Cloning and expression of Allophycocyanin gene from Gracilariopsis lemaneiformis and studying the binding sites of phycocyanobilin on its α and β subunits

Guo, Yalin; Zang, Xiaonan; Cao, Xuexue; Feng, Zhang; Sun, Deguang; Shang, Menghui; Li, Rui; Yangzong, Zhaxi; Wei, Xuehong; Zhang, Xuecheng

doi:10.1007/s10811-020-02102-7

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Research into APC seems to be yet quite scarce when compared with research targeted on the other PBP found in red seaweeds, and it seems to be more focused on understanding mechanisms of structure [107,141,142] and synthesis [33,143]. Nevertheless, regarding extraction methods, diethyl ether gave good results, but methanol and ethanol were deemed improper in APC extraction of Furcelaria lumbricalis [74].…”

Section: Allophycocyaninmentioning

confidence: 99%

Red Seaweed Pigments from a Biotechnological Perspective

et al. 2021

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Algae taxa are notably diverse regarding pigment diversity and composition, red seaweeds (Rhodophyta) being a valuable source of phycobiliproteins (phycoerythrins, phycocyanin, and allophycocyanin), carotenes (carotenoids and xanthophylls), and chlorophyll a. These pigments have a considerable biotechnological potential, which has been translated into several registered patents and commercial applications. However, challenges remain regarding the optimization and subsequent scale-up of extraction and purification methodologies, especially when considering the quality and quantity needs, from an industrial and commercial point of view. This review aims to provide the state-of-the-art information on each of the aforementioned groups of pigments that can be found within Rhodophyta. An outline of the chemical biodiversity within pigment groups, current extraction and purification methodologies and challenges, and an overview of commercially available products and registered patents, will be provided. Thus, the current biotechnological applications of red seaweeds pigments will be highlighted, from a sustainable and economical perspective, as well as their integration in the Blue Economy.

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Section: Allophycocyaninmentioning

confidence: 99%

Red Seaweed Pigments from a Biotechnological Perspective

et al. 2021

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“…At present, there have been many reports on the expression of phycocyanin and allophycocyanin from different species of cyanobacteria, but they mainly focus on exploring the activity and function of individual phycocyanin or allophycocyanin ( 34 , 35 ). The study of co-recombination expression has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence and antioxidant activity of heterologous expression of phycocyanin and allophycocyanin from Arthrospira platensis

Shang

Sun

et al. 2023

Front. Nutr.

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Phycocyanin and allophycocyanin are important active substances in Arthrospira platensis, because of their fluorescent characteristic and antioxidant capacity. In order to solve the problem of insufficient production and inconvenient modification of natural protein, recombinant expression was performed and the fluorescence activity and antioxidant activity was analyzed to meet the demand for phycocyanin and allophycocyanin. A total of seven recombinant strains were constructed in this study, including individual phycocyanin or allophycocyanin, co-expression of phycocyanin-allophycocyanin, and their co-expression with chromophore, and the expression strain for individual chromophore. Different molecular weights of phycocyanin and allophycocyanin were detected in the recombinant strains, which indicated the different polymers expressed. Through mass spectrometry identification, phycocyanin and allophycocyanin may form a dimer of 66 kDa and a polymer of 300 kDa. The results of fluorescence detection showed that phycocyanin and allophycocyanin combined with phycocyanobilin to show fluorescence activity. The fluorescence peak of recombinant phycocyanin was mainly concentrated at 640 nm, which was similar to natural phycocyanin, the fluorescence peak of purified recombinant allophycocyanin was at about 642 nm. The fluorescence peak of the co-expressed recombinant phycocyanin-allophycocyanin is located at 640 nm, and the fluorescence intensity is between the recombinant phycocyanin and the recombinant allophycocyanin. After purification, the fluorescence peak of the recombinant phycocyanin is more concentrated and the fluorescence intensity is higher, which is about 1.3 times of recombinant phycocyanin-allophycocyanin, 2.8 times of recombinant allophycocyanin, indicating that phycocyanin may be more suitable to be used as fluorescence probe in medicine. The antioxidant capacity was measured by using total antioxidant capacity (T-AOC) and DPPH (2,2'-diphenyl-1-triphenylhydrazino) free radical scavenging method, and the recombinant phycobiliprotein showed antioxidant activity. Phycocyanobilin also has certain antioxidant activity and could enhance the antioxidant activity of phycobiliprotein to a certain extent. Recombinant phycocyanin-allophycocyanin polymer has stronger T-AOC, which is about 1.17–2.25 times that of the other five recombinant proteins. And recombinant phycocyanin has stronger DPPH antioxidant activity, which is about 1.2–2.5 times that of the other five recombinant proteins. This study laid the foundation for the application of recombinant phycocyanin and allophycocyanin in medical detection and drug development.

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Cloning of apcE gene from Arthrospira platensis FACHB314 and its function in heterologous expression

Shang

et al. 2022

J Appl Phycol

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Cloning and expression of Allophycocyanin gene from Gracilariopsis lemaneiformis and studying the binding sites of phycocyanobilin on its α and β subunits

Cited by 5 publications

References 39 publications

Red Seaweed Pigments from a Biotechnological Perspective

Red Seaweed Pigments from a Biotechnological Perspective

Fluorescence and antioxidant activity of heterologous expression of phycocyanin and allophycocyanin from Arthrospira platensis

Cloning of apcE gene from Arthrospira platensis FACHB314 and its function in heterologous expression

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