Cloning and expression of an antifungal chitinase gene of a novel Bacillus subtilis isolate from Taiwan potato field

Yang, Chi-Yea; Ho, Yi Cheng; Pang, Jen-Chieh; Huang, Shiang-Suo; Tschen, Johannes Seng-Ming

doi:10.1016/j.biortech.2008.07.039

Cited by 37 publications

(17 citation statements)

References 21 publications

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“…pumilus (Dehestani et al 2010) and chi18 (97%) from Bac. subtilis CHU26 (Yang et al 2009). Both studies showed the inhibitory effects of these chitinases on the growth of plant pathogenic fungi and expression of plant diseases.…”

Section: Discussionmentioning

confidence: 97%

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Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113

et al. 2011

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Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) increased in vitro inhibition of the plant pathogenic fungi Rhizoctonia solani, Fusarium oxysporum f.sp. vasinfectum, Rhizoctonia cerealis, Bipolaris sorokiniana, Verticillium dahliae and Gaeumannomyces graminis var. tritici compared with P418. In planta disease suppression assays indicated that P418-37 significantly (P < 0.05) enhanced suppression of wheat sheath blight (R. cerealis), cotton Fusarium wilt (F. oxysporium f.sp. vasinfectum) and tomato gray mould (Botrytis cinerea), relative to the wild type.

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Section: Discussionmentioning

confidence: 97%

“…amyloliquefaciens (Wang et al 2002) and Bac. subtilis CHU26 (Yang et al 2009) inhibited Fusarium oxysporum and Rhizoctonia solani, respectively.…”

Section: Introductionmentioning

confidence: 98%

Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113

et al. 2011

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“…Chitinases produced by the Gram-negative soil bacterium Serratia marcescens have been studied extensively (Zakariassen et al 2009 ) . On the other hand, understanding of the chitinases from Gram-positive bacteria of the genus Bacillus is scarce although secretion of chitinases have been reported from several species such as B. circulans (Hashimoto et al 2000 ) , B. subtilis (Yang et al 2009 ) , B. alvei , B. pumilus (Ahmadian et al 2007 ) , B. lentus , B. licheniformis (Chuang et al 2008 ) , B. cereus , B. amyloliquifaciens (Wang et al 2002 ) and B. thuringiensis (BarbozaCorona et al 2008 ) and other Bacillus sp. (Lee et al 2008 ) .…”

Section: Microbial Chitinases Hydrolytically Cleave Chitinmentioning

confidence: 98%

Microbial Chitinases for Chitin Waste Management

Das

Neeraja

Sarma

et al. 2011

Microorganisms in Environmental Management

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Chitin is a major structural component of fungi and exoskeleton of insects, crustaceans and other arthropods. It is an insoluble, unbranched, linear chain of b -1, 4-linked N-acetyl D-glucosamine residues and is the second most abundant renewable carbohydrate polymer in nature after cellulose and fi rst in marine environment. The annual production of chitin in aquatic biosphere is around 10 11 ton. Chitinases produced by chitinolytic bacteria have the potential to convert this waste to pharmaceutically valuable end products such as N-acetyl glucosamine and chitooligosaccharides, and are viable alternatives of chemical processes currently used for the purpose. Chitinases from different bacteria, fungi, plants and animals are glycosyl hydrolases which degrade the insoluble chitin in to soluble chitooligosaccharides and glucosamine. Chitooligosaccharides possess antitumor, antifungal, antibacterial and immuno-enhancing effects . Antagonistic bacteria and chitinases have been exploited as potential biocontrol agents against fungal pathogens in plants. The growing number of application areas for chitin and chitin-derived products demand an equally diverse array of chitin-modifying enzymes for specifi c needs. The chapter will focus on the applications of chitinolytic enzymes from microbial sources and their possible applications, with special focus on conversion of large quantities of the chitinous substrates into useful biological products.

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“…In particular, bacteria produce various chitinases to digest chitin and utilize it as an energy source (3), and most of these chitinases have been isolated and characterized from Bacillus species (1,4,5). Bacillus species secrete a large number of chitinases into the medium, which complicate downstream processing and affect product stability (1,4).…”

Section: Introductionmentioning

confidence: 99%

Overexpression and characterization of thermostable chitinase from Bacillus atrophaeus SC081 in Escherichia coli

Cho

Choi²,

Choi

2011

BMB Reports

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The chitinase-producing strain SC081 was isolated from Korean traditional soy sauce and identified as Bacillus atrophaeus based on a phylogenetic analysis of the 16S rDNA sequence and a phenotypic analysis. A gene encoding chitinase from B. atrophaeus SC081 was cloned in Escherichia coli and was named SCChi-1 (GQ360078). The SCChi-1 nucleotide sequences were composed of 1788 base pairs and 596 amino acids, which were 92.6, 89.6, 89.3, and 78.9% identical to those of Bacillus subtilis (ABG57262), Bacillus pumilus (ABI15082), Bacillus amyloliquefaciens (ABO15008), and Bacillus licheniformis (ACF40833), respectively. A recombinant SCChi-1 containing a hexahistidine tag at the amino-terminus was constructed, overexpressed, and purified in E. coli to characterize SCChi-1. H6SCChi-1 revealed a hydrolytic band on zymograms containing 0.1% glycol chitin and showed the highest lytic activity on colloidal chitin and acidic chitosan. The optimal temperature and pH for chitinolytic activity were 50 o C and pH 8.0, respectively. [BMB reports 2011; 44(3) : 193-198]

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Cloning and expression of an antifungal chitinase gene of a novel Bacillus subtilis isolate from Taiwan potato field

Cited by 37 publications

References 21 publications

Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113

Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113

Microbial Chitinases for Chitin Waste Management

Overexpression and characterization of thermostable chitinase from Bacillus atrophaeus SC081 in Escherichia coli

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