Molecular characterization of novel circoviruses from finch and gull

Abstract

The purpose of this study was to molecularly characterize circoviruses that infect finches and gulls. Circovirus-specific DNAs were isolated using polymerase chain reaction methods from bursa of Fabricius tissues from a Gouldian finch (Chloebia gouldiae) and a herring gull (Larus argentatus) that were known to be circovirus-infected. Nucleotide sequence determination and analysis of cloned genomic DNAs showed that these circoviruses represented novel members of the genus Circovirus of the family Circoviridae, and have been tentatively named Finch circovirus (FiCV) and Gull Circovirus (GuCV). Both new circoviruses shared genome organizational features with previously characterized circoviruses, such that both contained two major, inversely-arranged open reading frames encoding the putative replication-associated and capsid proteins, and both contained a potential stemÁloop and nonanucleotide motif. Phylogenetic analyses based on genome nucleotide sequences and involving the seven additional genus members indicated that FiCV and GuCV were more closely related to canary circovirus, beak and feather disease virus and pigeon circovirus, and that FiCV and canary circovirus were the most closely related avian circoviruses. Pairwise comparisons showed that the capsid proteins of FiCV and GuCV shared highest amino acid identity values with those of canary circovirus (62.0%) and pigeon circovirus (40.6%), respectively. The 5? intergenic region of GuCV was longer (207 nucleotides) and contained more direct and inverse repeated sequences than those of other circoviruses, while the 3? intergenic region of FiCV was notable in being longer (307 nucleotides) than its counterparts in other circoviruses and in containing two long repeats of 77 nucleotides.

Multiple Diverse Circoviruses Infect Farm Animals and Are Commonly Found in Human and Chimpanzee Feces

“…The approximately 400-bp amplicons were sequenced, and translated amino acids were aligned using the homologous region of the putative Rep-associated protein of CAV as the outgroup. The derived phylogenetic tree was consistent with prior analyses based on the complete Rep protein sequences and on the complete genome of animal circoviruses (12,16,22,38,39). A densely populated cluster of Rep sequences (including that of the Cyclovirus prototype genome) was labeled cycloviruses in Fig.…”

“…Sequence alignment of the putative Rep protein of CyCV1-PK5006 with that of known species in the genus Circovirus identified several highly conserved amino acids motifs (see Fig. S1 in the supplemental material) (15,31,38). Frequent detection and analysis of circovirus-like Rep sequences in human and animal specimens.…”

“…Circoviruses infect several avian groups, including parrots, pigeons, gulls, anserids (ducks, geese, and swans), and numerous passerines (ravens, canaries, finches, and starlings) (12,15,16,22,26,31,35,38,39). Avian circoviruses have been associated with a variety of symptoms, including developmental abnormalities, lymphoid depletion, and immunosuppression (22,26,28,35,39).…”

<b>Caracterização molecular de bornavírus, poliomavírus e circovírus em aves de cativeiro, vida livre e criação comercial</b>

“…Uma dificuldade encontrada foi os diversos tipos de circovírus que afetam as aves, que incluem, além da doença do bico e das penas de psitacídeos (BFDV) e anemia infecciosa das galinhas (CAV) o circovírus de pombos (PiCV), circovírus de canários (CaCV), circovírus de patos (DuCV), circovírus de ganso (GoCV) (TODD et al, 2007). Neste trabalho, foram testados primers apenas para dois destes vírus (BFDV e CAV), esta escolha foi feita baseada na importância econômica destas doenças para a avicultura comercial e doméstica.…”

“…A doença mais comum entre as espécies silvestres é a doença do bico e das penas de psitacídeos, descrita pela primeira vez em 1975 (LATIMER et al, 1990). Entretanto, o circovírus afeta pombos (PiCV), galinhas (anemia infecciosa das galinhas), patos, gansos, cisnes e canários (SOIKE et al, 2004;TODD et al, 2007).…”