Effects of dietary short‐chain fatty acid salts on the growth performance, digestive, antioxidant and immune enzyme activities, immune‐related gene expression and resistance to
            <i>Vibro parahaemolytics</i>
            infection in juvenile ridgetail white prawn (
            <i>Exopalaemon carinicauda</i>
            )

Deng, Gaowei; Duan, Jiancheng; Mao, Hua; Yan, Binlun; Chen, Hao; Hu, Guangwei; Gao, Wei; Gao, Huan

doi:10.1111/are.15542

Cited by 14 publications

(8 citation statements)

References 38 publications

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“…The amplification reactions for quantitative real-time polymerase chain reaction (qRT-PCR) were performed in 96-well plates using a total volume of 20 μ L, containing 0.4 μ L of each primer, 4 μ L of cDNA template, 10 μ L of 2 × ChamQ SYBR qPCR Master Mix (Vazyme Biotech Co., Ltd., China), 0.4 μ L of ROX Reference Dye (Vazyme Biotech Co., Ltd., China), and 4.8 μ L of sterilized double-distilled water. The qRT-PCR was performed with an ABI StepOnePlus Real-Time PCR System using the following cycle conditions: 95°C for 30 s, followed by 40 cycles of 95°C for10 s, and 60°C for 30 s [ 34 ]. Melting curve analysis was carried out to validate that only one PCR product was obtained in these reactions.…”

Section: Methodsmentioning

confidence: 99%

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Mao¹,

Yang²,

Chen³

et al. 2023

Aquaculture Nutrition

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An 8-week feeding trial was performed to evaluate the effects of dietary β-hydroxy-β-methylbutyrate (HMB) supplementation on growth performance and muscle quality of kuruma shrimp (Marsupenaeus japonicas) (initial weight: 2.00 ± 0.01 g ) fed a low protein diet. The positive control diet (HP) with 490 g/kg protein and negative control diet (LP) with 440 g/kg protein were formulated. Based on the LP, 0.25, 0.5, 1, 2 and 4 g/kg β-hydroxy-β-methylbutyrate calcium were supplemented to design the other five diets named as HMB0.25, HMB0.5, HMB1, HMB2 and HMB4, respectively. Results showed that compared with the shrimp fed LP, the HP, HMB1 and HMB2 groups had significantly higher weight gain and specific growth rate, while significantly lower feed conversion ratio ( p < 0.05 ). Meanwhile, intestinal trypsin activity was significantly elevated in the above three groups than that of the LP group. Higher dietary protein level and HMB inclusion upregulated the expressions of target of rapamycin, ribosomal protein S6 kinase, phosphatidylinositol 3-kinase, and serine/threonine-protein kinase in shrimp muscle, accompanied by the increases in most muscle free amino acids contents. Supplementation of 2 g/kg HMB in a low protein diet improved muscle hardness and water holding capacity of shrimp. Total collagen content in shrimp muscle increased with increasing dietary HMB inclusion. Additionally, dietary inclusion of 2 g/kg HMB significantly elevated myofiber density and sarcomere length, while reduced myofiber diameter. In conclusion, supplementation of 1-2 g/kg HMB in a low protein diet improved the growth performance and muscle quality of kuruma shrimp, which may be ascribed to the increased trypsin activity and activated TOR pathway, as well as elevated muscle collagen content and changed myofiber morphology caused by dietary HMB.

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Section: Methodsmentioning

confidence: 99%

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Mao¹,

Yang²,

Chen³

et al. 2023

Aquaculture Nutrition

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“…SCFAs used as feed additives increased phagocytosis index, lysozyme activity, and immunity-related genes (IL1-β, TNF-α, and TGF-β) in fish (Hoseinifar et al, 2016 b). Moreover, it has been shown that SCFA-binding G protein-coupled receptors are mainly expressed in innate immune cells and have been suggested as a possible mechanism for the effects of SCFAs on mammalian immunity (Deng et al, 2021;.…”

Section: Effects Of Dietary Xoss On Immune System Parametersmentioning

confidence: 99%

Prebiotic effects of dietary xylooligosaccharides on fish gut microbiota, growth, and immunological parameters – A review

Gufe¹,

Merrifield

Hoseinifar

et al. 2024

Annals of Animal Science

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Xylooligosaccharides (XOSs) are increasingly being explored as prebiotics in fish diets; however, their effects and modes of action have not been well evaluated. Reports have shown that dietary XOS has the potential to improve the proliferation of beneficial gut microbes, and their metabolites, and enhance disease resistance in several fish species. In contrast, other studies report no substantial changes in immune and growth parameters compared to control groups. Like all prebiotics, the mode of action of XOS is based on their selective stimulation of beneficial gut microbiota, which will outcompete and prevent pathogen proliferation in the gut, and produce metabolites that modulate host immune responses. The reports of improved growth performance of XOS fed fish may be due improved intestinal microbiome, enhanced glycolysis activity and elevated gastrointestinal enzymatic activities. Dietary XOSs have different effects on fish performance depending on the fish species and the structure of XOSs (degree of XOS polymerization and substitution). Nevertheless, further research is essential to determine the optimal dosage, degree of polymerization, and substitution levels required to improve each fish species' gut health and growth performance. This review highlights the prebiotic effects of XOSs, their mechanism of action, and knowledge gaps.

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“…It has proven to be a very important breed for fishery farmers, because of its good environmental adaptability, rapid growth, and high economic value (Liang et al, 2012;Gao et al, 2021). At present, the annual output of E. carinicauda is about 50 000 tonnes (Deng et al, 2021). However, with the expansion of the breeding scale of E. carinicauda, the outbreaks of diseases caused by bacteria or viruses are becoming more frequent, which has caused serious financial losses to farmers.…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression analysis of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae) in embryonic development and immune response to bacteria

Huang¹,

Xu²,

Zhu³

et al. 2023

Crustac.

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β-catenin is an important signal transduction protein in the canonical Wnt/β-catenin pathway, which is widely involved in the development of animals. However, the role of β-catenin in crustaceans is still unclear. In this study, the cDNA of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) was identified and characterized. The open reading frame (ORF) is 2424 bp, which encodes 807 amino acids containing an N-terminal region of a GSK-β consensus phosphorylation site and a central region of 11 armadillo (ARM) repeats. The tissue distribution showed that β-catenin was highly expressed in ovary, testis, hepatopancreas, heart, and gills. During embryonic development, the transcript of β-catenin was relatively high in the zygote, and the thirty-two-cell stage, blastula stage, gastrula stage, and nauplius stage. The transcripts of β-catenin in haemocytes, gills, and hepatopancreas were significantly up-regulated after infection with Vibrio anguillarum Bergeman, 1909. These results indicate that β-catenin participates in the regulation of embryonic development and in the immune response in E. carinicauda.

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Effects of dietary short‐chain fatty acid salts on the growth performance, digestive, antioxidant and immune enzyme activities, immune‐related gene expression and resistance to Vibro parahaemolytics infection in juvenile ridgetail white prawn ( Exopalaemon carinicauda )

Cited by 14 publications

References 38 publications

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Prebiotic effects of dietary xylooligosaccharides on fish gut microbiota, growth, and immunological parameters – A review

Characterization and expression analysis of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae) in embryonic development and immune response to bacteria

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