Cloning and functional characterization of the ovine malic enzyme promoter

Stefos, Georgios C; Becker, Walter; Lampidonis, Antonis D.; Rogdakis, E.

doi:10.1016/j.gene.2008.09.027

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Since the cell culture metabolic studies were performed in high glucose media we expect ME1, a lipogenic enzyme (Stefos et al, 2009), to be the predominant pathway for recycling TCA intermediates to pyruvate and allowing for complete oxidation of fatty acids. According to this hypothesis, the flux through ME1 is necessary for the increased fatty acid degradation in ACE cells.…”

Section: Resultsmentioning

confidence: 99%

Resistance to Diet-Induced Obesity in Mice with Synthetic Glyoxylate Shunt

et al. 2009

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Summary Given the success in engineering synthetic phenotypes in microbes and mammalian cells, constructing non-native pathways in mammals has become increasingly attractive for understanding and identifying potential targets for treating metabolic disorders. Here we introduced the glyoxylate shunt into mouse liver to investigate mammalian fatty acid metabolism. Mice expressing the shunt showed resistance to diet-induced obesity on a high fat diet despite similar food consumption. This was accompanied by a decrease in total fat mass, circulating leptin levels, plasma triglyceride concentration, and a signaling metabolite in liver, malonyl-CoA, that inhibits fatty acid degradation. Contrary to plants and bacteria, in which the glyoxylate shunt prevents the complete oxidation of fatty acids, this pathway when introduced in mice increases fatty acid oxidation such that resistance to diet-induced obesity develops. This work suggests that using non-native pathways in higher organisms to explore and modulate metabolism may be a useful approach.

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Section: Resultsmentioning

confidence: 99%

Resistance to Diet-Induced Obesity in Mice with Synthetic Glyoxylate Shunt

et al. 2009

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“…When the human ME1 T3RE was introduced into the ovine ME1 promoter context, transcriptional activity was increased in the hepatic cell lines HepG2 and H4IIE but not in differentiated 3T3-L1 cells. These results suggest that the sequence of the T3RE in the ME1 promoter determines differences in the tissue/species activity of malic enzyme in ruminants and human [107]. This functional difference may be related to the differential contribution of liver and adipose tissue in whole body lipogenesis between humans and sheep as a ruminant species.…”

Section: Focusing On Molecular Levelmentioning

confidence: 99%

Comparative Approach of the de novo Fatty Acid Synthesis (Lipogenesis) between Ruminant and Non Ruminant Mammalian Species: From Biochemical Level to the Main Regulatory Lipogenic Genes

Laliotis

Bizelis²,

Rogdakis³

2010

113

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Over the second half of 20th century much research on lipogenesis has been conducted, especially focused on increasing the production efficiency and improving the quality of animal derived products. However, many diferences are observed in the physiology of lipogenesis between species. Recently, many studies have also elucidated the involvement of numerous genes in this procedure, highlighting diferences not only at physiology but also at the molecular level. The main scope of this review is to point out the major differences between ruminant and non ruminant species, that are observed in key regulatory genes involved in lipogenesis. Human is used as a central reference and according to the findinggs, main differences are analysed. These findings could serve not only as basis for understanding the main physiology of lipogenesis and further basic research, but also as a basis for any animal scientist to develop new concepts and methods for use in improving animal production and modern genetic improvement.

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“…In ruminants, contrary to humans and rodents, the pathway of glucose-pyruvate-acetylcoA is of little significance, as the principal carbon source for lipogenesis instead of glucose, is the acetic acid produced by the rumen's microorganisms (Bergen and Mersmann, 2005). In sheep, unlike to other species, two transcripts encoding ovine Me-1 has been reported (Stefos et al, 2009), which may further elucidate possible explanations for the minor role of cytosolic malic enzyme in these species. It is therefore likely to expect the little expression of this enzyme in the studied breeds.…”

Section: Discussionmentioning

confidence: 98%

Comparative Physiological and Molecular Study of Some Sheep Breeds in Saudi Arabia

AL-Harbi

Alwagdany

Amer

2013

American Journal of Biochemistry and Biotechnology

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Naemi, Heri, Najdi and Sawakni sheep breeds inhabiting Kingdom of Saudi Arabia were studied on both physiological and molecular bases. Native polyacrylamide gel-electrophoreses for two enzymes Malate Dehydrogenase (Mdh) and Malic Enzyme (Me) recorded 5 isoenzyme loci from which 4 were polymorphic and 1 was monomorphic. Mdh showed three fractions, the first was recorded in Sawakni and Najdi breeds, the second was monomorphic and was recorded in all breeds while the third was recorded in all except Heri. Malic enzyme showed two polymorphic fractions, the first was recorded in Heri and Naemi and the second was recorded in all breeds. Both metabolic enzymes activities were higher in Sawakni than in the native breeds. 607, 227, 498 and 595 bp nucleotides from CO1, ND4, cytb genes and d-loop, respectively, were sequenced with no base substitutions among and/or within breeds except for ND4 gene and d-loop. The molecular tree clustered the three breeds other than Naemi in one group and Naemi was basal. Isoenzymes therefore, discriminate clearly the studied breeds although they are not as accurate as molecular tools indicating their applicability with more efficiency in physiology than in genetics while the d-loop was efficient in discriminating the studied breeds phynotypically

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Cloning and functional characterization of the ovine malic enzyme promoter

Cited by 3 publications

References 27 publications

Resistance to Diet-Induced Obesity in Mice with Synthetic Glyoxylate Shunt

Resistance to Diet-Induced Obesity in Mice with Synthetic Glyoxylate Shunt

Comparative Approach of the de novo Fatty Acid Synthesis (Lipogenesis) between Ruminant and Non Ruminant Mammalian Species: From Biochemical Level to the Main Regulatory Lipogenic Genes

Comparative Physiological and Molecular Study of Some Sheep Breeds in Saudi Arabia

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