Cloning and molecular characterization of calpain, a calcium-activated neutral proteinase, from different strains of Schistosoma japonicum

Zhang, Renli; Suzuki, Takashi; Takahashi, Satoru; Yoshida, Ayako; Kawaguchi, Hitoshi; Maruyama, Haruhiko; Yabu, Yoshisada; Fu, Jun; Shirai, Tomoyuki; Ohta, Nobuhiro

doi:10.1016/s1383-5769(99)00024-0

Cited by 16 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that calpain could have enough immunogenicity for both humoral and cellular responses. A previous experiment performed in our laboratory indicated that human sera from S. japonicum-infected individuals recognized r-calpain, and sera obtained from mice immunized with r-calpain showed enhanced binding to cercarial antigens (38). Together with these findings, the principal objective of our present study was to develop a livestock vaccine that can be used to prevent Asian schistosomiasis.…”

mentioning

confidence: 53%

“…A recombinant molecule of the large subunit of calpain from S. japonicum was prepared as described previously (38). In brief, cDNA encoding amino acid residues 219 to 376 of S. japonicum calpain was amplified by reverse transcription (RT)-PCR because a comparable portion was shown to be highly immunogenic in murine schistosomiasis mansoni (17).…”

Section: Methodsmentioning

confidence: 99%

“…Calpain from S. mansoni was shown to induce protective immunity during murine experimental schistosomiasis mansoni (11), and molecular cloning of calpain from S. japonicum has since started in several laboratories, including our own (28,38). Although calpain is believed to be an intracellular protease, the location of this molecule seems not to be fixed and in some cases it is moved outside of the cell membrane (26).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

et al. 2001

Self Cite

View full text Add to dashboard Cite

A large subunit of calpain, a calcium-activated neutral proteinase, from Schistosoma japonicum was cloned and expressed in Escherichia coli. When BALB/c mice were immunized with purified recombinant calpain (r-calpain) emulsified in complete Freund's adjuvant, a significant reduction in the number of recovered worms and also in egg production per female worm was observed (P < 0.01). Spleen cells of the immunized mice showed enhanced production of gamma interferon (IFN-␥) by activated CD4 ؉ T cells. Considering our observation of elevated expression of inducible nitric oxide synthase mRNA in immunized mice, r-calpaininduced IFN-␥ seemed to upregulate the production of nitric oxide by macrophages and subsequently mediated the killing of schistosomulae in the lung. On the other hand, spleen cells of immunized mice showed only faint interleukin-4 production in response to r-calpain in vitro, suggesting that immunization with r-calpain alters the Th1-Th2 balance in murine hosts even during a Th2-promoting S. japonicum infection. Furthermore, histopathological study of the livers of immunized mice showed that granulomas formed around eggs were diminished in both size and number. Egg production by female worms was clearly decreased in immunized mice, suggesting that r-calpain also has antifecundity effects. Taken together, these results point to S. japonicum calpain as a potential vaccine candidate for both worm killing and disease prevention, possibly through the induction of a strong Th1-dominant environment in immunized mice.More than 200 million people have schistosomiasis, and almost 600 million people are exposed to the risk of schistosomiasis (36). Chemotherapy is currently the choice for control; however, vaccine development remains an important long-term goal for the integrated control of schistosomiasis because of high reinfection rates in areas where the disease is endemic. Extensive work has been carried out to identify schistosome molecules that confer partial but significant protection in different animal models. These include the Schistosoma mansoni 28-kDa and the S. japonicum 26-kDa glutathione S-transferase (GST) (5, 32), the S. mansoni and S. japonicum 97-kDa paramyosin (13, 25), the S. mansoni 28-kDa triose phosphate isomerase (29), the S. mansoni 23-kDa integral membrane antigen (24), and so forth. These vaccine candidates were selected by the World

show abstract

mentioning

confidence: 53%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

et al. 2001

Self Cite

View full text Add to dashboard Cite

show abstract

“…S. japonicum (Yamanashi strain) was maintained by passage through Oncomelania nosophora and BALB/C mice (15,16). The pairs of adult worms were recovered from the portal vein of the infected mice and cultured as 1 pair/well of 12-well culture plates in RPMI 1640 medium supplemented with 5 or 10% human serum in 5% CO 2 atmosphere, as described previously (10).…”

