Degradation of halogenated aliphatic compounds: The role of adaptation

Pries, Frens; Ploeg, van der Jan; Dolfing, Jan; Janssen, Dick B.

doi:10.1111/j.1574-6976.1994.tb00140.x

Cited by 26 publications

(8 citation statements)

References 109 publications

(100 reference statements)

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“…Another biotic factor that can significantly affect the in situ bioremediation process is 'adaptation of degradative microorganism(s) towards environmental stresses' (Fiorenza & Ward, 1997;Rittmann et al, 2001;Somova et al, 2005). The in situ application of microorganisms exposes them to diverse stresses that can lead to a major decline in the survival of the degradative microbial strain as well as the efficiency of the pollutant removal (Pries et al, 1994). One of the most common stresses encountered during in situ bioremediation is the elevated concentrations of target pollutant and cross-contamination of other nontargeted pollutants (Lee & Lin, 2006).…”

Section: Biotic Factorsmentioning

confidence: 99%

Integrative approaches for assessing the ecological sustainability ofin situbioremediation

2009

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Application of microbial metabolic potential (bioremediation) is accepted as an environmentally benign and economical measure for decontamination of polluted environments. Bioremediation methods are generally categorized into ex situ and in situ bioremediation. Although in situ bioremediation methods have been in use for two to three decades, they have not yet yielded the expected results. Their limited success has been attributed to reduced ecological sustainability under environmental conditions. An important determinant of sustainability of in situ bioremediation is pollutant bioavailability. Microbial chemotaxis is postulated to improve pollutant bioavailability significantly; consequently, application of chemotactic microorganisms can considerably enhance the performance of in situ degradation. The environmental fate of degradative microorganisms and the ecological consequence of intervention constitute other important descriptors for the efficiency and sustainability of bioremediation processes. Integrative use of culture-dependent, culture-independent methods (e.g. amplified rDNA restriction analysis, terminal restriction fragment length polymorphism, denaturing/thermal gradient gel electrophoresis, phospholipid fatty acid, etc.), computational and statistical analyses has enabled successful monitoring of the above aspects. The present review provides a detailed insight into some of the key factors that affect the efficiency of in situ bioremediation along with a comprehensive account of the integrative approaches used for assessing the ecological sustainability of processes. The review also discusses the possibility of developing suicidal genetically engineered microorganisms for optimized and controlled in situ bioremediation.

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Section: Biotic Factorsmentioning

confidence: 99%

Integrative approaches for assessing the ecological sustainability ofin situbioremediation

2009

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“…Inorganic materials, predominantly aluminas and silicas, have been commonly used as enzyme carriers because of their low cost, large surface area to volume ratio, favorable surface chemistry, and formability. In many studies involving similar haloalkane dehalogenases isolated from various bacteria (Janssen et al, 1985;Pries et al, 1994, Van den Wijngaard et al, 1992, each has demonstrated an activity optimum between pHs 8.2 and 9.5. Inorganic alumina supports perform well under these basic reaction conditions (Hyndman et al, 1992a(Hyndman et al, , 1992b.…”

Section: Introductionmentioning

confidence: 99%

Haloalkane hydrolysis with an immobilized haloalkane dehalogenase

Dravis

Swanson

Russell

2001

Biotech & Bioengineering

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Haloalkane dehalogenase from Rhodococcus rhodochrous was covalently immobilized onto a polyethyleneimine impregnated gamma-alumina support. The dehalogenating enzyme was found to retain greater than 40% of its original activity after immobilization, displaying an optimal loading (max. activity/supported protein) of 70 to 75 mg/g with an apparent maximum (max. protein/support) of 156 mg/g. The substrate, 1,2,3-trichloropropane, was found to favorably partition (adsorb) onto the inorganic alumina carrier (10 to 20 mg/g), thereby increasing the local reactant concentration with respect to the catalyst's environment, whereas the product, 2,3-dichloropropan-1-ol, demonstrated no affinity. Additionally, the inorganic alumina support exhibited no adverse effects because of solvent/component incompatibilities or deterioration due to pH variance (pH 7.0 to 10.5). As a result of the large surface area to volume ratio of the support matrix and the accessibility of the bound protein, the immobilized biocatalyst was not subject to internal mass transfer limitations. External diffusional restrictions could be eliminated with simple agitation (mixing speed: 50 rpm; flux: 4.22 cm/min). The pH-dependence of the immobilized dehalogenase was essentially the same as that for the native enzyme. Finally, both the thermostability and resistance toward inactivation by organic solvent were improved by more than an order of magnitude after immobilization.

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“…Rate measurements showed that haloalkane dehalogenase had an activity with various C1 and C2 haloalkanes (Table 2) that is slightly lower than the rate found with 1,2-dichloroethane, which was 4.9 lmol of substrate per min per mg of protein, close to the value of 6 lmol min )1 mg )1 found by Pries et al (1994). Especially compounds containing a carbon-bromine bond were rapidly converted.…”

Section: Dehalogenation Of Bcm Bce and Cfe By Haloalkane Dehalogenasementioning

confidence: 66%

Metabolism of mono- and dihalogenated C1 and C2 compounds by Xanthobacter autotrophicus growing on 1,2-dichloroethane

et al. 2006

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The conversion of and toxic effects exerted by several mono- and dihalogenated C1 and C2 compounds on cultures of Xanthobacter autotrophicus GJ10 growing on 1,2-dichloroethane were investigated. Bromochloromethane, dibromomethane and 1-bromo-2-chloroethane were utilized by strain GJ10 in batch culture as a cosubstrate and sole carbon source. The rate of degradation of dihalomethanes by whole cells was lower than that of 1,2-dichloroethane, but a significant increase of the rate of dihalomethane biodegradation was observed when methanol or ethanol were added as a cosubstrate. Products of the degradation of several tested compounds by haloalkane dehalogenase were analyzed and a new metabolic pathway based on hydrolytic conversion to formaldehyde was proposed for the dihalomethanes. Strain GJ10 growing on 1,2-dichloroethane converted 2-fluoroethanol and 1-chloro-2-fluoroethane to 2-fluoroacetate, which was tolerated up to a concentration of 2.5 mM. On the basis of the results from batch cultures an inert (dichloromethane), a growth-supporting (dibromomethane) and a toxic (1,2-dibromoethane) compound were selected for testing their effects on a continuous culture of strain GJ10 growing on 1,2-dichloroethane. The compounds were added as pulses to a steady-state chemostat and the response of the culture was followed. The effects varied from a temporary decrease in cell density for dibromomethane to severe toxicity and culture washout with 1,2-dibromoethane. Our results extend the spectrum of halogenated C1 and C2 compounds that are known to be degraded by strain GJ10 and provide information on toxic effects and transformation of compounds not serving as a carbon source for this bacterium.

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Degradation of halogenated aliphatic compounds: The role of adaptation

Cited by 26 publications

References 109 publications

Integrative approaches for assessing the ecological sustainability ofin situbioremediation

Integrative approaches for assessing the ecological sustainability ofin situbioremediation

Haloalkane hydrolysis with an immobilized haloalkane dehalogenase

Metabolism of mono- and dihalogenated C1 and C2 compounds by Xanthobacter autotrophicus growing on 1,2-dichloroethane

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