutans strains isolated from patients with bacteremia

Biochemical and genetic characterization of serologically untypable Streptococcus mutans strains isolated from patients with bacteremia

Identification of a novel antigen fromStaphylococcus epidermidis

Oral Therapeutic Vaccination withStreptococcus sobrinusRecombinant Enolase Confers Protection against Dental Caries in Rats

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