Section: Parasites and Egg Embryonation In Vitromentioning

confidence: 99%

CD36‐related protein inSchistosoma japonicum: candidate mediator of selective cholesteryl ester uptake from high‐density lipoprotein for egg maturation

Okumura-Noji

Miura

et al. 2012

FASEB j.

Self Cite

View full text Add to dashboard Cite

Familial cholesteryl ester transfer protein (CETP) deficiency is more common in some East Asian populations than elsewhere, suggesting the possibility of a selective advantage of this genetic defect against regional infectious diseases. Historically, infection with the Asian blood fluke Schistosoma japonicum has been endemic in these regions, including Japan. We previously reported that eggs of S. japonicum require cholesteryl ester uptake from normal high-density lipoprotein (HDL) but not from CETP-deficient HDL for their maturation to miracidia, a critical step of the hepatic pathogenesis of schistosomiasis. Herein we show that cholesteryl ester uptake is selective from HDL, and identified CD36-related protein (CD36RP) as a candidate to mediate the reaction. CD36RP was cloned from the adult and the egg developmental stages of S. japonicum, with 1880 bp encoding 506 amino acid residues exhibiting the CD36 domains and two transmembrane regions. Using antibodies against recombinant peptides representing the potential extracellular domains of CD36RP, Western blotting detected a protein with a molecular mass of 82 kDa in the particulate fraction of the adult parasite cells, which was reduced to 62 kDa after N-glycanase treatment. The extracellular domain peptide bound human HDL, as established by immunoblots following nondenaturing gel electrophoresis. Antibodies against the extracellular domain suppressed HDL cholesteryl ester uptake and maturation of the eggs in vitro. CD36RP is a candidate receptor on eggs of S. japonicum that facilitates uptake of HDL cholesteryl ester necessary for egg embryonation and maturation.

show abstract

“…Human antibody responses to the large subunit of schistosomal calpain have also been associated with resistance. In humans infected with S. japonicum -presenting light infections, a strong reactivity to calpain was observed whereas in individuals with stronger infection, low reactivity to calpain was observed ( 81 ). In a study in an endemic area for schistosomiasis in Egypt, IFN-γ production in response to Sm14 and IgE and IgA antibodies against Sm28GST correlated with resistance to infection ( 82 ).…”

Section: What Can We Learn From the Studies In Humans?mentioning

confidence: 99%

Eliminating Schistosomes through Vaccination: What are the Best Immune Weapons?

2015

View full text Add to dashboard Cite

The successful development of vaccines depends on the knowledge of the immunological mechanisms associated with the elimination of the pathogen. In the case of schistosomes, its complex life cycle and the mechanisms developed to evade host immune system, turns the development of a vaccine against the disease into a very difficult task. Identifying the immunological effector mechanisms involved in parasite attrition and the major targets for its response is a key step to formulate an effective vaccine. Recent studies have described some promising antigens to compose a subunit vaccine and have pointed to some immune factors that play a role in parasite elimination. Here, we review the immune components and effector mechanisms associated with the protective immunity induced by those vaccine candidates and the lessons we have learned from the studies of the acquired resistance to infection in humans. We will also discuss the immune factors that correlate with protection and therefore could help to evaluate those vaccine formulations in clinical trials.

show abstract

Cloning and molecular characterization of calpain, a calcium-activated neutral proteinase, from different strains of Schistosoma japonicum

Cited by 16 publications

References 25 publications

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

CD36‐related protein inSchistosoma japonicum: candidate mediator of selective cholesteryl ester uptake from high‐density lipoprotein for egg maturation

Eliminating Schistosomes through Vaccination: What are the Best Immune Weapons?

Contact Info

Product

Resources

About