Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA

Sequence polymorphism in the 5'NTR and in the P1 coding region of potato virus Y genomic RNA

“…The acronyms, geographical origin, references and symptoms caused on Nicotiana tabacum by the PVY isolates studied in this paper are shown in Table 1. We have intentionally used the terms Thole et al (1993); c, Chrzanowska (1991);d, Robaglia et al (1989); e, Thornbury et al (1990);f, this work; g, Gooding & Tolin (1973);h, K. Grbr6 Srlassi6 (personal communication);i, Grbr6 Srlassi6 et al (1985). …”

“…The complete nucleotide sequence of several members of the group is already known (Allison et al, 1986;Domier et al, 1986;Robaglia et al, 1989;Lain et al, 1989;Maiss et al, 1989;Teycheney et al, 1989;Johansen et al, 1991 ; * Author for correspondence. Fax +33 1 30 83 31 95. e-mail robaglia@oxygene.versailles.inra.fr Nicolas & Lalibertr, 1992;Yeh et al, 1992;Jayaram et al, 1992;Vance et al, 1992;Thole et al, 1993;Palkovics et al, 1993). A single ORF is translated into a polyprotein of more than 3000 amino acids, which is then proteolytically processed by three viral proteases into functional proteins (Carrington & Dougherty, 1987;Carrington et al, 1989;Verchot et al, 1991).…”

“…Nucleotide sequence analysis of isolates PVY-Fr (Robaglia et al, 1989) and PVY-H (Thole et al, 1993), which have been completely sequenced, shows that the degree of sequence similarity differs across the genome. The overall nucleotide identity between these two isolates is 88"5 % while the 5' non-translated region (5"NTR) and the adjacent P1 coding region have only 70.3% and 72.6% identity, respectively.…”

“…We have thus chosen to quantify sequence polymorphism of PVY isolates in this particular region. As 5'NTR and P1 coding region sequences were available for only four PVY isolates (Robaglia et al, 1989;Thole et al, 1993;Thornbury et al, 1990), we determined the nucleotide sequence of the 5'NTR for ten PVY isolates and of the P1 coding region for eight isolates. Analysis of these data allowed a clustering of PVY isolates and highlighted nucleic acid and amino acid motifs which could have functional significance in the 5'NTR and in the P1 coding region.…”

No recombination detected in artificial potyvirus mixed infections and between potyvirus derived transgenes and heterologous challenging potyviruses

“…The infectious full-length clone p35PPV-NAT of the non-aphid transmissible strain of PPV (Maiss et al, 1992) as well as wildtype virus isolates of TVMV (PV-0253, DSMZ, Germany), PVA (SCRI collection), PVY-O (SCRI collection), PPV-SK68 (Palkovics et al, 1993) and PVY-H (Thole et al, 1993) were used in the respective experiments.…”