Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)

Abstract

cDNA clones having a nucleotide sequence encoding a human monocyte chemotactic and activating factor (MCAF) were isolated and sequenced. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. The amino acid sequence of MCAF showed 25-55% homology with other members of an inducible cytokine family, including macrophage inflammatory protein and some putative polypeptide mediators known as JE, LD78, RANTES and TCA-3. This suggests that MCAF is a member of family of factors involved in immune and inflammatory responses.

The chemokine system: tuning and shaping by regulation of receptor expression and coupling in polarized responses

“…Thus, in the mid-1980s, this gene (JE) was in search for function and a monocyte-specific attractant was waiting for molecular definition. In 1989, CCL2/ MCP-1 was successfully purified from supernatants of a human glioma (13), a human monocytic leukemia (14), and a human sarcoma (15-17): sequencing and molecular cloning revealed its relationship with the long-known JE gene (18)(19)(20).…”

Expression of monocyte chemotactic and activating factor in rheumatoid arthritis. regulation of its production in synovial cells by interleukin‐1 and tumor necrosis factor

“…A novel cytokine, monocyte chemotactic and activating factor (MCAF) or monocyte chemoattractant protein 1 (MCP-l), has been recently identified (7,8). MCAF is a peptide that consists of 76 amino acids and possesses structural homology with a group of putative polypeptide mediators known as macrophagederived inflammatory protein 1 (MIP-l), LD-78, RANTES, and JE.…”

Monocytic Chemotactic Protein-1 in Physiology and Medicine (Review of Literature)

“…В геноме человека CCL2 и многие дру гие СС хемокины находятся на хромосоме 17 (17q11.2 в21.1) [16,33] На клеточной поверхности выделяют два вида рецепторов, которые связывают CCL2 -CCR2 и CCR4. Результат активации рецептора MCP 1 может быть как проинфламаторным (опосредован антигенпрезентующими и Т клетками), так и антиинфламаторным (опо средован регуляторными T клетками) [50].…”