Diversity, Characterisation and Classification of PVY

Glais, Laurent; Du, Baoheng; Lacomme, Christophe

doi:10.1007/978-3-319-58860-5_3

Cited by 15 publications

(20 citation statements)

References 72 publications

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“…Recombination clearly played a significant role in the evolutionary history of PVY [10,17,35]. Recombination, together with mutation, increases the genetic variability of the given taxa, which can result in higher viral fitness or survival of the virus population in a previously nonviable environment, sometimes leading to the emergence of new resistance-breaking strains [36,37].…”

Section: Discussionmentioning

confidence: 99%

“…All these phytosanitary actions require access to early, sensitive, and specific detection [9]. Although ELISA and RT-PCR-based techniques provide robust tools for the diagnosis of plant viruses, the large genetic diversity found in many viral species can impose severe constraints on the design and performance of some detection systems [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Glasa

Hančinský

Šoltýs

et al. 2021

Plants

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In recent years, high throughput sequencing (HTS) has brought new possibilities to the study of the diversity and complexity of plant viromes. Mixed infection of a single plant with several viruses is frequently observed in such studies. We analyzed the virome of 10 tomato and sweet pepper samples from Slovakia, all showing the presence of potato virus Y (PVY) infection. Most datasets allow the determination of the nearly complete sequence of a single-variant PVY genome, belonging to one of the PVY recombinant strains (N-Wi, NTNa, or NTNb). However, in three to-mato samples (T1, T40, and T62) the presence of N-type and O-type sequences spanning the same genome region was documented, indicative of mixed infections involving different PVY strains variants, hampering the automated assembly of PVY genomes present in the sample. The N- and O-type in silico data were further confirmed by specific RT-PCR assays targeting UTR-P1 and NIa genomic parts. Although full genomes could not be de novo assembled directly in this situation, their deep coverage by relatively long paired reads allowed their manual re-assembly using very stringent mapping parameters. These results highlight the complexity of PVY infection of some host plants and the challenges that can be met when trying to precisely identify the PVY isolates involved in mixed infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Glasa

Hančinský

Šoltýs

et al. 2021

Plants

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“…PVY N:O , PVY NTN (N-tuber necrotic), and PVY N-Wi (N-Wilga). 22,23,44 PVY infection increases the number of undersized tubers. Foliar symptoms include mosaic (chlorotic patches; Figure 2a) that may be severe or mild and hardly detectable.…”

Section: Potato Virus Ymentioning

confidence: 99%

“…20 PVY has been a challenge worldwide during the past 20 years due to the emergence of recombinant PVY variants. 22,23 Both viruses result in dramatic yield and quality losses. 21,24 Infection levels are increased by planting infected tubers, leading to increased infection levels over successive generations.…”

Section: Introductionmentioning

confidence: 99%

Potato virus Y and Potato leafroll virus management under climate change in sub-Saharan Africa

Krüger

Waals

2020

S. Afr. J. Sci.

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Potato has increased in importance as a staple food in sub-Saharan Africa, where its production is faced with a multitude of challenges, including plant disease development and spread under changing climatic conditions. The economically most important plant viruses affecting potatoes globally are Potato virus Y (PVY) and Potato leafroll virus (PLRV). Disease management relies mostly on the use of insecticides, cultural control and seed certification schemes. A major obstacle in many sub-Saharan Africa countries is the availability of disease-free quality seed potatoes. Establishment and implementation of quality control through specialised seed production systems and certification schemes is critical to improve seed potato quality and reduce PVY and PLRV sources. Seed could be further improved by breeding virus-resistant varieties adapted to different environmental conditions combined with management measures tailored for smallholder or commercial farmers to specific agricultural requirements. Innovative technologies – including more sensitive testing, remote sensing, machine learning and predictive models – provide new tools for the management of PVY and PLRV, but require support for adoption and implementation in sub-Saharan Africa.

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“…It was shown that the avirulence factor of the PVY virus is the helper component proteinase (HC-Pro) cistron of PVY, while Nx-mediated hypersensitivity and Rx-mediated extreme resistance were elicited by different subunits of coat protein (CP) of PVX [79]. CP of a virus as well as viral RNA, the so-called pathogen-associated molecular patterns (PAMPs), are recognized by plant cell receptors, leading to the development of defense responses in plants [80], including rapid generation of reactive oxygen species (ROS), changes in the content of phytohormones, synthesis of other metabolites, as well as the induction of local and systemic expression of defense genes [81].…”

Section: Virus Recognition and Systemic Resistance In Plantsmentioning

confidence: 99%

Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms

Максимов

Sorokan

Бурханова

et al. 2019

Plants

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Plant viruses are globally responsible for the significant crop losses of economically important plants. All common approaches are not able to eradicate viral infection. Many non-conventional strategies are currently used to control viral infection, but unfortunately, they are not always effective. Therefore, it is necessary to search for efficient and eco-friendly measures to prevent viral diseases. Since the genomic material of 90% higher plant viruses consists of single-stranded RNA, the best way to target the viral genome is to use ribonucleases (RNase), which can be effective against any viral disease of plants. Here, we show the importance of the search for endophytes with protease and RNase activity combined with the capacity to prime antiviral plant defense responses for their protection against viruses. This review discusses the possible mechanisms used to suppress a viral attack as well as the use of local endophytic bacteria for antiviral control in crops.

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Diversity, Characterisation and Classification of PVY

Cited by 15 publications

References 72 publications

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Potato virus Y and Potato leafroll virus management under climate change in sub-Saharan Africa

Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms

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