Cloning and Expression of a Novel Murine Anti-human Fas Antibody

Yoshida-Kato, Hiroko; Ichikawa, Kimihisa; Yamaguchi, Junko; Watanabe, Kenji; Ohsumi, Jun; Yonehara, Shin; Serizawa, Nobufusa

doi:10.1271/bbb.64.1903

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…HFE7A, mouse anti-human/mouse Fas mAb was obtained as mentioned previously Yoshida-kato et al 2000). BALB/c mice were purchased from Charles River Japan (Yokohama, Japan).…”

Section: Animals and Antibodiesmentioning

confidence: 99%

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Protective effects of HFE7A, mouse anti-human/mouse Fas monoclonal antibody against acute and lethal hepatic injury induced by Jo2

et al. 2009

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“…HFE7A, mouse anti-human/mouse Fas mAb was obtained as mentioned previously Yoshida-kato et al 2000). BALB/c mice were purchased from Charles River Japan (Yokohama, Japan).…”

Section: Animals and Antibodiesmentioning

confidence: 99%

“…We have obtained the unique mouse anti-Fas mAb HFE7A, which reacts with both human and mouse Fas Yoshida-Kato et al 2000). Although administration of HFE7A induced apoptosis in thymocytes, it did not show any signs of hepatotoxicity in mice .…”

Section: Introductionmentioning

confidence: 99%

Protective effects of HFE7A, mouse anti-human/mouse Fas monoclonal antibody against acute and lethal hepatic injury induced by Jo2

et al. 2009

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“…A murine monoclonal antibody (mAb) HFE7A (mHFE7A), raised against human Fas, binds to both human and mouse Fas (11,12). Notably, mHFE7A induced apoptosis in thymocytes in mice without any overt signs of hepatotoxicity, which is known to occur with other anti-Fas agents including the hamster anti-murine Fas mAb, JO2 (10).…”

Section: Introductionmentioning

confidence: 99%

mHFE7A, a newly identified monoclonal antibody to Fas, induces apoptosis in human melanoma cells in vitro and delays the growth of melanoma xenotransplants

Hiramoto

Inui²,

Kamei³

et al. 2006

Oncol Rep

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Abstract. The Fas receptor is a potentially valuable therapeutic target in cancer treatment. However, the clinical application of antibodies directed to this target is hindered by their serious side effects in vivo, including liver toxicity. One murine monoclonal antibody, mHFE7A, binds both to human Fas and murine Fas, without inducing any obvious side effects. However, the potential therapeutic effects of mHFE7A are unclear in human cancer cells or tumors. Here, we determined whether mHFE7A could induce apoptosis in vitro, and assessed its effects on major apoptotic pathways in a human melanoma cell line, MMN9. We also investigated its anti-cancer effects on transplanted melanoma MMN9 tumors in BALB/c nude mice. Treatment of mHFE7A cross-linking with Ig induced cell death similar to CH-11 treatment. Apoptosis induced by mHFE7A was defined by Hoechst 33342 DNA staining and DNA fragmentation assay. Furthermore, mHFE7A-mediated apoptosis was dependent on activation of a caspase signaling cascade involving caspases-8 and -3. Administration of mHFE7A also delayed the growth of melanoma xenografts. Thus, we provide the first evidence that the murine anti-Fas monoclonal antibody, mHFE7A, induces apoptosis of human malignant melanoma cells in vitro and is anti-tumorigenic in vivo.

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“…The mouse antibody HFE7A (m-HFE7A), raised against human Fas, binds to both human and mouse Fas (Ichikawa et al, 2000;Yoshida-Kato et al, 2000). Administration of m-HFE7A induces apoptosis in thymocytes in mice.…”

Section: Introductionmentioning

confidence: 99%

Crystallization and preliminary X-ray crystallographic studies on a Fab fragment of the mouse anti-human Fas monoclonal antibody HFE7A

Ito

Takayama

Hanzawa

et al. 2001

Acta Crystallogr D Biol Cryst

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The Fas-Fas ligand system is involved in apoptosis. The mouse anti-human Fas monoclonal antibody HFE7A (m-HFE7A) has a potential use in human therapy against autoimmune diseases such as rheumatoid arthritis. Information on the three-dimensional structure is essential for antibody humanization. Crystals of an antigen-binding fragment (Fab) of m-HFE7A were obtained by the hanging-drop vapour-diffusion method using sodium citrate as a precipitant and 2-methyl-2,4-pentanediol as an additive. Fast optimization to produce single crystals suitable for X-ray analysis was achieved by the streak-seeding technique. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 43.4, b = 74.0, c = 133.8 A. The crystals diffract at least to 2.5 A resolution.

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Cloning and Expression of a Novel Murine Anti-human Fas Antibody

Cited by 4 publications

References 15 publications

Protective effects of HFE7A, mouse anti-human/mouse Fas monoclonal antibody against acute and lethal hepatic injury induced by Jo2

Protective effects of HFE7A, mouse anti-human/mouse Fas monoclonal antibody against acute and lethal hepatic injury induced by Jo2

mHFE7A, a newly identified monoclonal antibody to Fas, induces apoptosis in human melanoma cells in vitro and delays the growth of melanoma xenotransplants

Crystallization and preliminary X-ray crystallographic studies on a Fab fragment of the mouse anti-human Fas monoclonal antibody HFE7A

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