Cloning and sequencing of Duck circovirus (DuCV)

Hattermann, Kim; Schmitt, Cornelia; Soike, Dirk; Mankertz, Annette

doi:10.1007/s00705-003-0181-y

Cited by 133 publications

(110 citation statements)

References 19 publications

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“…The first PCR step capitalized on the probability that nucleotide sequences present in the CaCV genome, which were highly conserved when isolates of PiCV, BFDV and CaCV were compared, would also occur in the genomes of circoviruses that infect finches and gulls and that, as such, PCR amplification of circovirus amplicons would be facilitated. Earlier investigations used degenerate primers, selected after comparison of the PCV1 and BFDV genome sequences, to amplify small regions of the Rep genes of PiCV, GoCV and CaCV (Phenix et al ., 2001;Todd et al ., 2001b;Hattermann et al ., 2003). This is the first reported use of the CAN-1F/CAN-IR primer pair, and the success achieved suggests that these primers may be useful for detecting other avian circoviruses that are more closely related to BFDV, PiCV and CaCV.…”

Section: Discussionmentioning

confidence: 99%

“…Infections with BFDV and PiCV are both associated with lymphoid depletion and immunosuppression, which appears to increase the severity of secondary infections. Circoviruses have been detected in farmed geese and ducks with growth and feathering problems but the clinico-pathological significance and economic importance of circovirus infections of these birds remains unknown (Hattermann et al ., 2003;Soike et al ., 1999Soike et al ., , 2004 Little is known about the roles of circoviruses in causing disease in passerine species such as canaries and finches. Circoviruses have been reported to cause a condition known as black spot in neonatal canaries in Europe (Goldsmith, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of novel circoviruses from finch and gull

et al. 2007

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The purpose of this study was to molecularly characterize circoviruses that infect finches and gulls. Circovirus-specific DNAs were isolated using polymerase chain reaction methods from bursa of Fabricius tissues from a Gouldian finch (Chloebia gouldiae) and a herring gull (Larus argentatus) that were known to be circovirus-infected. Nucleotide sequence determination and analysis of cloned genomic DNAs showed that these circoviruses represented novel members of the genus Circovirus of the family Circoviridae, and have been tentatively named Finch circovirus (FiCV) and Gull Circovirus (GuCV). Both new circoviruses shared genome organizational features with previously characterized circoviruses, such that both contained two major, inversely-arranged open reading frames encoding the putative replication-associated and capsid proteins, and both contained a potential stemÁloop and nonanucleotide motif. Phylogenetic analyses based on genome nucleotide sequences and involving the seven additional genus members indicated that FiCV and GuCV were more closely related to canary circovirus, beak and feather disease virus and pigeon circovirus, and that FiCV and canary circovirus were the most closely related avian circoviruses. Pairwise comparisons showed that the capsid proteins of FiCV and GuCV shared highest amino acid identity values with those of canary circovirus (62.0%) and pigeon circovirus (40.6%), respectively. The 5? intergenic region of GuCV was longer (207 nucleotides) and contained more direct and inverse repeated sequences than those of other circoviruses, while the 3? intergenic region of FiCV was notable in being longer (307 nucleotides) than its counterparts in other circoviruses and in containing two long repeats of 77 nucleotides.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of novel circoviruses from finch and gull

et al. 2007

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“…Viruses from the genus Cyclovirus have been found in humans and chimpanzees 2. Circoviruses have a wide host range, including human,2, 3 birds,4, 5 pigs,6, 7 dogs,8 and cattle 9, 10. Before to 2015, porcine circovirus 1 (PCV1) and PCV2 were considered the only types of porcine circoviruses.…”

Section: Introductionmentioning

confidence: 99%

Origin, Genetic Diversity, and Evolutionary Dynamics of Novel Porcine Circovirus 3

et al. 2018

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Porcine circovirus 3 (PCV3) is a novel virus associated with acute PDNS (porcine dermatitis and nephropathy syndrome)‐like clinical signs identified by metagenomic sequencing from swine. Its high occurrence may pose a potential threat to the swine industry worldwide. The processes resulting in the emergence and spread of PCV3 remain poorly understood. Herein, the possible origin, genotypes, and evolutionary dynamics of PCV3 based on available genomic sequences are determined. The closest ancestor of PCV3 is found to be within the clade 1 bat CVs. Using different phylogenetic methods, two major genotypes are identified, PCV3a and PCV3b. It is found that the effective population size of PCV3 increased rapidly during late 2013 to early 2014 and this is associated with the diversification of PCV3a and PCV3b. A relatively high effective reproductive number (Re) value and higher evolutionary rate were found compared to other single‐stranded DNA viruses, and positive selection on codons 122 and 320 (24 of ORF2) is identified. It is hypothesized that this, together with the prediction of a potential change of an antigenic epitope at position 320, might have allowed PCV3 to escape from the host immune response. Overall, this study has important implications for understanding the ongoing PCV3 cases worldwide and will guide future efforts to develop effective preventive and control measures.

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“…A circovirus-like infection of ducks was first described in Germany in two female 6-week-old mulard ducks from a German farm (Hattermann et al ., 2003;Soike et al ., 2004). The birds showed a feathering disorder, poor body condition and low weight for their age.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests

et al. 2005

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Development of the first conventional and real-time polymerase chain reaction (PCR) tests for the diagnoses of duck circovirus (DuCV) infections is described. Both tests amplified a 230 bp fragment specific to the DuCV Rep gene. Although both tests had the same detection limit (13 )/10 3 target DNA/ml) when the target DNA was diluted in water, the detection limit of the real-time test (13 )/10 4 target DNA/ml) was 10-fold less than the conventional test (13)/10 5 target DNA/ml) when the amplifications were performed in the presence of cellular DNA. Using the conventional PCR test, DuCV DNA was detected in 85 (84%) of 101 bursa of Fabricius samples from dead or sick ducks, aged between 1 and 12 weeks, and in samples from 35 (94%) of 37 flocks. Application of the SYBR Green-based real-time PCR test to 54 selected bursa of Fabricius samples indicated that more samples were positive by real-time PCR than by conventional PCR, allowed the numbers of genome copies to be estimated and showed that some bursa of Fabricius samples contained over 10 13 genome copies/g tissue. Although DuCV infections were detected in birds aged from 1 to 12 weeks, higher virus DNA levels were detected in ducks aged older than 5 weeks than in ducks younger than 5 weeks. An in situ hybridization method for the detection of DuCV in histological samples was also developed. Additional work is required to determine the clinicopathological significance of DuCV infections.

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Cloning and sequencing of Duck circovirus (DuCV)

Cited by 133 publications

References 19 publications

Molecular characterization of novel circoviruses from finch and gull

Molecular characterization of novel circoviruses from finch and gull

Origin, Genetic Diversity, and Evolutionary Dynamics of Novel Porcine Circovirus 3

Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests

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