Cloning and expression analysis of Mx cDNA from Senegalese sole (Solea senegalensis)

Photobacterium damselae subsp. damselae, an Emerging Pathogen Affecting New Cultured Marine Fish Species in Southern Spain

“…In last 7 years, several new marine fish species are being evaluated as potential candidates for aquaculture production. In Southern Spain, studies on the reproductive cycles, nutrition, growth, histology and immune system of species such as Senegelese sole (Solea senegalensis), redbanded seabream (Pagrus auriga), common seabream (Pagrus pagrus), white seabream (Diplodus sargus), and meagre (Argyrosomus regius) are ongoing (Cardenas & Calvo, 2003;Prieto et al, 2003;Ponce et al, 2004;Manchado et al, 2005;Fernandez-Trujillo et al, 2006;Martin-Antonio et al, 2007;Cardenas & Manchado, 2008). However, the intensive culture of these new fish species has favoured the appearance of several outbreaks with varied mortality rates.…”

Mx expression in gilthead sea bream (Sparus aurata L.) in response to poly I:C, bacterial LPS and chromosomal DNA: Preliminary study

“…Mx genes have been cloned and characterised from several other mammals, birds and fish species [4], such as rainbow trout, Atlantic salmon, Atlantic halibut, Japanese flounder, pufferfish, sea bream [5] and Senegalese sole [6].…”

In vitro inhibition of sole aquabirnavirus by Senegalese sole Mx

“…The sole Mx cDNA (SsMx) [21] was cloned between ApaI and NotI sites of the expression vector pcDNA4/HisMax (Invitrogen). The green fluorescent protein (GFP) gene was introduced in the same vector, between EcoRI and NotI restriction sites.…”

“…Our group has recently cloned the Mx cDNA from Senegalese sole, Solea senegalensis (Kaup, 1858) [21], a species of growing importance in European aquaculture [22,23]. Furthermore, as a consequence of its intensive culture, an increasing number of viruses are being recorded from this fish species, such as an aquabirnavirus related to infectious pancreatic necrosis virus (IPNV) Sp [24] named sole aquabirnavirus.…”