Cloning and sequencing of the FSH-β and LH β-subunit in the three-spined stickleback, Gasterosteus aculeatus, and effects of photoperiod and temperature on LH-β and FSH-β mRNA expression

Hellqvist, Anna; Bornestaf, Cecilia; Borg, Bertil; Schmitz, Monika

doi:10.1016/j.ygcen.2003.09.003

Cited by 38 publications

(28 citation statements)

References 22 publications

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“…Gene expression of GnRHs or GTHs in response to photoperiod manipulation has been reported in some fish species, including salmonids, pejerrey (Odontesthes bonariensis), sea bass (Dicentrarchus labrax), and threespined stickleback (Gasterosteus aculeatus) (Amano et al, 1999Hellqvist et al, 2004;Felip et al, 2008;Miranda et al, 2009). However, little is known about the orchestration of gene expression for GnRHs (GnRH3 and GnRH2), GTH subunits (GTHα, FSHβ, and LHβ), and VTG, or their association with steroidogenesis and ovarian maturation in female fishes undergoing photoperiod manipulation.…”

Section: Introductionmentioning

confidence: 99%

Effects of Shortened Photoperiod on Gonadotropin-Releasing Hormone, Gonadotropin, and Vitellogenin Gene Expression Associated with Ovarian Maturation in Rainbow Trout

Choi

Lee²,

Park

et al. 2010

Zoological Science

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Reproductive activities of salmonids are synchronized by changes in photoperiod, which control the endocrine system via the brain-pituitary-gonadal axis. Gonadotropin-releasing hormone (GnRH) in the brain regulates synthesis and release of the pituitary gonadotropins (GTHs; FSH and LH). FSH and LH in turn stimulate the production of sex steroids for oocyte growth and maturation-Inducing steroid hormones for oocyte maturation and ovulation, respectively, in female salmonids. To clarify effects of long-term photoperiod manipulations on the reproductive activity of salmonids from early recrudescence to ovulation, we Investigated the gene expression profiles of GnRH, GTHs, and vitellogenin (VTG), and plasma sex steroids in female rainbow trout (Oncorhynchus mykiss). In addition, the percentages of eyed embryos and hatched alevins were examined together with the number of ovulated eggs to evaluate the effects of photoperiod regimes on egg quality. During late summer, the mRNA levels of GnRHs, GTHalpha, and LHbeta, and the plasma level of a maturational steroid (17alpha,20beta-dihydroxy-4-pregnen-3-one; 17,20beta-P) were significantly elevated by a gradually shortened photoperiod under constant temperature, in accordance with accelerated sexual maturation. The percentages of eyed embryos and hatched alevins from fish ovulated in August were comparable to those of control fish observed in December. These results clearly indicate that syntheses of GnRHs, LH, VTG, and 17,20beta-P are effectively accelerated by a programmed long-short photoperiod regime in early recrudescent female rainbow trout, without a marked deterioration in egg quality.

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Section: Introductionmentioning

confidence: 99%

Effects of Shortened Photoperiod on Gonadotropin-Releasing Hormone, Gonadotropin, and Vitellogenin Gene Expression Associated with Ovarian Maturation in Rainbow Trout

Choi

Lee²,

Park

et al. 2010

Zoological Science

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“…It is well known that temperature is one of the main environmental factors that influence the reproductive system in fish and that even small changes in this variable can cause alterations in the reproductive cycle, hormonal synthesis, gametogenesis, and reproductive fitness (Van der Kraak and Pankhurst 1997;Shimizu 2003;Hellqvist et al 2004). The increases noted in the water temperature of many rivers, lagoons, and oceans, which are considered to be consequences of global warming, have already started to affect life history, behavior, and reproduction in fish (Myrick and Cech 2000;Lass and Spaak 2003;Roessig et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In spite of the knowledge that environmental changes profoundly affect the reproductive function in fish (Bromage et al 2001) and that this is achieved by alterations in the brainpituitary-gonad axis, the linkage between the environment on the one hand and the neuroendocrine cascade controlling reproduction on the other remains unclear (Amano et al 2004;Hellqvist et al 2004). At the endocrinological level, fish reproduction is regulated by gonadotropin-releasing hormones (GnRHs) that control the synthesis and release of gonadotropins (GtHs).…”

