Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Arthrobacter sp. strain SU

Schmitz, A; Gartemann, Karl-Heinz; Fiedler, J; Grund, E.; Eichenlaub, Rudolf

doi:10.1128/aem.58.12.4068-4071.1992

Cited by 76 publications

(27 citation statements)

References 23 publications

(26 reference statements)

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“…4). The 4HBA-CoA produced from 4CBA by the catalysis of FcbA and FcbB was converted into the final product 4HBA because the thioester hydrolysis of 4HBA-CoA into 4HBA could occur by the action of thioesterases in E. coli as reported by Schmitz et al (1992). This result along with our bioinformatic analysis confirms that the gene products are responsible for the hydrolytic dechlorination of 4CBA.…”

Section: Expression Of Fcbba and Transformation Of 4cbasupporting

confidence: 78%

“…1a (Chang et al, 1992). The genes and enzymes for hydrolytic dechlorination of 4CBA-CoA have been characterized only from aerobic organisms (Chang et al, 1992;Schmitz et al, 1992;Chae et al, 2000;Zhou et al, 2004). Although genes for hydrolytic dehalogenation of 4CBA have not been reported from anaerobic microorganisms, it has been presented by Coschigano et al (1994) that the genes from aerobic organisms can be expressed in denitrifying strains and allow them to grow on 4CBA under anaerobic denitrifying conditions.…”

Section: Resultsmentioning

confidence: 99%

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Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium

Chae

Song

Zylstra

2008

FEMS Microbiology Letters

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A metagenomic approach was taken to investigate the genetic basis for the ability of an anaerobic consortium to grow on either 4-chlorobenzoate or 4-bromobenzoate under denitrifying conditions. Degenerate PCR primers were designed for the family of 4-chlorobenzoyl-CoA dehalogenase genes. The primers were utilized to screen a metagenome library and two overlapping clones were identified which yield a PCR product. The complete sequence of one metagenome clone was determined and genes encoding 4-chlorobenzoyl-CoA ligase (FcbA) and 4-chlorobenzoyl-CoA dehalogenase (FcbB) were identified. Analysis of the ORFs present in the nucleotide sequence suggests that the metagenome clone originated from an uncultured denitrifying microorganism belonging to the Betaproteobacteria. Interestingly, unlike similar gene clusters reported in aerobes, a gene encoding 4-hydroxybenzoyl-CoA thioesterase was not present in the gene cluster. This suggests that 4-hydroxybenzoyl-CoA is further degraded via the anaerobic reduction pathway in the corresponding microorganism instead of through thioester hydrolysis to yield 4-hydroxybenzoate.

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Section: Expression Of Fcbba and Transformation Of 4cbasupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium

Chae

Song

Zylstra

2008

FEMS Microbiology Letters

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“…On the other hand, a number of CBA-degrading bacteria that remove chlorine prior to oxidation of the aromatic ring, funneling the resulting nonchlorinated benzoate or catechol into central metabolic pathways, have been isolated (13,14,35,42,46,50,51). While no dehalogenation of chloropentadiene has been documented so far (9), several CBA dehalogenation genes have been cloned and characterized (18,33,37,38,39,43,44), and these are potentially useful for constructing organisms that would grow on PCBs.…”

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confidence: 99%

Construction and Characterization of Two Recombinant Bacteria That Grow on ortho - and para -Substituted Chlorobiphenyls

Hrywna

Мальцева

et al. 1999

Appl Environ Microbiol

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Cloning and expression of the aromatic ring dehalogenation genes in biphenyl-growing, polychlorinated biphenyl (PCB)-cometabolizing Comamonas testosteroni VP44 resulted in recombinant pathways allowing growth on ortho-and para-chlorobiphenyls (CBs) as a sole carbon source. The recombinant variants were constructed by transformation of strain VP44 with plasmids carrying specific genes for dehalogenation of chlorobenzoates (CBAs). Plasmid pE43 carries the Pseudomonas aeruginosa 142 ohb genes coding for the terminal oxygenase (ISP OHB ) of the ortho-halobenzoate 1,2-dioxygenase, whereas plasmid pPC3 contains the Arthrobacter globiformis KZT1 fcb genes, which catalyze the hydrolytic para-dechlorination of 4-CBA. The parental strain, VP44, grew only on low concentrations of 2-and 4-CB by using the products from the fission of the nonchlorinated ring of the CBs (pentadiene) and accumulated stoichiometric amounts of the corresponding CBAs. The recombinant strains VP44(pPC3) and VP44(pE43) grew on, and completely dechlorinated high concentrations (up to 10 mM), of 4-CBA and 4-CB and 2-CBA and 2-CB, respectively. Cell protein yield corresponded to complete oxidation of both biphenyl rings, thus confirming mineralization of the CBs. Hence, the use of CBA dehalogenase genes appears to be an effective strategy for construction of organisms that will grow on at least some congeners important for remediation of PCBs.

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“…A hydratase type reaction for removing halogen substituents from an unsaturated carbon atom is more clearly established in Pseudomonas CBS3 degrading 4-chlorobenzoic acid [44][45][46][47][48]. This aromatic compound has to be activated via the formation of a thioester bond with coenzyme A at the carboxylgroup before a nucleophilic substitution at the para position of the aromatic ring can take place.…”

Section: Chloroacrylic Acid Dehalogenationmentioning

confidence: 99%

Degradation of halogenated aliphatic compounds: The role of adaptation

Pries

Ploeg

Dolfing

et al. 1994

FEMS Microbiology Reviews

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A limited number of halogenated aliphatic compounds can serve as a growth substrate for aerobic microorganisms. Such cultures have (specifically) developed a variety of enzyme systems to degrade these compounds. Dehalogenations are of critical importance. Various heavily chlorinated compounds are not easily biodegraded, although there are no obvious biochemical or thermodynamic reasons why microorganisms should not be able to grow with any halogenated compound. The very diversity of catabolic enzymes present in cultures that degrade halogenated aliphatics and the occurrence of molecular mechanisms for genetic adaptation serve as good starting points for the evolution of catabolic pathways for compounds that are currently still resistant to biodegradation.

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Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Arthrobacter sp. strain SU

Abstract: Strains ofArthrobacter catalyze a hydrolytic dehalogenation of 4-chlorobenzoate (4-CBA) to p-hydroxybenzoate. The reaction requires ATP and coenzyme A (CoA), indicating activation of the substrate via a thioester, like that reported for Pseudomonas sp. strain CBS3 (J.

Cited by 76 publications

References 23 publications

Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium

Identification of genes coding for hydrolytic dehalogenation in the metagenome derived from a denitrifying 4-chlorobenzoate degrading consortium

Construction and Characterization of Two Recombinant Bacteria That Grow on ortho - and para -Substituted Chlorobiphenyls

Degradation of halogenated aliphatic compounds: The role of adaptation

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