Molecular characterization and functions of zebrafish ABCC2 in cellular efflux of heavy metals

Long, Yong; Li, Qing; Zhong, Shan; Wang, Youhui; Cui, Zongbin

doi:10.1016/j.cbpc.2011.01.002

Cited by 55 publications

(71 citation statements)

References 63 publications

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“…The identification of these transporters and their putative role in the defense against natural and man-made substances has been investigated in more than 40 aquatic species (e.g., rainbow trout) (Lončar et al, 2010). In addition, a number of these ABC genes have been identified and studied in several fish species including zebrafish (Long et al, 2011), Nile tilapia (Costa et al, 2012), killifish (Christine Paetzold et al, 2009), and Antarctic fish (Zucchi et al, 2010). Previous studies have determined the tissue expression patterns of ABCC2 in zebrafish (Long et al, 2011) and little skate (Cai et al, 2003), and ABCB1 in the sheepshead minnow (Hemmer et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, a number of these ABC genes have been identified and studied in several fish species including zebrafish (Long et al, 2011), Nile tilapia (Costa et al, 2012), killifish (Christine Paetzold et al, 2009), and Antarctic fish (Zucchi et al, 2010). Previous studies have determined the tissue expression patterns of ABCC2 in zebrafish (Long et al, 2011) and little skate (Cai et al, 2003), and ABCB1 in the sheepshead minnow (Hemmer et al, 1998). Only a few studies have assessed the expression profiles of other toxicologically relevant ABC transporters in fish, e.g., ABCB11 and ABCG2 (Lončar et al, 2010;Costa et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…A few studies have evaluated the transcriptional effects on ABC gene expression in several fish species exposed to PAHs (Christine Paetzold et al, 2009;Costa et al, 2012), PFOS (Diaz de Cerio et al, 2012), and heavy metals (Zucchi et al, 2010;Long et al, 2011). Up-regulation of the ABCC2 and ABCG2 transcripts in the Nile tilapia (Costa et al, 2012) and killifish (Christine Paetzold et al, 2009) exposed to PAHs has been reported.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, an increase in the ABCB1 mRNA level was observed in Antarctic fish exposed to Cd (Zucchi et al, 2010). Nevertheless, the regulation and function of these ABC transporters in fish are still relatively unclear (Lončar et al, 2010;Long et al, 2011;Costa et al, 2012). The induction of the phase I (e.g., CYP1A) and phase II (e.g., GSTs, GCLG, and UGTs) xenobiotic-metabolizing enzyme genes by environmental pollutants (e.g., PAHs) has been widely studied (Van der Oost et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Despite the physiological importance of the toxicologically relevant ABC transporters and their role in cellular defense, their expression patterns, mechanisms of regulation and biological functions have not been fully characterized in fish (Lončar et al, 2010;Long et al, 2011;Costa et al, 2012). Therefore, the aims of the present study were to identify five toxicologically relevant ABC transporter genes in rare minnow, characterize their basal expression patterns across eight different tissues (liver, gill, intestine, kidney, spleen, brain, skin, and muscle), and assess the transcriptional effects of pyrene exposure on these ABC genes as well as several xenobioticmetabolizing enzyme genes in the liver of the Chinese rare minnow.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Transcriptional expression analysis of ABC efflux transporters and xenobiotic-metabolizing enzymes in the Chinese rare minnow

Yuan

Zha

et al. 2014

Environmental Toxicology and Pharmacology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptional expression analysis of ABC efflux transporters and xenobiotic-metabolizing enzymes in the Chinese rare minnow

Yuan

Zha

et al. 2014

Environmental Toxicology and Pharmacology

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Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity

et al. 2020

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ATP‐binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy‐based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium‐throughput fashion.

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An engineered abcb4 expression model reveals the central role of NF‐κB in the regulation of drug resistance in zebrafish

Sun

et al. 2022

Drug Development Research

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Multi-drug resistance (MDR) is a phenomenon that tumor cells are exposed to a chemotherapeutic drug for a long time and then develop resistance to a variety of other anticancer drugs with different structures and different mechanisms. The in vitro studies of tumor cell lines cannot systematically reflect the role of MDR gene in vivo, and the cost of in vivo studies of transgenic mice as animal models is high. Given the myriad merits of zebrafish relative to other animal models, we aimed to establish a screening system using zebrafish stably expressing ATP-binding cassette (ATP-cassette) superfamily transporters and unveil the potential regulatory mechanism. We first used the Tol2-mediated approach to construct a Tg (abcb4:EGFP) transgenic zebrafish line with ATP-binding cassette (ABC) subfamily B member 4 (abcb4) gene promoter to drive EGFP expression. The expression levels of abcb4 and EGFP were significantly increased when Tg(abcb4:EGFP) transgenic zebrafish embryos were exposed to doxorubicin (DOX) or vincristine (VCR), and the increases were accompanied by a marked decreased accumulation of rhodamine B (RhB) in embryos, indicating a remarkable increase in DOX or VCR efflux. Mechanistically, Akt and Erk signalings were activated upon the treatment with DOX or VCR. With the application of Akt and Erk inhibitors, drug resistance was reversed with differing responsive effects. Notably, downstream NF-κB played a central role in the regulation of abcb4-mediated drug resistance. Taken together, the data indicate that the engineered Tg(abcb4:EGFP) transgenic zebrafish model is a new platform for screening drug resistance in vivo, which may facilitate and accelerate the process of drug development.

show abstract

Molecular characterization and functions of zebrafish ABCC2 in cellular efflux of heavy metals

Cited by 55 publications

References 63 publications

Transcriptional expression analysis of ABC efflux transporters and xenobiotic-metabolizing enzymes in the Chinese rare minnow

Transcriptional expression analysis of ABC efflux transporters and xenobiotic-metabolizing enzymes in the Chinese rare minnow

Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity

An engineered abcb4 expression model reveals the central role of NF‐κB in the regulation of drug resistance in zebrafish

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