Cloning and functional study of lipocalin: retinol-binding protein-like gene family of the ridgetail white prawn, Exopalaemon carinicauda

Ma, Hangke; Sun, Jinqiu; Xu, Wanyuan; Gao, Wei; Hu, Guangwei; Lai, Xiaofang; Yan, Binlun; Gao, Huan

doi:10.1007/s00438-019-01633-0

Cited by 14 publications

(5 citation statements)

References 37 publications

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“…By searching our previously established transcriptomic database for E. carinicauda (Ma, Sun, et al, 2020), we obtained the suspected CDK 9 gene core sequence. The full‐length cDNA sequence of CDK 9 gene was then obtained by 3′ and 5′ Race techniques according to the SMARTer RACE 5′/3’ Kit (TaKaRa, Japan).…”

Section: Methodsmentioning

confidence: 99%

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Zhang¹,

Li²,

Zheng³

et al. 2022

Aquaculture Research

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To investigate the key genes affecting ovarian development in the ridgetail white prawn, Exopalaemon carinicauda, we cloned the full‐length cDNA of the CDK9 gene and discussed its role in ovarian development. Our results show that the gene has an open reading frame of 1155 bp, encoded 383 amino acids, and it has a conserved cyclin binding motif S_TKc. The open reading frame of CDK9 was expressed as a 43.96 Kda molecular weight protein. The phylogenetic tree analysis showed that the CDK9 gene has the highest homology with the CDK9 genes of Penaeus vannamei and Penaeus japonicus. The results of qRT‐PCR show that the CDK9 gene was also expressed in the eye stalk, stomach, heart, gill, intestine, muscle, ovary, hepatopancreas and ventral cord. The expression level was highest in the ovary, which was significantly different from the other tissues (p < 0.05). In the five stages of ovarian development, the expression level of the CDK9 gene was highest in stage I, which was significantly different from the other stages (p < 0.05). In situ hybridization tests showed strong expression signals in the membrane, cytoplasm and nucleus of oocytes and previtellogenic oocytes. Nevertheless, following the proliferation of oocytes, the expression signals became much stronger in the oocyte membrane and in the whole follicular cells located in the interstitial space. The RNA interference results show that the expression of the CDK9 gene significantly affects the expression of the Vg and VgR genes. Our findings suggest that CDK9 plays an important role in the synthesis and transportation of vitellin.

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Section: Methodsmentioning

confidence: 99%

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Zhang¹,

Li²,

Zheng³

et al. 2022

Aquaculture Research

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“…All target mRNA levels were normalized to the 18S RNA levels and expressed as a relative level. Relative gene expression levels were evaluated using the

2^{‐ normalΔ normalΔ C_{T}}

method (Ma et al, 2020; Raja et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…The ridgetail white prawn ( Exopalaemon carinicauda ), widely spread offshore of South Korea and China, is one of the most important commercial shrimp species in China (Deng et al, 2021; Ge et al, 2021; Liang et al, 1988). It has an expanding scale of culturing, because of its fast growth and strong environmental adaptability (Ma et al, 2020; Wang et al, 2020). According to statistical data from 2016, its total aquaculture area in China is about 40,000 hm 2 and its annual production is about 100,000 tons (Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary short‐chain fatty acid salts on the growth performance, digestive, antioxidant and immune enzyme activities, immune‐related gene expression and resistance to Vibro parahaemolytics infection in juvenile ridgetail white prawn ( Exopalaemon carinicauda )

Deng¹,

Duan²,

Mao³

et al. 2021

Aquac Res

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An 8‐week feeding trial was conducted to study the effects of dietary short‐chain fatty acid salts, sodium acetate (SA), sodium propionate (SP) and sodium butyrate (SB), on the growth performance, digestive ability, immune parameters and disease resistance of juvenile Exopalaemon carinicauda. Shrimps (14.60 ± 1.48 mg) were fed with 10 experimental diets containing 0.0 (control), 0.25%, 0.5% and 1% of SA, SB and SP in triplicate. The highest survival rate was observed in the SP (0.5%) group (p < 0.05). The final weight, specific growth rate and feed efficiency was highest in the SP (0.25%) group (p < 0.05). Shrimps that were fed SP (0.25%) had greater intestinal digestive enzyme (chymotrypsin) activity. Shrimps in the SA (0.5%) group had the highest activities of glutathione peroxidase, catalase, lysozyme, acid phosphatase and alkaline phosphatase, which was accompanied by the highest relative percent survival after a Vibro parahaemolyticus challenge (p < 0.05). The expression of immune‐related genes (prophenoloxidase, C‐type lectin and mannose binding lectin) in the hepatopancreas of shrimps fed SA (0.5%) and SP (0.25%) was higher than that of the control group (p < 0.05). These data suggest that SA (0.5%) and SP (0.25%) are beneficial to the growth performance and health of E. carinicauda.

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“…Exopalaemon carinicauda, belonging to Arthropoda, Crustacea, Decapoda, Nematoda, and Palaemonidae, is an important economic shrimp in China [14,15]. It is a potentially ideal model organism for the study of marine shrimp, as its growth physiology, reproductive development, and genetic breeding have been widely studied [16][17][18][19][20][21][22]. At present, the infection of EHP is also found in the process of cultivation of E. carinicauda, but there are still few drug studies on the treatment of EHP.…”

Section: Introductionmentioning

confidence: 99%

Effect of Exogenous Nitric Oxide on Enterocytozoon hepatopenaei Copy Numbers and Immunity of Exopalaemon carinicauda

Jiang¹,

Li²,

Xu³

et al. 2023

Aquaculture Research

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To explore the effect of nitric oxide (NO) on Enterocytozoon hepatopenaei (EHP) invading the shrimp, sodium nitroprusside was used as an exogenous NO donor to investigate the effects of different concentrations (0 μg/L (control group), 0.3 μg/L, 0.6 μg/L, and 0.9 μg/L) of sodium nitroprusside on the EHP copy number and the structure in the hepatopancreas of Exopalaemon carinicauda infected by EHP. The results showed that the EHP copy number in the sodium nitroprusside group was significantly lower than that in the control group, and the hepatopancreas in the sodium nitroprusside injection group had less structure damage than the control group. Additionally, superoxide dismutase (SOD) activity in the control group and 0.3 μg/L groups increased and then decreased, while alkaline phosphatase (AKP) activity decreased and then increased, with the same trend, and, the inducible nitric oxide synthase (iNOS) activity in the haemolymph of the 0.3 μg/L group was significantly higher than that of the control group at 3 days ( P < 0.05 ). The NO content the 0.3 μg/L group was also higher from 1 to 4 days than that in the control group and significantly higher than that in the control group at 3 days ( P < 0.05 ). The injection of NO could reduce the EHP copy number in infected E. carinicauda and slow down the pathological changes in the hepatopancreas structure, which might be related to the increase in NO content.

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Cloning and functional study of lipocalin: retinol-binding protein-like gene family of the ridgetail white prawn, Exopalaemon carinicauda

Cited by 14 publications

References 37 publications

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

Effects of dietary short‐chain fatty acid salts on the growth performance, digestive, antioxidant and immune enzyme activities, immune‐related gene expression and resistance to Vibro parahaemolytics infection in juvenile ridgetail white prawn ( Exopalaemon carinicauda )

Effect of Exogenous Nitric Oxide on Enterocytozoon hepatopenaei Copy Numbers and Immunity of Exopalaemon carinicauda

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