Cloning and sequence analysis of complementary DNA encoding a precursor for chicken natriuretic peptide

Akizuki, Naoya; Kangawa, Kenji; Minamino, Naoto; Matsou, Hisayuki

doi:10.1016/0014-5793(91)80331-v

Cited by 30 publications

(16 citation statements)

References 14 publications

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“…Since BNP is the only natriuretic peptide that has not been identified in eels, VNP could be defined as novel if a distinction between VNP and BNP could be clarified. It has been shown that all BNP cDNAs from mammals and birds so far reported invariably have seven repetitive ATTTA sequences in the 3' non-coding region (Kojima et al 1989;Akizuki et al 1991), which is known to accelerate mRNA degradation (Wilson & Treisman, 1988). Thus, the cDNA sequence of VNP could provide more definite information on the distinction between VNP and BNP.…”

Section: Introductionmentioning

confidence: 95%

Eel ventricular natriuretic peptide: cDNA cloning and mRNA expression

Takei

Ueki²,

Nishizawa³

1994

Journal of Molecular Endocrinology

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Ventricular natriuretic peptide (VNP), a newly discovered type of cardiac natriuretic peptide identified in eels, has a unique amino acid sequence and biological activity compared with other members of the natriuretic peptide family. We have cloned a cDNA encoding the eel VNP precursor from a cDNA library of eel ventricles and determined its sequence. Sequence analysis showed that the preproVNP consists of 150 amino acid residues containing a signal sequence of 22 amino acid residues at its N terminus and mature VNP(1-36) at its C terminus. Comparison with two types of mammalian cardiac natriuretic peptides (A and B type natriuretic peptides; ANP and BNP) revealed that VNP showed greater overall sequence identity to ANP than to BNP at both the cDNA and amino acid sequence levels. Since VNP cDNA lacks the repetitive ATTTA sequence in the 3' non-coding region, which is a characteristic common to all BNP cDNAs sequenced to date, VNP may differ from BNP. While mRNA for eel ANP was detected only in the atrium, that for eel VNP was more abundant in the ventricle than in the atrium. Thus VNP is regarded as the first truly ventricular hormone in terms of synthesis and storage. Southern blot analysis indicated that VNP is also present in the quail, which belongs to a taxon where BNP has already been identified.

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Section: Introductionmentioning

confidence: 95%

Eel ventricular natriuretic peptide: cDNA cloning and mRNA expression

Takei

Ueki²,

Nishizawa³

1994

Journal of Molecular Endocrinology

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“…However, the cDNA of eel VNP lacks the seven repetition of ATTTA sequence in the 3' non-coding region (Takei Y. and Nishizawa T., unpublished data). The presence of the repetitive sequence is a characteristic of all BNP cDNAs so far sequenced (Akizuki et al 1991). It remains to be determined whether the fourth natriuretic peptide, namely BNP, is present in the eel or not.…”

Section: Biochemistry Of Eel Anpmentioning

confidence: 99%

Biochemistry and physiology of a family of eel natriuretic peptides

Takei

Balment

1993

Fish Physiol Biochem

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Three natriuretic peptides with similar structures were isolated from eels and their amino acid sequences determined; atrial natriuretic peptide (ANP) from atria, ventricular natriuretic peptide (VNP) from ventricles, and C-type natriuretic peptide (CNP) from brains. All three hormones were circulating in eel blood, and their plasma levels invariably decreased when eels were transferred from fresh water (FW) to seawater (SW). Eel ANP and VNP inhibited drinking in FW and SW eels. Eel ANP inhibited water and Na(+) absorption by the intestine of SW eels. The potency of these ANP effects was 2-3 orders greater than those of other hormones which are known to have similar effects. Eel ANP and VNP induced antidiuresis but not antinatriuresis in FW eels. Eel ANP increased plasma cortisol level in SW eels but not in FW eels. The antidiuretic effect and the stimulation of cortisol secretion in eels are opposite to the ANP effects reported in mammals. These data suggest that ANP plays a complex role in the eel osmoregulation.

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“…We note that the timing of Fig. 1 Alignment of the atrial natriuretic factor predicted protein sequence from Xenopus, Rana (D01043), human (Nemer et al 1984) and chick (Akizuki et al 1991). The first 23 amino acids of Xenopus ANF (overlined) correspond to the signal sequence.…”

mentioning

confidence: 99%

Expression of atrial natriuretic factor ( ANF ) during Xenopus cardiac development

Small¹,

Krieg²

2000

Development Genes and Evolution

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We have isolated the Xenopus orthologue of the atrial natriuretic factor (ANF) gene. Characterization of embryonic expression indicates that the ANF gene is initially expressed throughout the developing myocardium at the late heart tube stage (about stage 32). This is in contrast to all previously characterized Xenopus cardiac differentiation markers that are first expressed in the cardiogenic plate at approximately stage 27. ANF expression becomes restricted exclusively to the atrium at about stage 47, long after the commencement of beating and the original formation of the atrial and ventricular compartments, but shortly after septation of the single atrium into two distinct atria.

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Cloning and sequence analysis of complementary DNA encoding a precursor for chicken natriuretic peptide

Cited by 30 publications

References 14 publications

Eel ventricular natriuretic peptide: cDNA cloning and mRNA expression

Eel ventricular natriuretic peptide: cDNA cloning and mRNA expression

Biochemistry and physiology of a family of eel natriuretic peptides

Expression of atrial natriuretic factor ( ANF ) during Xenopus cardiac development

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