Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113

Abstract

Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) increased in vitro inhibition of the plant pathogenic fungi Rhizoctonia solani, Fusarium oxysporum f.sp. vasinfectum, Rhizoctonia cerealis, Bipolaris sorokiniana, Verticillium dahliae and Gaeumannomyces graminis var. tritici compared with P418. In planta disease suppression assays indicated that P418-37 significantly (P < 0.05) enhanced suppression of wheat sheath blight (R. cerealis), cotton Fusarium wilt (F. oxysporium f.sp. vasinfectum) and tomato gray mould (Botrytis cinerea), relative to the wild type.

Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its Application to Improve Plant Growth under Nitrogen-Deficient Conditions

“…For instance, genetic engineering has been applied to increase the production of auxin and antifungal compounds in Pseudomonas, Azospirillum and Bacillus strains [24], [25], [26], [27], [28], [29], [30], [31]. Regarding genetic engineering for nitrogen fixation, some authors began to investigate the transfer of nif genes among different strains belonging to the phylum Proteobacteria .…”

El género Bacillus como agente de control biológico y sus implicaciones en la bioseguridad agrícola

“…Also, genetic engineering studies focused on the increase of antifungal activity of Bacillus strains have successfully achieved the insertion of codifying genes into lytic enzymes, such as gene ChiA of B. subtilis F29-3 in B. circulans, obtaining a more aggressive phenotype against Botrytis elliptica (Chen et al, 2004). In addition, Zhang et al (2012) significantly increased the antifungal activity of Burkholderia vietnamiensis P418 in the face of different fungal phytopathogens by the chromosomal insertion of the gene Chi113, from a B. Subtilis strain.…”

“…Además, estudios de ingeniería genética enfocados a incrementar la actividad antifúngica de cepas de Bacillus han logrado exitosamente la inserción de genes codificantes a enzimas líticas, tal como el gen ChiA de B. subtilis F29-3 en B. circulans, obteniendo un fenotipo más agresivo contra Botrytis elliptica (Chen et al, 2004). Además, Zhang et al (2012) incrementaron significativamente la actividad antifúngica de Burkholderia vietnamiensis P418 frente a diferentes fitopatógenos fúngicos mediante la inserción cromosómica de gen Chi113, proveniente de una cepa de B. subtilis.…”

Overexpression of Chitinase A Gene from Serratia marcescens in Bacillus subtilis and Characterization of Enhanced Chitinolytic Activity

“…Rostami and coauthors [12] expressed chitinase gene from B. pumilus on the spore surface of B. subtilis, and showed its growth inhibitory activity on R. solani and Trichoderma harzianum. Chitinase gene from B. subtilis was also expressed in E. coli [13,16] or Burkholderia vietnamiensis [17].…”