Cloning and sequencing of the gene encoding chitinase ChiA from Xanthomonas sp. strain AK and some properties of ChiA

Sakka, Kazuo; Kusaka, Ryo; Kawano, Akihiro; Karita, Shuichi; Sukhumavasi, Jiraporn; Kimura, Takeshi; Ohmiya, Kunio

doi:10.1016/s0922-338x(99)80001-4

Cited by 19 publications

(8 citation statements)

References 39 publications

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“…were wrongly classified as Myxobacteriales and Cythophagales , and several Lysobacter strains were wrongly assigned to Stenotrophomonas and Xanthomonas spp. (Christensen and Cook, 1978 ; Giesler and Yuen, 1998 ; Sakka et al, 1998 ; Nakayama et al, 1999 ), the importance of this genus was underestimated for a long time. The increasing number of 16S rDNA gene sequences available in public databases and the polyphasic approach to the identification of bacterial strains has led to an increase in the identification of new Lysobacter species.…”

Section: Introductionmentioning

confidence: 99%

The Lysobacter capsici AZ78 Genome Has a Gene Pool Enabling it to Interact Successfully with Phytopathogenic Microorganisms and Environmental Factors

et al. 2016

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Lysobacter capsici AZ78 has considerable potential for biocontrol of phytopathogenic microorganisms. However, lack of information about genetic cues regarding its biological characteristics may slow down its exploitation as a biofungicide. In order to obtain a comprehensive overview of genetic features, the L. capsici AZ78 genome was sequenced, annotated and compared with the phylogenetically related pathogens Stenotrophomonas malthophilia K729a and Xanthomonas campestris pv. campestris ATCC 33913. Whole genome comparison, supported by functional analysis, indicated that L. capsici AZ78 has a larger number of genes responsible for interaction with phytopathogens and environmental stress than S. malthophilia K729a and X. c. pv. campestris ATCC 33913. Genes involved in the production of antibiotics, lytic enzymes and siderophores were specific for L. capsici AZ78, as well as genes involved in resistance to antibiotics, environmental stressors, fungicides and heavy metals. The L. capsici AZ78 genome did not encompass genes involved in infection of humans and plants included in the S. malthophilia K729a and X. c. pv. campestris ATCC 33913 genomes, respectively. The L. capsici AZ78 genome provides a genetic framework for detailed analysis of other L. capsici members and the development of novel biofungicides based on this bacterial strain.

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Section: Introductionmentioning

confidence: 99%

The Lysobacter capsici AZ78 Genome Has a Gene Pool Enabling it to Interact Successfully with Phytopathogenic Microorganisms and Environmental Factors

et al. 2016

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“…A number of bacterial endochitinases have been cloned and over-expressed, using recombinant DNA technology [7][8][9][10][11], however to our knowledge, only limited information on the heterologous expression of fungal chitinases is available.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the expression of the Trichoderma harzianum ech42 gene in two isogenic clones of Escherichia coli by surface response methodology

Léon¹,

Jiménez‐Islas²,

González-Cuevas³

et al. 2004

Process Biochemistry

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“…This is the first study of both chitinase and There have been many reports that microorganisms produce chitinase. The chitinases were discovered in species of the genera, such as bacteria: Aeromonas (Lan et al, 2006), Alteronomas (Tsujibo et al, 1993), Bacillus (Rivas et al, 2000), Enterobacter (Chernin et al, 1997), Janthinobacterium (Gleave et al, 1995), Microbacterium (Sun et al, 2006), Pseudoalteromonas (Techkarnjanaruk and Goodman, 1999), Pseudomonas (Folders et al, 2001), Serratia (Gal et al, 1998), Xanthomonas (Sakka et al, 1998), Vibrio (Morimoto et al, 1993), Coccidioides (Pishko et al, 1995), Clostridium (Morimoto et al, 1997), and Streptomyces (Kawase et al, 2006); fungi: Candida (McCreath et al, 1995), Aspergillus (Xia et al, 2001), Metarhizium (Kang et al, 1998), Rhizopus (Yanai et al, 1992), Trichoderma (Hayes et al, 1994) and Saccharomyces (Carstens et al, 2003). Sphingomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Analysis of both chitinase and chitosanase produced by Sphingomonas sp. CJ-5

Zhu

Zhou

Feng

2007

J. Zhejiang Univ. - Sci. B

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A novel chitinolytic and chitosanolytic bacterium, Sphingomonas sp. CJ-5, has been isolated and characterized. It secretes both chitinase and chitosanase into surrounding medium in response to chitin or chitosan induction. To characterize the enzymes, both chitinase and chitosanase were purified by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-Sepharose Fast Flow. SDS-PAGE analysis demonstrated molecular masses of chitinase and chitosanase were 230 kDa and 45 kDa respectively. The optimum hydrolysis conditions for chitinase were about pH 7.0 and 36 degrees C, and these for chitosanase were pH 6.5 and 56 degrees C, respectively. Both enzymes were quite stable up to 45 degrees C for one hour at pH 5~8. These results show that CJ-5 may have potential for industrial application particularly in recycling of chitin wastes.

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Cloning and sequencing of the gene encoding chitinase ChiA from Xanthomonas sp. strain AK and some properties of ChiA

Cited by 19 publications

References 39 publications

The Lysobacter capsici AZ78 Genome Has a Gene Pool Enabling it to Interact Successfully with Phytopathogenic Microorganisms and Environmental Factors

The Lysobacter capsici AZ78 Genome Has a Gene Pool Enabling it to Interact Successfully with Phytopathogenic Microorganisms and Environmental Factors

Analysis of the expression of the Trichoderma harzianum ech42 gene in two isogenic clones of Escherichia coli by surface response methodology

Analysis of both chitinase and chitosanase produced by Sphingomonas sp. CJ-5

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