Cloning and sequencing of a gene cluster for the Meta-cleavage pathway of aniline degradation in Acinetobacter sp. strain YAA

Elucidation of the 4-Hydroxyacetophenone Catabolic Pathway in Pseudomonas fluorescens ACB

“…Based on the hydroquinone degradation experiments reported above, HapE is assigned to be an NAD ϩ -dependent dehydrogenase (EC 1.2.1.61) that converts 4-hydroxymuconic semialdehyde to maleylacetate. Interestingly, HapE shows 37 to 43% sequence identity with 2-hydroxymuconic semialdehyde dehydrogenases, which are involved in the meta-cleavage pathways of catechol degradation (66,80). There is no other sequence of a 4-hydroxymuconic semialdehyde dehydrogenase known.…”

Mechanism of 4-Nitrophenol Oxidation in Rhodococcus sp. Strain PN1: Characterization of the Two-Component 4-Nitrophenol Hydroxylase and Regulation of Its Expression

“…First, to use as a reporter gene, a DNA fragment of approximately 1.0 kb containing a catechol 2,3-dioxygenase (C23O) gene (atdB) of Acinetobacter sp. strain YAA (42,45) was amplified by PCR using KOD DNA polymerase (Toyobo, Osaka, Japan), which produces blunt ends on the amplified fragment. The PCR mixture contained 0.…”

“…The PCR mixture contained 0. (42,45) was inserted into the StuI site and the EcoRV site of pKPN3 as a reporter gene to construct pKPN32 and pKPN35, respectively. For the disruption of nphR, a tetracycline resistance gene (tet) from pBR322 (4) was introduced into the EcoRV site of pKPN32 to construct pKPN33.…”

Cloning and Functional Analysis of Aniline Dioxygenase Gene Cluster, fromFrateuriaSpecies ANA-18, That Metabolizes Anilineviaanortho-Cleavage Pathway of Catechol

“…YAA. 7,8) The ORFs were organized as well as the alignments of the tdn and atd genes. On the basis of these results, it was concluded that ORF1 to 5 and ORF6 were tdnQTA1A2B and tdnR, respectively, encoding AD components and a LysR-type regulator.…”

“…[2][3][4][5] Furthermore, genes encoding aniline dioxygenases (AD), catalyzing the conversion of aniline to catechol, have been cloned from Pseudomonas putida UCC22, 6) Acinetobacter sp. YAA, 7,8) and Pseudomonas sp. AW-2, 9) which metabolize aniline via the meta-cleavage pathway.…”