Section: Introductionmentioning

confidence: 99%

High Water Temperatures Impair the Reproductive Ability of the Pejerrey Fish Odontesthes bonariensis: Effects on the Hypophyseal‐Gonadal Axis

Soria

Strüssmann²,

Miranda³

2008

Physiological and Biochemical Zoology

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The aim of this research was to study how high water temperatures impair the reproductive activity of pejerrey Odontesthes bonariensis, an inland-water atherinopsid fish species from the pampasic region of Argentina. Adult pejerrey of both sexes were kept under a control-temperature regime (19 degrees C) and two experimental temperatures (23 degrees and 27 degrees C) for 8 d. The effect of elevated temperature on the pituitary-gonad axis was analyzed in terms of the expression of gonadotropin (GtH) subunits: follicle-stimulating hormone beta, luteinizing hormone beta (LH-beta), glycoprotein hormone alpha, and GtH receptors FSH-R and LH-R by semiquantitative reverse transcriptase polymerase chain reaction, plasma levels of sexual steroids by radioimmunoassay, and reproductive status by gonadal histology. The results of this work clearly indicate that short periods of exposure to high water temperatures disrupt pejerrey reproduction. This effect was observed in spawning activity, at the histological level, and in the reduction of plasma estradiol in females and testosterone in males. The mRNA levels of GtH subunits and GtH receptors generally decreased in proportion to the increase in temperature for both sexes. However, the differences between groups were statistically significant only for LH-beta and for FSH-R expression in pejerrey females. Thus, the gonads of pejerrey appear to be the primary target of high water temperature. Analysis of the air temperature in this region over the past 40 yr indicated an increase of 1.74 degrees C in mean annual temperature. This increase, coupled with the mechanism of high-temperature sensitivity shown in this study, may be one of the reasons for the decline in pejerrey populations observed in this region over recent decades.

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“…The environmental effect on hormones that regulate reproduction and growth in fish has been studied extensively, focusing particularly on photoperiod [1,2], temperature [3][4][5][6][7][8][9][10], water quality [11], salinity [12,13] and diet [14].…”

Section: Introductionmentioning

confidence: 99%

Underground water affects sexual behavior and gene expression of hormones related to reproduction in blue gourami males

Degani¹,

Lévy²

2013

ABC

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This study examined the effect of underground water on reproduction-and growth-related hormones in blue gourami males under non-reproductive and reproductive conditions. An increase in the percentage of males building nests under the highest percentage of underground water were compared to fish that maintained a lower percentage of underground water in the first two days. The % Gonado-somatic index (GSI) of males building nests was higher than nonreproductively active males in water containing the lowest concentration of underground water. In nonreproductively active males, brain gonadotropin releasing hormone 1 (GnRH1) and pituitary β subunit of gonadotropins (GtHs) and prolactin (PRL) mRNA levels were significantly higher in males maintained in underground water. In reproductively active males, mRNA levels of brain GnRH1, gonadotropin releasing hormone 3 (GnRH3) and pituitary PRL mRNA levels were significantly higher than males maintained in underground water. Thus, it is suggested that underground water with high salinity and conductivity levels affects the gene expression of reproduction-related hormones; in reproductively active males, it shortened the duration of nest-building by blue gourami males.

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Cloning and sequencing of the FSH-β and LH β-subunit in the three-spined stickleback, Gasterosteus aculeatus, and effects of photoperiod and temperature on LH-β and FSH-β mRNA expression

Cited by 38 publications

References 22 publications

Effects of Shortened Photoperiod on Gonadotropin-Releasing Hormone, Gonadotropin, and Vitellogenin Gene Expression Associated with Ovarian Maturation in Rainbow Trout

Effects of Shortened Photoperiod on Gonadotropin-Releasing Hormone, Gonadotropin, and Vitellogenin Gene Expression Associated with Ovarian Maturation in Rainbow Trout

High Water Temperatures Impair the Reproductive Ability of the Pejerrey Fish Odontesthes bonariensis: Effects on the Hypophyseal‐Gonadal Axis

Underground water affects sexual behavior and gene expression of hormones related to reproduction in blue gourami males

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