Cloning and molecular characterization of apical efflux transporters (ABCB1, ABCB11 and ABCC2) in rainbow trout (Oncorhynchus mykiss) hepatocytes

Žaja, Roko; Munić, Vesna; Klobučar, Roberta Sauerborn; Ambriović-Ristov, Andreja; Smital, Tvrtko

doi:10.1016/j.aquatox.2008.09.012

Cited by 66 publications

(44 citation statements)

References 41 publications

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“…et al, 2006). However, functions of piscine ABCC2 proteins in the detoxification of toxicants remain to be defined, although several ABCC2 cDNAs have been cloned from fishes include rainbow trout (Oncorhynchus mykiss) (Zaja et al, 2008) and little skate (Leucoraja erinacea) (Cai et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and functions of zebrafish ABCC2 in cellular efflux of heavy metals

Long

Zhong

et al. 2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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Section: Discussionmentioning

confidence: 99%

Molecular characterization and functions of zebrafish ABCC2 in cellular efflux of heavy metals

Long

Zhong

et al. 2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…MRP5 is involved in the detoxification of platinum (Weaver et al, 2005) and cadmium (McAleer et al, 1999). In aquatic organisms, a number of MRP transporters have been identified from invertebrates (Sauerborn et al, 2004) and fishes (Fischer et al, 2010;Zaja et al, 2008). These transporters appear to play an important role in multi-xenobiotic resistance (Loncar et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

Long

Wang

2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…In aquatic organisms, the presence of ABC transporters which are involved in MXR has been demonstrated [16]. In addition to extensively studied MDR1, several ABC transporters such as ABCC2 [17,18], ABCC3 [19] and BCRP [20] have been reported in fish. However, to our knowledge, functions of ABCC1 in fish remain to be characterized.…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis and heavy metal detoxification of ABCC1/MRP1 in zebrafish

Long

Cui

2010

Mol Biol Rep

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ABCC1/MRP1 belongs to the ATP-binding cassette superfamily and its elevated expression is closely associated with the multidrug resistance of various tumor cells. In normal tissues, ABCC1 confers resistance to a wide variety of xenobiotics and toxicants, demonstrating its important roles in tissue defense. Here, we report the cloning and functional characterization of abcc1 gene in zebrafish. This gene is localized on zebrafish chromosome 3 and contains a 4,557 bp open-reading frame. The deduced polypeptide is composed of 1,518 amino acids, which shares 70% identity with human ABCC1. Phylogenetic analysis revealed that ABCC1 proteins from thirteen vertebrate species are highly conserved during evolution. Transcriptional expression of zebrafish abcc1 gene in developing embryos was examined by whole-mount in situ hybridization and real-time PCR. Transcripts of zebrafish abcc1 gene were detectable in four-cell stage embryos, indicating that this gene is maternally expressed. ABCC1 mRNAs were ubiquitously distributed in embryos before 12 h post-fertilization (hpf) and mainly localized in eyes and brain from 24 to 72 hpf, and in gills from 96 to 120 hpf. In addition, zebrafish abcc1 gene was highly expressed in 1-hpf embryos and detected in all adult tissues examined, with highest expression in testis and lowest in heart and liver. Exposure of ZF4 cells and embryos to CdCl 2 Á2.5H 2 O, HgCl 2 , Pb(NO 3 ) 2 , or Na 3 AsO 4 Á12H 2 O significantly induced transcriptional expression of abcc1 gene. Furthermore, overexpression of abcc1 improved the survival rates of embryos exposed to Cd, Hg or As, while overexpression of a abcc1 mutant (ABCC1-G1420D) sensitized zebrafish embryos to toxic metals. These data indicate that zebrafish ABCC1 has crucial roles in heavy metals detoxification.

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Cloning and molecular characterization of apical efflux transporters (ABCB1, ABCB11 and ABCC2) in rainbow trout (Oncorhynchus mykiss) hepatocytes

Cited by 66 publications

References 41 publications

Molecular characterization and functions of zebrafish ABCC2 in cellular efflux of heavy metals

Molecular characterization and functions of zebrafish ABCC2 in cellular efflux of heavy metals

MRP proteins as potential mediators of heavy metal resistance in zebrafish cells

Molecular analysis and heavy metal detoxification of ABCC1/MRP1 in zebrafish

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